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Fungal rDNA (ITS1) PCR Kit Fast

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Takara
RR183A
Fungal rDNA (ITS1) PCR Kit Fast
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 tests
452,800¿ø 
566,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR183A_e.v2203Da.pdf

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Fungal rDNA (ITS1) PCR Kit Fast(Code RR183A)´Â Áø±Õ·ùÀÇ ribosomal DNA(rDNA)¿µ¿ª ³»ÀÇ ITS1¿µ¿ª(150~500 bp)À» PCRÁõÆø ÇÒ ¼ö ÀÖ´Â ½Ã¾à°ú sequencing primer·Î ±¸¼ºµÇ¾î ÀÖ´Ù. º» Á¦Ç°¿¡ Á¦°øµÇ´Â primer¸¦ ÀÌ¿ëÇÑ ºÐ¼®°úÁ¤Àº 16Â÷ °³Á¤µÈ Japanese PharmacopoeiaÀÇ ¡°Rapid Identification of Microorganisms Using Genetic Analysis¡± ºÎºÐ¿¡ ¼ö·ÏµÇ¾î ÀÖ´Ù.

º» Á¦Ç°µé·Î ÁõÆøµÈ PCR »ê¹°Àº sequencing ºÐ¼®À» ÅëÇØ ¾òÀº ¿°±â¼­¿­°ú µ¥ÀÌÅͺ£À̽º ³» ¼­¿­°úÀÇ »óµ¿¼º °Ë»ç¸¦ ÅëÇØ ±ÕÀ» ºÐ·ùÇÒ ¼ö ÀÖ´Ù. °³·®Çü Taq DNA polymerase¸¦ »ç¿ëÇÏ°í ÀÖ¾î, °í¼Ó PCR Á¶°ÇÀ¸·Î ½Å¼ÓÇÏ°Ô PCRÁõÆøÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ÀÌ È¿¼Ò´Â Ç×ü¸¦ ÀÌ¿ëÇÑ hot-start PCR È¿¼Ò·Î cycleÀü mis-primingÀ̳ª primer-dimer¿¡ À¯·¡ÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù.

[ÁÖÀÇ] ÀÌ Á¦Ç°µé·Î Áø±Õµé Áß ÀϺδ PCR ÁõÆøÀÌ ¾È µÇ´Â °æ¿ìµµ ÀÖ´Ù.
¼¼±Õ ºÐ·ù¸¦ À§Çؼ­´Â Bacterial 16S rDNA PCR Kit (Code RR180A) ¶Ç´Â Bacterial 16S rDNA PCR Kit Fast (800) (Code RR182A)¸¦ »ç¿ëÇϱ⠹ٶõ´Ù.
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Fungal rDNA (ITS1) PCR Kit Fast (Code RR183A), (25 ¥ìl PCR¹ÝÀÀ, 50ȸ)

1.

TaKaRa Taq HS Fast Detect Premix (2x)

625 ¥ìl

2.

rDNA ITS1 Primer Mix (10x)*1

125 ¥ìl

3.

dH2O

650 ¥ìl

4.

Positive Control (S. cerevisiae DNA) (1 ng/¥ìl)

25 ¥ìl

5.

Sequencing Primer ITS1 F (7.5 pmol/¥ìl)

50 ¥ìl

6.

Sequencing Primer ITS1 R (7.5 pmol/¥ìl)

50 ¥ìl


£ª1 rDNA ITS1 Mix¿¡ Æ÷ÇÔµÈ primer´Â Sequencing Primer ITS1 F°ú Sequencing Primer ITS1 R°ú °°Àº ¼­¿­ÀÌ´Ù.

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TaKaRa Taq HS Fast Detect Premix¿¡´Â PCRÈ¿¼Ò°¡ Æ÷ÇԵǾî Àֱ⠶§¹®¿¡ °úµµÇÑ µ¿°á À¶Çظ¦ ¹Ýº¹Çϸé È°¼ºÀÌ ¶³¾îÁö´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
³ìÀÏ ¶§´Â °Ý·ÄÇÑ vortexingÀ» ÇÇÇÏ°í À¶ÇØ ÈÄ¿¡´Â ºÎµå·´°Ô Àß ¼¯¾î ÁֽʽÿÀ.

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