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PCR
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RT-PCR °ü·Ã Á¦Ç°
> TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0
TaKaRa Ex Taq HS°ú AMV RTase¸¦ ÀÌ¿ëÇÑ 2 step RT-PCR ¿ë Kit
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0
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Takara
RR019A
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0
100 ȸ
431,200¿ø
539,000¿ø
09.02 ~ 10.31
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Takara
RR019B
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0
200 ȸ (RR019A x 2)
1,024,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
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AMV (Avian Myeloblastosis Virus) À¯·¡ÀÇ ¿ªÀü»ç È¿¼Ò AMV RTase XL¿¡ ÀÇÇÑ RNA·ÎºÎÅÍ cDNA ÇÕ¼º, ±×¸®°í TaKaRa Ex Taq HS¿¡ ÀÇÇÑ cDNA ÁõÆøÀ» ÇÑ°³ÀÇ Æ©ºê ³»¿¡¼ ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº RNA·ÎºÎÅÍÀÇ cDNA ÇÕ¼º°ú cDNA ÁõÆø¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀ¸·Î ±¸¼ºÇÑ kitÀÌ´Ù. AMV RTase XL´Â ¹ÝÀÀ ¿Âµµ¸¦ ³ô°Ô ¼³Á¤ÇÒ ¼ö Àֱ⠶§¹®¿¡ (42~ 60¡É) ±ä strandÀÇ cDNA ÇÕ¼º »Ó¸¸ ¾Æ´Ï¶ó º¹ÀâÇÑ 2Â÷ ±¸Á¶¸¦ °¡Áö±â ½¬¿î RNAÀÇ ¿ªÀü»ç ¹ÝÀÀ¿¡µµ À¯È¿ÇÏ´Ù. PCR ÁõÆø¿¡ »ç¿ëµÇ´Â TaKaRa Ex Taq HS´Â Hot Start PCR¿ë È¿¼ÒÀ̱⠶§¹®¿¡ ¹ÝÀÀ¾× Á¶Á¦½ÃÀÇ misannealingÀ̳ª primer dimer¿¡¼ À¯·¡ÇÏ´Â ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ¾î ³ôÀº ƯÀ̼ºÀÇ PCR ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Oligo dT-Adaptor Primer´Â poly(A)
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RNAÀÇ 3£§¸»´ÜºÎÅÍ cDNA¸¦ ³ôÀº È¿À²·Î ÇÕ¼ºÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾î ÀÖ¾î 3'-RACE¹ýÀ¸·Î ¹ÌÁöÀÇ 3' ¸»´ÜÀÇ ÁõÆøÀ» È¿°úÀûÀ¸·Î ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
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*1
AMV Reverse Transcriptase XL(5 U/ul)
50 ul
RNase Inhibitor(40 U/ul)
25 ul
Random 9 mers(50 pmol/ul)
50 ul
Oligo dT-Adaptor Primer(2.5 pmol/ul)
50 ul
RNase Free dH
2
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1 ml
TaKaRa Ex Taq HS(5 U/ul)
25 ul
M13 Primer M4(20 uM)
50 ul
10 ¡¿ RT Buffer
*2
1 ml
5 ¡¿ PCR Buffer
1 ml
dNTP Mixture (°¢ 10 mM)
150 ul
MgCl
2
(25 mM)
1 ml
Control F-1 Primer(20 pmol/ul)
*3
25 ul
Control R-1 Primer(20 pmol/ul)
*4
25 ul
Positive Control RNA(2¡¿10
5
copies/ul)
25 ul
*1
[10 ul ¿ªÀü»ç¹ÝÀÀ ¡æ 50 ul PCR ¹ÝÀÀ]ÀÇ 100ȸºÐ
*2
100 mM Tris-HCl(pH8.3), 500 mM KCl
*3
Positive Control RNA¿ë upstream primer
*4
Positive Control RNA ¿ë downstream primer
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RNA Template
Full length
ÁõÆø »çÀÌÁî
ÃÖ´ë 5 kbp Á¤µµ ÁõÆø°¡´É (
5.0 kb)
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AMV Reverse Transcriptase XL(42 ~ 60¡É¿¡¼ ¿ªÀü»ç°¡´É£©
DNA Polymerase
TaKaRa Ex Taq HS
RNase Inhibitor
ÇÊ¿ä(Kit³» Æ÷ÇÔ)
1st strand cDNA ÇÕ¼º primer
Random 9 mers, Oligo dT-Adaptor Primer, ƯÀÌÀû PCR primer »ç¿ë°¡´É
3'-RACE¹ý
RT ¹ÝÀÀ¶§ Oligo dT-Adaptor Primer, PCR ¹ÝÀÀ½Ã M13 M4 Primer¸¦ »ç¿ëÇÏ¿© 3'-RACE¹ý¿¡ »ç¿ë°¡´É
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ÇϳªÀÇ tube¿¡¼ ¹ÝÀÀ°¡´É. ¿Ã³¸®·Î ¿ªÀü»ç È¿¼ÒÀÇ È°¼ºÀ» ½ÇÈ°½ÃŲ ÈÄ PCR ¹ÝÀÀ
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Reverse Transcriptase XL(AMV) for RT-PCR (TaKaRa Code 2630A)
Ribonuclease Inhibitor(TaKaRa Code 2311A)
TaKaRa Ex Taq Hot Start Version
Keyword :
RT-PCR °ü·Ã Á¦Ç°
°í¼Ó¹ÝÀÀ, ³ôÀº Á¤È®µµ ½ÇÇö - 1step RT-PCR ki..
PrimeScript¢â II High Fidelity One Step RT-PCR Kit
RT-PCR kit, ²Ä²ÄÈ÷ µûÁ®º¸°í ¼±ÅÃÇϱâ
¿ªÀü»ç ¹ÝÀÀÀÇ ÃÖÀû ¿Âµµ°¡ ³ôÀº RT-PCR ¿ë Kit
BcaBEST¢ç RNA PCR Kit Ver. 1.1
PrimeScript RTase¿Í PrimeSTAR Max¸¦ Á¶ÇÕÇÏ¿©..
PrimeScript¢â High Fidelity RT-PCR Kit
Premix·Î Æí¸®ÇÑ 1 step RT-PCR kit
PrimeScript¢â One Step RT-PCR Kit Ver.2
PrimeScript RTase £¦ TaKaRa Ex Taq HS·Î ÃÖÀû..
PrimeScript¢â RT-PCR Kit
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
SMARTer¢ç RACE 5'/3' Kit
PCR±îÁö 1 tube·Î °¡´ÉÇÑ 1 step RT-PCR ¿ë Kit
TaKaRa One Step RNA PCR Kit (AMV)
Long strand cDNA ÁõÆø¿¡
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
TaKaRa Ex Taq HS°ú AMV RTase¸¦ ÀÌ¿ëÇÑ 2 step..
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0