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Home > ÀüÁ¦Ç°º¸±â > PCR > Direct PCR: MightyAmp > °­ÇÑ ÁõÆø´É·Â- Ver.3
°­·ÂÇÏ°Ô ÁøÈ­ÇÑ direct PCR¿ë È¿¼Ò

°­ÇÑ ÁõÆø´É·Â- Ver.3

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R076A
MightyAmp¢â DNA Polymerase Ver.3
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
250 U
237,600¿ø 
297,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R076A_e.v1805Da.pdf
Takara
R076B
MightyAmp¢â DNA Polymerase Ver.3
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (R076A x 4)
1,068,000¿ø 



  • ´Ù¾çÇÑ PCR inhibitor (humic acid, NaClµîÀÇ salt, tannic acid µî)¿¡ ´ëÇÑ ÀúÇ×¼º Çâ»ó
  • FTA card, EDTA blood, heparin bloodÀÇ direct PCR¿¡µµ ÃÖÀû (½ÇÇè ¿¹ Âü°í)
  • ºñƯÀÌÀû ÁõÆøÀ̳ª smear¸¦ ¾ïÁ¦ÇÏ´Â Additive ½Ã¾à Æ÷ÇÔ
Á¦Ç°¼³¸í
MightyAmp DNA Polymerase´Â ³ôÀº ¹ÝÀÀ¼ºÀ» °¡Áö´Â ½Å±Ô DNA polymerase·Î, °­·ÂÇÑ ÁõÆø ´É·Â¿¡ ÀÇÇØ ÀϹÝÀûÀÎ PCR È¿¼Ò¿¡¼­ ÁõÆøÀÌ ¾î·Á¿î PCR ÀúÇØ ¹°ÁúÀ» ¸¹ÀÌ Æ÷ÇÔÇÏ´Â crude »ýü ÃßÃâ¾×À¸·ÎºÎÅÍ PCR ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
MightyAmp DNA Polymerase Ver. 3´Â MightyAmp DNA Polymerase¸¦ °³·®ÇÑ PCR È¿¼Ò¿Í »õ·Î¿î Á¶¼ºÀÇ buffer°¡ Á¶ÇÕµÈ Á¦Ç°À¸·Î Ver. 2¿¡ ºñÇØ PCR ¾ïÁ¦¹°Áú¿¡ ´ëÇÑ ÀúÇ×¼ºÀÌ Áõ°¡ÇÏ¿´´Ù.
¶ÇÇÑ, Ç÷¾×, µ¿½Ä¹° Á¶Á÷ µîÀÇ »ýü ½Ã·á¸¦ Á÷Á¢ ¹ÝÀÀ¾×¿¡ ÷°¡ÇÏ´Â ¡¸Direct PCR¡¹ ¼º´Éµµ Çâ»óµÇ¾ú´Ù.
GC rich, AT rich ¼­¿­¿¡ °ü°è¾øÀÌ, ±¤¹üÀ§ÇÑ Å¸°ÙÀÇ ÁõÆøÀÌ °¡´ÉÇϸç, ÇÊ¿ä¿¡ µû¶ó Á¦Ç°³» Æ÷ÇԵǾî ÀÖ´Â 10¡¿Additive for High Specificity¸¦ PCR ¹ÝÀÀ¿ë¾×¿¡ ÷°¡ÇÔÀ¸·Î ½á ÁõÆø ƯÀ̼ºÀ» Áõ°¡ ½Ãų ¼ö ÀÖ´Ù.
¶ÇÇÑ 98 ¡É±îÁö polymerase È°¼ºÀ» ¾ïÁ¦ÇÏ´Â °­·ÂÇÑ monoclonal Ç×ü¸¦ ÀÌ¿ëÇÏ¿© hot start PCRÀÌ °¡´ÉÇÏ´Ù.
³»¿ë
MightyAmp DNA Polymerase Ver.3 (R076A, 250 U; 200 ȸ¿ë*1)
1.

MightyAmp DNA Polymerase Ver.3

200 §¡

2.

2¡¿MightyAmp Buffer Ver.3 (Mg2£«, dNTP plus)£ª2

1 §¢ x 5

3.

10¡¿Additive for High Specificity

500 §¡ x 2

*1 50§¡ PCR ¹ÝÀÀ½Ã ¿ë·®
*2 Mg2+ 4mM (2x), dNTP 600 ¥ìM each (2x) Æ÷ÇÔ
º¸Á¸ -20 ¡É
ÁÖÀÇ
  • 10¡¿Additive for High Specificity¸¦ ÷°¡ÇÏÁö ¾Ê´Â ¹ÝÀÀÀ» 1Â÷ ÃßõÇϸç,
  • Áö¼ÓÀûÀ¸·Î ºñƯÀÌÀû ÁõÆø »ê¹° ¶Ç´Â smear ÆÐÅÏÀÌ ³ªÅ¸³ª ÁõÆøƯÀ̼º ³ôÀÌ°íÀÚ ÇÒ ¶§ 10¡¿Additive for High Specificity¸¦ ÷°¡ÇÏ´Â °ÍÀ» ÃßõÇÔ.
  • µ¿¹° Á¶Á÷¿¡¼­ direct PCR»ê¹°À» Àü±â¿µµ¿ ÇÒ °æ¿ì Loading Buffer¿¡ Proteinase K (Code 9034)À» ÷°¡ÇÒ °Í.
½ÇÇè ¿¹
1. Ç÷¾× ¹× FTAÄ«µåÀÇ direct PCR
±âÁ¸ Á¦Ç° (Ver.2)°ú ºñ±³ÇÏ¿© Ver.3¿¡¼­´Â Ç÷¾×, µ¿/½Ä¹° Á¶Á÷ µî »ýü ½Ã·áÀÇ direct PCR ¼º´ÉÀÌ Çâ»óµÇ¾ú´Ù. ¶ÇÇÑ ÀϹÝÀûÀ¸·Î DNA Á¤Á¦°¡ ÇÊ¿äÇÑ Ç÷¾×, ±¸°­ Á¡¸·, ½Ä¹°ÀÇ ÀÙ µîÀ» µµÆ÷ÇÑ FTAÄ«µå, NucleoCard¢ç, Á¾ÀÌÀÇ direct PCRµµ °¡´ÉÇÏ´Ù.

ÁÖÇü:

Human EDTA ó¸® Ç÷¾×
¶Ç´Â Heparin ó¸® Ç÷¾× (5 §¡/25 §¡ ¹ÝÀÀ¾×)

Ÿ°Ù:

DCLRE1A 1 kb

 

1:

MightyAmp Ver.2 (±âÁ¸ Á¦Ç°)

2:

MightyAmp Ver.3 Additive (-)

3:

MightyAmp Ver.3 Additive (+)

4:

Takara Taq °è¿­ È¿¼Ò

ÁÖÇü:

Áö¸§ 1.2 mmÀÇ ÆÝÄ¡·Î ÀÚ¸¥
Mouse Blood FTA Card/ 25 §¡ ¹ÝÀÀ¾×

Ÿ°Ù:

Hbb-b1 542 bp

5:

A»ç È¿¼Ò

6:

B»ç PCR ÀúÇØ ¹°Áú ³»¼º È¿¼Ò

M1:

DL2,000 DNA Marker

M2:

100 bp DNA Ladder


2. °í³óµµÀÇ humic acid¸¦ Æ÷ÇÔÇÏ´Â »ùÇÃÀÇ PCR
PCR ¹ÝÀÀÀ» °­ÇÏ°Ô ÀúÇØÇÏ´Â °ÍÀ¸·Î ¾Ë·ÁÁø humic acid°¡ °í³óµµ·Î Æ÷ÇÔµÈ »ùÇÃÀÇ PCR °á°ú, Ÿ»ç Á¦Ç° ´ëºñ ÁõÆøÈ¿À²ÀÌ ¸Å¿ì ³ô¾Ò´Ù.


ÁÖÇü:

E. coli genomic DNA (2 x 105 copies¿¡ »ó´ç)

1:

Humic acid

0 §¶

Ÿ°Ù:

16S rDNA (173 bp)

2:

 

0.1 §¶

 

 

3:

 

0.2 §¶

 

 

4:

 

0.3 §¶

 

 

5:

 

0.4 §¶ / 25§¡ ¹ÝÀÀ¾×

 

 

M:

100 bp DNA Ladder

°ü·ÃÁ¦Ç°
- ¹ü¿ëÀûÀÎ ¿Ã¶ó¿î´õ agarose: SeaKem LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword : direct PCR,Á÷Á¢PCR,humic acid,FTAÄ«µå,´ÙÀÌ·ºÆ®PCR

Direct PCR: MightyAmp
>
°­·ÂÇÏ°Ô ÁøÈ­ÇÑ direct PCR¿ë È¿¼Ò
°­ÇÑ ÁõÆø´É·Â- Ver.3
>
DNA °£ÀÌÃßÃâºÎÅÍ genotyping±îÁö ÇϳªÀÇ kit·Î
Genotyping¿ë ÃßÃâ½Ã¾àÆ÷ÇÔ
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ÃßÃâ Á¶ÀÛÀÌ ÇÊ¿ä¾ø´Â direct PCRÀÌ °¡´É!!
񃧯-Ver.2