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Home > ÀüÁ¦Ç°º¸±â > PCR > Ư¼ö PCR (Epigenetics, UNG µî) > BcaBEST¢ç DNA Polymerase ver.2.0
ÇÙ»êµî¿ÂÁõÆø¿ë PCR È¿¼Ò

BcaBEST¢ç DNA Polymerase ver.2.0

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR380A
BcaBEST¢ç DNA Polymerase ver.2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,600 U
94,400¿ø 
118,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR380A_DS.v2211Da.pdf
Takara
RR380B
BcaBEST¢ç DNA Polymerase ver.2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
8,000 U (RR380A x 5)
530,000¿ø 

  • LAMP (Loop-Mediated Isothermal Amplification)¹ý¿¡ »ç¿ë °¡´ÉÇÑ ÁßÇÕ È¿¼Ò
  • Çü±¤ »ö¼Ò¿Í Real Time PCR ÀåÄ¡¸¦ ÀÌ¿ëÇÏ¿© Real-Time LAMP/Real-Time RT-LAMP °¡´É
  • Áö½Ã¾à°ú »ö¼Ò¸¦ ÀÌ¿ëÇÑ °ËÃâÀ̳ª ¸é¿ª Å©·Î¸¶Åä ¹ý (lateral-flow assay)°ú Á¶ÇÕ °¡´É
Á¦Ç°¼³¸í
BcaBEST¢ç DNA Polymerase ver.2.0´Â Bacillus caldotenax À¯·¡·Î 5'→ 3' exonuclease È°¼ºÀÌ °á¼ÕµÈ DNAÁßÇÕ È¿¼Ò (BcaBEST¢ç DNA Polymerase)ÀÇ ¾÷±×·¹À̵å Á¦Ç°ÀÌ´Ù. ±âÁ¸ Á¦Ç°ÀÇ °­·ÂÇÑ strand-displacement È°¼ºÀº À¯ÁöÇϸç DNA ÁßÇÕÈ¿¼ÒÀÇ ¼º´ÉÀ» Çâ»ó½ÃÄÑ ¼Óµµ¿Í °¨µµ°¡ ³ô¾ÆÁ®, º» È¿¼Ò·Î ÇÙ»ê µî¿Â ÁõÆøÀ» ÁøÇàÇÏ¸é ±âÁ¸ º¸´Ù ´Ü½Ã°£¿¡ ¹Ì·® ÇÙ»êÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù. º» Á¦Ç°¿¡´Â dNTP¸¦ Æ÷ÇÔÇÑ LAMP (Loop-Mediated Isothermal Amplification)¿ë ¹ÝÀÀ ¹öÆÛ°¡ Æ÷ÇԵǾî Ç¥Àû À¯ÀüÀÚÀÇ °ËÃ⠽ýºÅÛ ±¸Ãà¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
±¸¼º

1.

BcaBEST¢ç DNA Polymerase ver.2.0

200 §¡ ×1

2.

2× BcaBEST¢ç Buffer

1.25 §¢ ×2

º¸Á¸ -20¡É
³óµµ 8 U/§¡
±â¿ø
Escherichia coli carrying a plasmid containing the gene for BcaBEST¢ç DNA Polymerase ver.2.0
ÀϹÝÀû ¼ºÁú Áú·®: ¾à 68 kDa
È°¼ºÀÇ Á¤ÀÇ
65¡É, 30ºÐ°£ 210 nmolÀÇ dNTP¸¦ »ê ºÒ¿ë¼º ħÀü¹°·Î »ý¼ºÇÏ´Â È¿¼Ò ¾çÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

25 mM

 

TAPS (pH9.3, 25¡É)

50 mM

 

KCl

2 mM

 

MgCl2

0.1 mM

 

DTT

°¢ 200μ M

 

dATP, dGTP, dCTP

100 µM

 

dTTP

10 µCi

 

[3H]-dTTP

0.25 mg/§¢

 

È°¼ºÈ­ ¿¬¾î Á¤ÀÚ DNA

¿ëµµ
  • LAMP (Loop-Mediated Isothermal Amplification)/ RT-LAMP (Reverse Transcription LAMP)
  • Real-Time LAMP/Real-Time RT-LAMP
  • Colorimetric LAMP Assay
  • Immunochromatography (lateral-flow assay)

Keyword : LAMP,ÇÙ»êµî¿ÂÁõÆø,POCT

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