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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > DNA Ligation > DNA Ligation Kit £¼Mighty Mix£¾
°íÈ¿À²ÀÇ Ligation premix

DNA Ligation Kit £¼Mighty Mix£¾

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
6023
DNA Ligation Kit £¼Mighty Mix£¾
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 Kit
192,800¿ø 
241,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6023_e.v1811Da.pdf

Á¦Ç°¼³¸í
DNA Ligation Kit <Mighty Mix>´Â T4 DNA Ligase¿¡ ÀÇÇÑ ligation ¹ÝÀÀ¿¡ ÇÊ¿äÇÑ ½Ã¾àÀ» ¸ðµÎ Æ÷ÇÔÇÏ´Â 1 solution typeÀÇ premix ½Ã¾àÀÌ´Ù.
Ligation È¿À²À» ÃÖÀûÈ­ÇÑ ¹ÝÀÀ¾× Á¶¼ºÀ¸·Î °£ÆíÇÏ°Ô ´Ù¾çÇÑ ligation ¹ÝÀÀ¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.

  • Æí¸®ÇÑ 1 solution premix ŸÀÔ
    : Ligation Mix¿Í DNA ¿ë¾×°ú È¥ÇÕÇÏ´Â °ÍÀ¸·Î ´Ù¾çÇÑ À¯ÇüÀÇ ligation ¹ÝÀÀ °¡´É
  • Sticky end´Â »Ó¸¸ ¾Æ´Ï¶ó, Blunt end ligation, TA cloning¿¡µµ ÃÖÀû
  • 16¡É, 30ºÐÀÇ Ç¥ÁØ ÇÁ·ÎÅäÄÝ »Ó¸¸ ¾Æ´Ï¶ó, °£´ÜÇÑ plasmid vector ligation½Ã 25¡É, 5ºÐÀÇ °í¼Ó ÇÁ·ÎÅäÄÝ °¡´É
  • 50ȸÀÇ µ¿°áÀ¶ÇØ¿¡µµ ¼º´É ÀúÇÏ°¡ ¾øÀ½À» È®ÀÎ
³»¿ë (100ȸ, 1¹ÝÀÀ ´ç 7.5 §¡ »ç¿ë½Ã)

1.

Ligation Mix

150 §¡¡¿5

º¸Á¸ -20¡É
¹ÝÀÀ·® ¹× ¹ÝÀÀÁ¶°Ç

 

DNA¿ë¾×£ºLigation MixÀÇ ¿ë·®ºñ

¹ÝÀÀ Á¶°Ç

(1) Plasmid vector¿¡ ¿Ü·¡ DNA »ðÀÔ
¡¼Ç¥ÁØ ÇÁ·ÎÅäÄÝ¡½
¡¼°í¼Ó ÇÁ·ÎÅäÄÝ¡½

 

1£º1

16¡É, 30ºÐ
25¡É, 5ºÐ

(2) T vector¿¡ PCR»ê¹° cloning

1£º1

16¡É, 30ºÐ

(3) Linker Ligation, Adaptor Ligation
Plasmid vector¿¡ Linker DNA »ðÀÔ
cDNA¿¡ Adaptor, Linker Ligation

1£º1
1£º2

16¡É, 30ºÐ

(4) ¥ë phage vector¿¡ ¿Ü·¡ DNA »ðÀÔ*
(in vitro packing)

1£º2

26¡É, 10ºÐ


*DNA Ligation Kit Ver.1 (Code 6021)¿Í µ¿ÀÏÇÑ È¿À²À» ¾òÀ» ¼ö ÀÖÁö¸¸, »ç¿ë¾ç¿¡¼­´Â Ver.1 »ç¿ëÀÌ ´õ À¯¸®ÇÏ´Ù
¾ÈÁ¤¼º
º» Á¦Ç°ÀÇ µ¿°á À¶ÇØ¿¡ ÀÇÇÑ È¿À² º¯È­¸¦ È®ÀÎÇϱâ À§ÇØ pUC118- Hind III/BAP vector¿Í 564 bp Hind III ´ÜÆíÀÇ ligation ½ÇÇèÀ» ÁøÇàÇÑ °á°ú, 50 ȸÀÇ µ¿°áÀ¶ÇØ ¹Ýº¹¿¡µµ È¿À²ÀÇ ÀúÇÏ´Â ³ªÅ¸³ªÁö ¾Ê¾Ò´Ù.

°ü·ÃÁ¦Ç°
- High Fidelity PCR È¿¼Ò
      PrimeSTAR¢ç GXL DNA Polymerase (Code R050A/B)
      PrimeSTAR¢ç GXL Premix (Code R051A/B)
- °íÈ¿À² Competent Cells
      E. coli DH5¥á Competent Cells (Code 9057)
      E. coli HST08 Premium Competent Cells (Code 9128)
- Colony PCR¿ë dye plus premix
      EmeraldAmp¢ç GT PCR Master Mix (Code RR310A/B)

*Cloning ½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit - ¼±Åð¡ÀÌµå ¹Ù·Î°¡±â (Ŭ¸¯)

Keyword : T4 DNA ligase,ligase,ligase mix,cloining,¶óÀÌ°ÔÀÌÁî,Ŭ·Î´×

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