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> TaqStart Antibody
TaqStart Antibody
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Clontech
639250
TaqStart Antibody
200 rxns
494,000¿ø
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Clontech
639251
TaqStart Antibody
500 rxns
921,000¿ø
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Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
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HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
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High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
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Clontech PCR-Select Ba..
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TaKaRa PCR Thermal Cyc..
PCR Accessory Products
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TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
TaqStart™ antibody
¸ðµç PCR ½ÇÇè¿¡ °¡Àå Æí¸®ÇÏ°í È¿°úÀûÀÎ hot start¹ý
TaqStart antibody¸¦ »ç¿ëÇϸé hot start¹ý¿¡ ÀÇÇØ PCRÀÇ Æ¯À̼º°ú °¨µµ¸¦ Çâ»ó½Ãų ¼ö ÀÖ´Ù. thermal cycleÀ» ½ÃÀÛÇϱâ Àü¿¡´Â Taq antibody¿¡ ÀÇÇØ polymerase È°¼ºÀÌ ¾ïÁ¦µÇ¹Ç·Î ºñƯÀÌÀû ÁõÆø°ú primer dimerÀÇ Çü¼ºÀÌ ¾ïÁ¦µÈ´Ù. ¿Âµµ°¡ ¿Ã¶ó°¡¸é antibody´Â Áï½Ã ºñÈ°¼ºÈ µÇ°í PCR ¹ÝÀÀÀÌ ÁøÇàµÈ´Ù.
TaqStart antibody´Â ´Ù¸¥ hot start¹ýº¸´Ù »ç¿ëÇϱ⠽±°í ´ÙÀ½°ú °°Àº ÀåÁ¡À» °®°í ÀÖ´Ù.
´Ù¸¥ hot start È¿¼Ò¿¡¼´Â °í¿Â¿¡¼ incubateÇØ¾ß ÇÏ´Â °ÍÀÌ ÀÖÁö¸¸, º» antibody¿¡¼´Â °í¿Â¿¡ ÀÇÇÑ ½Ã·áÀÇ damage¸¦ ÇÇÇÒ ¼ö ÀÖ´Ù(1~6).
°¡¿ ÈÄ¿¡´Â tubeÀÇ °³Æó°¡ ºÒÇÊ¿äÇϹǷΠcross contamination À§ÇèÀ» ¹æÁöÇÒ ¼ö ÀÖ´Ù.
Hgh throughput PCR, in situ PCR, microplate¹ý, capillary PCR, oil free¹ý µî ´Ù¸¥ hot start¹ýÀÇ Àû¿ëÀÌ °ï¶õÇÑ PCR¿¡¼µµ º» antibody´Â »ç¿ëÀÌ °¡´ÉÇÏ´Ù.
ºñƯÀÌÀû PCR »ê¹°ÀÌ ¹®Á¦°¡ µÉ °æ¿ì, ¿¹¸¦ µé¸é Ç¥Àû ¼¿ÀÇ copy ¼ö°¡ ÀûÀº °æ¿ì³ª ´Ù¾çÇÑ DNA¿¡ ÀÇÇÑ background, degenerate primer¸¦ »ç¿ëÇÒ °æ¿ì¿¡µµ º¸´Ù È¿°úÀûÀÎ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
TaqStart antibody´Â Taq DNA polymerase¿¡ È¿°ú°¡ ÀÖ´Â (native, recombinant, N-terminal deletion mutants)¿¡ È¿°ú°¡ ÀÖ°í Clontech»çÀÇ TITANIUM Taq DNA polymerase³ª ¸ðµç Advantage 2 Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖ´Ù. Á¦Ç°¿¡´Â dilution buffer¿Í user manual(PT1576-1)ÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
±×¸² 1. TaqStart antibody´Â thermal cyclesÀÌ ½ÃÀ۵DZâÀü polymerase activity¸¦ ¾ïÁ¦ÇÑ´Ù.
±×¸² 2. TaqStart antibody¸¦ »ç¿ëÇÑ RT-PCR¿¡¼ÀÇ ÁõÆø·®°ú ƯÀ̼º Çâ»ó
PHA-stimulated peripheral blood leukocytes ¶Ç´Â mouse RAW 264.7 macrophage ¼¼Æ÷¿¡¼ total RNA¸¦ Á¤Á¦ÇÏ¿´´Ù. Á¤Á¦µÈ total RNA 2 §¶°ú oligo(dT) primer¸¦ »ç¿ëÇÏ¿© ¿ªÀü»ç ¹ÝÀÀÀ¸·Î cDNA¸¦ ÇÕ¼ºÇÏ¿´´Ù. cDNA ÁÖÇü°ú Taq DNA polymerase¸¦ »ç¿ëÇÏ¿© TaqStart ¸¦ ³ÖÀº ¹ÝÀÀ(+)°ú TaqStart¸¦ ³ÖÁö ¾ÊÀº ¹ÝÀÀ(-)À¸·Î 35 cycle ÁõÆøÀ» ½Ç½ÃÇÏ¿´´Ù. ±× °á°ú PCR »ê¹°À» 1.8 % agarose/EtBr gel·Î Àü±â¿µµ¿ÇÏ¿´´Ù. Lane 1 : human c-jun(410 bp). Lane 2 : mouse IL-2 receptor(700 bp). Lane 3 : mouse inducible nitric oxide synthase(497 bp). Lane M : ©ªX174/Hae¥² DNA size marker
º¸Á¸ -20¡É
Àû¿ëºÐ¾ß
- Hot start PCR
- Quantitative PCR
- Multiplex PCR
- Genotyping
Âü°í¹®Çå
1. Fromenty, B., et al.(2000) Nucleic Acids Res. 28:11.
2. Barnes, W.M.(1994) Proc
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639250
639251
Keyword :
PCR Accessory Products
Advantage Ultrapure PCR Deoxynucleotide Mix
TaqStart Antibody