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Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type

GXL Premix ŸÀÔ

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R051A
PrimeSTAR¢ç GXL Premix
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(50 ul ±âÁØ)
270,400¿ø 
338,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R051A_DS.v1902Da.pdf
Takara
R051B
PrimeSTAR¢ç GXL Premix
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (R051A x 4)
1,216,000¿ø 


  • PrimeSTAR¢ç GXL DNA PolymeraseÀÇ ¼º´ÉÀ» ±×´ë·Î 2X premix ŸÀÔÀ¸·Î!
  • Primer¿Í ÁÖÇü DNA¸¸ ÷°¡ÇÏ¿© PCR ¹ÝÀÀÀ» ÁøÇàÇϹǷΠ»ç¿ëÀÌ Æí¸®
Á¦Ç°¼³¸í
PrimeSTAR¢ç GXL Premix´Â High-fidelity DNA polymeraseÀÎ PrimeSTAR¢ç GXL DNA Polymerase (Code R050A/B)¿Í reaction buffer, dNTP mixture°¡ ÃÖÀûÀÇ Á¶°ÇÀ¸·Î È¥ÇյǾî ÀÖ´Â 2X premixÀÌ´Ù.
º» Á¦Ç°Àº PrimeSTAR¢ç GXL DNA Polymerase°¡ °¡Áø Á¤È®¼º, long target ÇÕ¼º´É·Â (30 kb ÀÌ»ó), GC-rich region¿¡ ´ëÇÑ ÁõÆø¼º´É ¹× °ú·®ÀÇ template°¡ ÀÖ´Â »óÅ¿¡¼­µµ high fidelity·Î ÁõÆøÇÒ ¼ö ÀÖ´Â ¼º´ÉÀº À¯ÁöÇϸç, »ç¿ë Æí¸®¼ºÀ» ³ôÀÌ°í ´Ù¼ö »ùÇÃÀÇ ¹ÝÀÀ µî¿¡ ÀûÇÕÇϵµ·Ï premixÈ­ µÇ¾ú´Ù.
¶ÇÇÑ, º» È¿¼Ò´Â »ó¿Â¿¡¼­ DNA Polymerase È°¼º ¹× 3¡¯ ¡æ 5¡¯ exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â ´ÜÀÏ Å¬·Ð Ç×ü¸¦ ÀÌ¿ëÇÑ hot start versionÀÇ PCR È¿¼ÒÀÌ´Ù.

º» Á¦Ç°Àº 2¹è ºÐ·®ÀÇ PrimeSTAR¢ç GXL DNA Polymerase¸¦ »ç¿ëÇÏ´Â °í¼Ó PCR ÇÁ·ÎÅäÄÝ¿¡´Â ÀûÇÕÇÏÁö ¾ÊÀ¸¹Ç·Î, °í¼Ó PCRÀÌ ÇÊ¿äÇÑ °æ¿ì PrimeSTAR¢ç GXL DNA Polymerase (Code R050A/B)¸¦ ÃßõÇÕ´Ï´Ù.
³»¿ë (200 ȸ, 50 §¡ ¹ÝÀÀ½Ã)
1. PrimeSTAR GXL Premix (2X)

1 §¢ x 5

º¸Á¸
-20 ¡É (4 ¡É¿¡¼­ 3°³¿ù º¸°ü °¡´É)
°úµµÇÑ µ¿°áÀ¶ÇØÀÇ ¹Ýº¹À¸·Î È¿¼Ò È°¼ºÀÌ ³·¾ÆÁö´Â °æ¿ì°¡ ÀÖÀ¸´Ï, »ç¿ëºóµµ°¡ ³ôÀº °æ¿ì ÀÏ´Ü À¶ÇØÇÑ ½Ã¾àÀ» 4 ¡É º¸°üÇÏ´Â °ÍÀ» ÃßõÇÕ´Ï´Ù. »ç¿ë Àü Àß ¼¯¾î spin down ÈÄ »ç¿ëÇϼ¼¿ä.
PCR »ê¹°¿¡ ´ëÇÏ¿©
PrimeSTAR¢ç GXL Premix¸¦ ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº ÆòÈ° ¸»´Ü (Blunt End)ÀÌ´Ù.
µû¶ó¼­ T-vector¿¡ Ŭ·Î´× ÇÏ´Â °æ¿ì, PCR »ê¹°ÀÇ 3' ¸»´Ü¿¡ dA¸¦ ºÎ°¡ÇØ¾ß Çϸç, Mighty TA-cloning Reagent Set for PrimeSTAR¢ç(Code 6019)¸¦ ÀÌ¿ëÇÏ¿© A-tailing ¹× TA cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°À» ±×´ë·Î (ÇÊ¿ä¿¡ µû¶ó¼­ ÀλêÈ­ÇÏ¿©) ÆòÈ° ¸»´Ü º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ´Â °æ¿ì Mighty Cloning Reagent Set (Blunt End) (Code 6027)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- Á¤È®µµ ³ôÀº PCR È¿¼Ò: PrimeSTAR¢ç GXL DNA Polymerase
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit: PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword : PCRÈ¿¼Ò,Á¤È®µµ,high fidelity,high fidelity È¿¼Ò,Ŭ·Î´×,cloning,ligase,PCR mix,PCR premix,¹éÀü¹é½ÂPCR,¹éÀü¹é½Â PCR

High fidelity PCR: PrimeSTAR
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