´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > Terra PCR Direct Polymerase Mix
Direct PCR È¿¼Ò

Terra PCR Direct Polymerase Mix

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
639270
Terra PCR Direct Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
200rxns
294,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33142, x33143
Clontech
639271
Terra PCR Direct Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 rxns (639270 x 4)
1,059,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639285
Terra PCR Direct Genotyping Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200rxns
791,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33141
Clontech
639286
Terra PCR Direct Red Dye Premix
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 rxns
400,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33144

2013³â 10¿ù ¾÷µ¥ÀÌÆ® : Terra PCR Direct Polymerase Mix (code 639269, 639270, 639271)°ú Terra PCR Direct Red Dye Premix (code 639286)ÀÇ Á¦Ç° ±¸¼ºÇ°¿¡ Proteinase K°¡ Ãß°¡ µÇ¾ú½À´Ï´Ù. ÀÚ¼¼ÇÑ ³»¿ëÀº ¸Å´º¾óÀ» ÂüÁ¶Çϼ¼¿ä.
Terra PCR Direct Polymerase¸¦ ÀÌ¿ëÇÑ °£ÆíÇÑ genotyping
  • ½Ã·áÀÇ prep °úÁ¤ »ý·«
  • µ¿¹°/½Ä¹°ÀÇ Á¶Á÷À¸·ÎºÎÅÍ Á÷Á¢ PCR °¡´É
  • GC-rich Ÿ°Ù À¯ÀüÀÚÀÇ ÁõÆø¿¡µµ ÀûÇÕ
  • Hot start È¿¼Ò·Î ½Ç¿Â¿¡¼­µµ ¹ÝÀÀ ¼Â¾÷ °¡´É

Terra PCR Direct Polymerase MixÀ» »ç¿ëÇϸé DNA ÃßÃâ°ú Á¤Á¦ °úÁ¤ ¾øÀÌ Ç÷¾×, µ¿¡¤½Ä¹° Á¶Á÷À̳ª ÆĶóÇÉ ÀýÆí Á¶Á÷ µîÀ¸·ÎºÎÅÍ °ð¹Ù·Î PCR ÁõÆø ¹ÝÀÀÀ» ÁøÇàÇÒ ¼ö Àֱ⠶§¹®¿¡ ¿¬±¸ÀÚµéÀÇ ±ÍÁßÇÑ ½Ã°£°ú ¿¬±¸ºñ¸¦ Àý¾àÇÒ ¼ö ÀÖ´Ù. º» È¿¼Ò´Â 2 kb±îÁö ¸ñÀû DNA¸¦ ÁõÆøÇÒ ¼ö ÀÖÀ¸¸ç 70% ÀÌ»óÀÇ GC ÇÔ·®ÀÌ ³ôÀº À¯ÀüÀÚ ÁõÆø¿¡µµ ¸Å¿ì À¯¿ëÇÏ´Ù. ¶ÇÇÑ ¸Å¿ì ³ôÀº ¹Î°¨µµ¸¦ °®°í Àֱ⠶§¹®¿¡ ±Ø¼Ò·®¸¸ ȹµæÇÒ ¼ö ÀÖ´Â ±ÍÁßÇÑ ½Ã·áÀÇ ÁõÆø¿¡µµ ÀûÇÕÇÏ´Ù.
Clontech¿¡¼­´Â ±âº»ÀûÀÎ È¿¼Ò »Ó¸¸ ¾Æ´Ï¶ó red dye°¡ Æ÷ÇԵǰųª FFPE ½Ã·á³ª FTA card·ÎºÎÅÍ ÁõÆøÇÒ ¼ö ÀÖ´Â ´Ù¾çÇÑ Terra PCR Direct Polymerase Mix ½Ã¸®Á º¸À¯ÇÏ°í ÀÖ´Ù

Terra PCR Direct´Â Ç÷¾×, ²¿¸®³ª ±Í¿Í °°Àº »ý°Ë½Ã·á³ª ½Ä¹°ÀÇ ÀÙ µîÀ¸·ÎºÎÅÍ direct PCRÇϵµ·Ï ÃÖÀûÈ­µÇ¾î ÀÖ´Ù. À̶§ µ¿¹° Á¶Á÷À¸·ÎºÎÅÍ °ð¹Ù·Î ÁõÆøÇÑ PCR product´Â agarose gel¿¡ ºÐ¼®µÇÁö ¾Ê´Â °æ¿ì°¡ Àִµ¥ À̶§ gel loading buffer¿¡ Proteinase K¸¦ ÷°¡Çؼ­ ·ÎµùÀ» Çϸé DNA°¡ well¿¡ °É¸®Áö ¾Ê¾Æ Àü±â¿µµ¿À¸·Î ½±°Ô ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. µû¶ó¼­ ¸¸¾à µ¿¹° Á¶Á÷À¸·ÎºÎÅÍ direct PCRÀ» ÇÏ°íÀÚ ÇÒ ¶§ Terra PCR Direct Polymerase Mix¿Í ÇÔ²² Proteinase K¸¦ ÷°¡ÇÏ´Â °ÍÀ» ÃßõÇÑ´Ù.
Terra PCR Direct Polymerase Àû¿ë »ç·Ê
¸¶¿ì½º, Àΰ£, »õÅÐ, C.elegans, °³±¸¸®(Xenopus), È¿¸ð/±Õ»çü, Æí¸ðÃæ(Leishmania), ¹è¾ç¼¼Æ÷, ¾Ö±âÀå´ë, ¹Ð, ½Ò, »çÅÁ¼ö¼ö, ¹ã³ª¹«, À̳¢ Æ÷ÀÚü, ÀáÀÚ¸®,ÃÊÆĸ®, ¸»¹ú

Á¦Ç°¼³¸í
Terra PCR Direct has been optimized for direct PCR amplification from animal and plant tissues, such as blood (Figure 1), tail and ear biopsies (Figure 2), or leaf cuttings (Figure 3). Importantly, PCR products amplified directly from animal tissues do not always resolve well on agarose gels (Figure 2, Lane 1); however, this can be easily prevented by adding proteinase K to your gel loading buffer (Figure 2, Lane 2). Consequently, we recommend ordering Proteinase K along with Terra PCR Direct Polymerase Mix if you are amplifying directly from animal tissues.



Applications
Perform direct PCR amplification from tissue samples, crude extracts, and dirty templates without having to extract and purify the DNA.
Components
- Terra PCR Direct Polymerase Mix (1.25 U/¥ìl)
- 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
º¸Á¸ -20¡É


Terra PCR Direct Solution

Polymerase Mix

Red Dye Premix

Genotyping Kit

FFPE Kit

FTA Card Kit

Features:

General purpose; suitable for most applications

Premix with convenient loading

All-in-one kit; most robust performance

Application-specific kit

Application-specific kit

Components:

Buffer
[Mg2+, dNTPs]

Premix
[Mg2+, dNTPs,
 
Polymerase Mix,
 
Loading Dye]
 

Buffer
[Mg2+, dNTPs]

Buffer
[Mg2+, dNTPs]

Buffer
[Mg2+, dNTPs]

Polymerase Mix

Polymerase Mix

Polymerase Mix

Polymerase Mix

 

Loading Dye

 

 

 

 

Extraction Buffer*

Extraction Buffer

 

Proteinase K

Proteinase K

Proteinase K

Proteinase K

 

Recommended Sample Types:

 

FFPE

 

 

O

 

Blood (FTA)

 

 

 

O

Blood (Heparin)

O

O

 

 

Plant Leaves (FTA)

 

 

 

O

Plant leaves

O

 O*

 

 

Buccal cells (FTA)

 

 

 

O

Buccal cells

O

O

 

 

Saliva

O

O

 

 

Tissues

O

 O*

 

 

Cell pellet

O

O

 

 

Crude extract

O

O

 

 

Purified DNA

O

O

 

 

* The Terra PCR Direct Genotyping Kit includes an extraction buffer, which we recommended using tough plants or tissue samples.


Keyword : directpcr,direct pcr,pcr,gcrich,hoststart,mouse,blood,½Ä¹°,º­,FTA

PCR Enzyme (Clontech)
>
ClontechÀÇ µ¶ÀÚÀûÀÎ blend Çü È¿¼Ò
Advantage 2 Polymerase Mix & PCR Kit
>
Super accuracy, high specificity
Advantage HD Polymerase Mix
>
High Fidelity PCR È¿¼Ò·Î Cloning¿¡ ÃÖÀû
CloneAmp HiFi PCR Premix
>
³ôÀº fidelity¿Í ÁõÆøÈ¿À²À» °âºñÇÑ µ¿°á°ÇÁ¶µÈ..
High Fidelity PCR EcoDry Premix
>
³ôÀº ÁõÆø È¿À²ÀÇ µ¿°á°ÇÁ¶µÈ PCR mastermix
High Yield PCR EcoDry Premix
>
ºü¸£°í ±ÕÀÏÇÑ Multiplexing PCRÀ» À§ÇÑ 2X PCR..
SuperPlex¢â Premix
>
Direct PCR È¿¼Ò
Terra PCR Direct Polymerase Mix
>
5£§-exonuclease È°¼ºÀ» Á¦°ÅÇÑ PCR È¿¼Ò
TITANIUM¢ç Taq DNA Polymerase