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TaqStart Antibody

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Clontech
639250
TaqStart Antibody
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 rxns
494,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33136, x33137
Clontech
639251
TaqStart Antibody
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 rxns
921,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

TaqStart™ antibody
¸ðµç PCR ½ÇÇè¿¡ °¡Àå Æí¸®ÇÏ°í È¿°úÀûÀÎ hot start¹ý

TaqStart antibody¸¦ »ç¿ëÇϸé hot start¹ý¿¡ ÀÇÇØ PCRÀÇ Æ¯À̼º°ú °¨µµ¸¦ Çâ»ó½Ãų ¼ö ÀÖ´Ù. thermal cycleÀ» ½ÃÀÛÇϱâ Àü¿¡´Â Taq antibody¿¡ ÀÇÇØ polymerase È°¼ºÀÌ ¾ïÁ¦µÇ¹Ç·Î ºñƯÀÌÀû ÁõÆø°ú primer dimerÀÇ Çü¼ºÀÌ ¾ïÁ¦µÈ´Ù. ¿Âµµ°¡ ¿Ã¶ó°¡¸é antibody´Â Áï½Ã ºñÈ°¼ºÈ­ µÇ°í PCR ¹ÝÀÀÀÌ ÁøÇàµÈ´Ù.
TaqStart antibody´Â ´Ù¸¥ hot start¹ýº¸´Ù »ç¿ëÇϱ⠽±°í ´ÙÀ½°ú °°Àº ÀåÁ¡À» °®°í ÀÖ´Ù.
  • ´Ù¸¥ hot start È¿¼Ò¿¡¼­´Â °í¿Â¿¡¼­ incubateÇØ¾ß ÇÏ´Â °ÍÀÌ ÀÖÁö¸¸, º» antibody¿¡¼­´Â °í¿Â¿¡ ÀÇÇÑ ½Ã·áÀÇ damage¸¦ ÇÇÇÒ ¼ö ÀÖ´Ù(1~6).
  • °¡¿­ ÈÄ¿¡´Â tubeÀÇ °³Æó°¡ ºÒÇÊ¿äÇϹǷΠcross contamination À§ÇèÀ» ¹æÁöÇÒ ¼ö ÀÖ´Ù.
  • Hgh throughput PCR, in situ PCR, microplate¹ý, capillary PCR, oil free¹ý µî ´Ù¸¥ hot start¹ýÀÇ Àû¿ëÀÌ °ï¶õÇÑ PCR¿¡¼­µµ º» antibody´Â »ç¿ëÀÌ °¡´ÉÇÏ´Ù.
  • ºñƯÀÌÀû PCR »ê¹°ÀÌ ¹®Á¦°¡ µÉ °æ¿ì, ¿¹¸¦ µé¸é Ç¥Àû ¼­¿­ÀÇ copy ¼ö°¡ ÀûÀº °æ¿ì³ª ´Ù¾çÇÑ DNA¿¡ ÀÇÇÑ background, degenerate primer¸¦ »ç¿ëÇÒ °æ¿ì¿¡µµ º¸´Ù È¿°úÀûÀÎ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
TaqStart antibody´Â Taq DNA polymerase¿¡ È¿°ú°¡ ÀÖ´Â (native, recombinant, N-terminal deletion mutants)¿¡ È¿°ú°¡ ÀÖ°í Clontech»çÀÇ TITANIUM Taq DNA polymerase³ª ¸ðµç Advantage 2 Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖ´Ù. Á¦Ç°¿¡´Â dilution buffer¿Í user manual(PT1576-1)ÀÌ Æ÷ÇԵǾî ÀÖ´Ù.


±×¸² 1. TaqStart antibody´Â thermal cyclesÀÌ ½ÃÀ۵DZâÀü polymerase activity¸¦ ¾ïÁ¦ÇÑ´Ù.


±×¸² 2. TaqStart antibody¸¦ »ç¿ëÇÑ RT-PCR¿¡¼­ÀÇ ÁõÆø·®°ú ƯÀ̼º Çâ»ó
PHA-stimulated peripheral blood leukocytes ¶Ç´Â mouse RAW 264.7 macrophage ¼¼Æ÷¿¡¼­ total RNA¸¦ Á¤Á¦ÇÏ¿´´Ù. Á¤Á¦µÈ total RNA 2 §¶°ú oligo(dT) primer¸¦ »ç¿ëÇÏ¿© ¿ªÀü»ç ¹ÝÀÀÀ¸·Î cDNA¸¦ ÇÕ¼ºÇÏ¿´´Ù. cDNA ÁÖÇü°ú Taq DNA polymerase¸¦ »ç¿ëÇÏ¿© TaqStart ¸¦ ³ÖÀº ¹ÝÀÀ(+)°ú TaqStart¸¦ ³ÖÁö ¾ÊÀº ¹ÝÀÀ(-)À¸·Î 35 cycle ÁõÆøÀ» ½Ç½ÃÇÏ¿´´Ù. ±× °á°ú PCR »ê¹°À» 1.8 % agarose/EtBr gel·Î Àü±â¿µµ¿ÇÏ¿´´Ù. Lane 1 : human c-jun(410 bp). Lane 2 : mouse IL-2 receptor(700 bp). Lane 3 : mouse inducible nitric oxide synthase(497 bp). Lane M : ©ªX174/Hae¥² DNA size marker
º¸Á¸ -20¡É
Àû¿ëºÐ¾ß
- Hot start PCR
- Quantitative PCR
- Multiplex PCR
- Genotyping
Âü°í¹®Çå
1. Fromenty, B., et al.(2000) Nucleic Acids Res. 28:11.
2. Barnes, W.M.(1994) Proc
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