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TaKaRa
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Å°¿öµå°Ë»ö
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  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard

TaKaRa Ex Taq

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR001A
TaKaRa Ex Taq¢ç
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
120,800¿ø 
151,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR001A_DS.v2110Da.pdf
Takara
RR001B
TaKaRa Ex Taq¢ç
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U
432,000¿ø 
540,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR001B_DS.v2110Da.pdf
Takara
RR01AM
(RR001AM)
TaKaRa Ex Taq¢ç (Mg2 free Buffer)
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
120,800¿ø 
151,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR01AM_DS.v2110Da.pdf
Takara
RR01BM
(RR001BM)
TaKaRa Ex Taq¢ç (Mg2 free Buffer)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (RR01AM x 4)
536,000¿ø 


Á¦Ç° ¼³¸í
º» Á¦Ç°Àº TaKaRa Taq¢âÀÇ ÀÔÁõµÈ ¼º´É°ú È¿À²ÀûÀÎ 3¡¯ -> 5¡¯ exonucleaseÀÇ ±³Á¤´É·ÂÀ» ¸ðµÎ °®Ãá °íÈ¿À², °í°¨µµÀÇ DNA polymeraseÀÌ´Ù. ±æÀÌ°¡ ±ä ´ÜÆíÀÇ ÁõÆø¿¡µµ ÀûÇÕÇϸç ÀϹÝÀûÀÎ PCR application¿¡¼­ ±âÁ¸ÀÇ Taq DNA polymeraseº¸´Ù ³ôÀº ÁõÆøÈ¿À², ³·Àº mismatchÀ²À» ½ÇÇöÇÏ¿´´Ù.
TaKaRa Ex Taq¢çÀº ¹ÝÀÀ¿ë buffer (Mg2+ Æ÷ÇÔ ¶Ç´Â Mg2+ º°µµÁ¦°ø) ¿Í dNTP mixture°¡ ÇÔ²² Á¦°øµÈ´Ù.
³»¿ë (250 U)
TaKaRa Ex Taq¢ç (Code RR001A)
1. TaKaRa Ex Taq (5 U/§¡) 250 U
2. 10¡¿ Ex Taq Buffer (20 mM Mg2+ plus) 1 §¢
3. dNTP Mixture (°¢ 2.5 mM) 800 §¡

TaKaRa Ex Taq¢ç(Mg2+ free Buffer) (Code RR001AM)
1. TaKaRa Ex Taq (5 U/§¡) 250 U
2. 10¡¿ Ex Taq Buffer (Mg2+ free) 1 §¢
3. MgCl2(25 mM) 1 §¢
4. dNTP Mixture (°¢ 2.5 mM) 800 §¡
º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl (pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

NP-40

50%

Glycerol

÷ºÎ dNTP Mixture Á¶¼º*

³óµµ

°¢ 2.5 mM

Çü»ó

¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9

¼øµµ

°¢ 98% ÀÌ»ó

*dATP, dCTP, dGTP, dTTPµî ¸ô È¥ÇÕ¹°·Î Èñ¼®ÇÏÁö ¾Ê°í ±×´ë·Î PCR¹ÝÀÀ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇÏ¿©
TaKaRa Ex Taq¢çÀ» ÀÌ¿ëÇÏ¿© ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3¡¯ ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector pMD20 (Code 3270) ¶Ç´Â T-vector pMD19 (Simple) (Code 3271)¿¡ cloningÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸»´Ü ÆòÈ°È­ ¹× ÀλêÈ­ ÈÄ, ÆòÈ° ¸»´Ü vector¿¡ cloningÇÒ ¼öµµ ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö: TaKaRa Ex Taq¢ç Hot Start Version
- 2X PCR master mix: Premix Taq¢â (Ex Taq Version 2.0) (500 ¥ìl¡¿6 tube)
- ¿ªÀü»ç¹ÝÀÀÀ» À§ÇÑ all-in-One kit: PrimeScript¢â 1st strand cDNA Synthesis Kit
- 5x premix ¿ªÀü»ç ½Ã¾à: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword : rr001,extaq,RR001AM,RR001BM,¹éÀü¹é½ÂPCR,¹éÀü¹é½Â PCR

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)