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Home > ÀüÁ¦Ç°º¸±â > PCR > RT-PCR °ü·Ã Á¦Ç° > PrimeScript¢â High Fidelity RT-PCR Kit
PrimeScript RTase¿Í PrimeSTAR Max¸¦ Á¶ÇÕÇÏ¿© ÃÖ°íÀÇ Á¤È®µµ¸¦ °¡Áø PCR °¡´É

PrimeScript¢â High Fidelity RT-PCR Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R022A
PrimeScript¢â High Fidelity RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
50 ȸ
308,000¿ø 
385,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R022A_e.v2107Da.pdf
Takara
R022B
PrimeScript¢â High Fidelity RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ (R022A x 4)
1,388,000¿ø 

Á¦Ç° ¼³¸í
PrimeScript High Fidelity RT-PCR KitÀº total RNA ȤÀº mRNA·ÎºÎÅÍ ¸Å¿ì Á¤È®ÇÏ°Ô cDNA¸¦ ÇÕ¼ºÇϱâ À§ÇÑ RT-PCR¿ë Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ¿ªÀü»ç ¹ÝÀÀ¿¡´Â M-MLV À¯·¡ÀÇ RTase¸¦ ±âÃÊ·Î ´ÙÄ«¶ó¹ÙÀÌ¿À°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ¿ªÀü»çÈ¿¼Ò PrimeScript RTase¸¦, PCR¿¡´Â ÃÖ°í Á¤È®µµÀÇ High Fidelity PCR È¿¼ÒÀÎ PrimeSTAR Max DNA Polymerase¸¦ ÀÌ¿ëÇÏ°í ÀÖ´Ù. PrimeSTAR Max´Â ÃÖ°í ¼öÁØÀÇ Á¤È®¼º°ú Taq DNA PolymeraseÀÇ ³ôÀº ÁõÆø È¿À²À» °âºñÇÑ premix mix typeÀÇ È¿¼Ò·Î, ´Ù¾çÇÑ ÁÖÇü·®¿¡ ´ëÇÑ ÀûÀÀ¼ºÀ» Çâ»ó½ÃÄÑ cDNA Ŭ·Î´× µî Á¤È®¼ºÀÌ ÇÊ¿äÇÑ Áß¿äÇÑ ¹ÝÀÀ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°ÀÇ Ç¥ÁØ ÇÁ·ÎÅäÄÝÀÎ 2-step RT-PCR¿¡¼­´Â ´ÙÀ½°ú °°Àº Ư¡À» ³ªÅ¸³½´Ù.

  • È¿À²ÀûÀ¸·Î Á¤È®¼ºÀÌ ³ôÀº RT-PCR ÁõÆø »ê¹°À» ¾òÀ» ¼ö ÀÖ´Ù.
  • Ç¥ÁØÀûÀÎ ¿ªÀü»ç ¹ÝÀÀ¿Âµµ(42¡É)¿¡¼­ °íÂ÷±¸Á¶¸¦ °¡Áø ÁÖÇü RNA¸¦ ÀÌ¿ëÇÑ ¹ÝÀÀ¿¡µµ ¶Ù¾î³­ ½ÅÀ强À» ³ªÅ¸³½´Ù.
  • ¹ÝÀÀ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Â total RNA ¾çÀÇ ¹üÀ§°¡ ¸Å¿ì ³Ð°í, »ç¿ëÇϱ⠽±´Ù.

º» Á¦Ç°ÀÇ ÇÁ·ÎÅäÄÝÀ» ÀϺΠº¯°æÇϸé, 1 step RT-PCR¿¡µµ ÀÌ¿ë°¡´ÉÇÏ´Ù.
Çü»ó
(50ȸ¿ë)*1
1. PrimeScript RTase(for 2 step) 25 ¥ìl
2. 5¡¿PrimeScript Buffer 200 ¥ìl
3. RNase Inhibitor (40 U/¥ìl) 25 ¥ìl
4. dNTP Mixture (10 mM each) 50 ¥ìl
5. Oligo dT Primer (2.5 ¥ìM) 50 ¥ìl
6. Random 6 mers (20 ¥ìM) 50 ¥ìl
7. PrimeSTAR Max Premix (2¡¿) 625 ¥ìl¡¿2
8. Control F-1 Primer*2 (20 ¥ìM) 10 ¥ìl
9. Control R-1 Primer*3 (20 ¥ìM) 10 ¥ìl
10. Positive Control RNA (2¡¿105 copies/¥ìl) 20 ¥ìl
11. RNase Free dH2O 1 ml


*1¡¡[¿ªÀü»ç ¹ÝÀÀ 20 ¥ìl ¡æ PCR ¹ÝÀÀ 50 ¥ìl] °æ¿ìÀÇ 50ȸºÐ
*2¡¡Positive Control RNA upstream sense primer
*3¡¡Positive Control RNA downstream antisense primer
º¸Á¸
- 20¡É
£¼ÁÖÀÇ£¾PrimeSTAR Max Premix(2¡¿)´Â 10ȸ Á¤µµÀÇ µ¿°á À¶ÇØ¿¡¼­´Â È°¼º ÀúÇϸ¦ º¼ ¼ö ¾ø¾úÁö¸¸, ¹Ýº¹ÀûÀÎ µ¿°á À¶ÇØ¿¡ ÀÇÇÑ È°¼º ÀúÇϸ¦ ¸·±â À§ÇØ, ÀÏ´Ü À¶ÇØ ÈÄ´Â PCR Æ©ºê¿¡ 25 ¥ìl¾¿ ºÐÁÖÇØ - 20¡ÉÀ¸·Î º¸Á¸ÇÏ´Â °ÍÀ» Ãßõ ÇÑ´Ù.
Ư¡

2 step RT-PCR(Ç¥ÁØ ÇÁ·ÎÅäÄÝ)ÀÇ °æ¿ì

RNA template

Àü¹ÝÀûÀ¸·Î Àû¿ë°¡´É

ÁõÆø »çÀÌÁî

6 kb¿¡¼­ ¾çÈ£ÇÑ ÁõÆø È®ÀÎ

¿ªÀü»çÈ¿¼Ò

PrimeScript RTase(ÃÖÀû ¹ÝÀÀ¿Âµµ:42 ¡É)

DNA Polymerase

PrimeSTAR Max DNA Polymerase(2¡¿premix type)

RNase Inhibitor

ÇÊ¿ä(Á¦Ç°³» Æ÷ÇÔ)

1st strand cDNA
ÇÕ¼º¿ë ÇÁ¶óÀ̸Ó

Random 6 mers, Oligo dT Primer, specific primer Áß ¼±Åð¡´É


1 step RT-PCR(Ç¥ÁØ ÇÁ·ÎÅäÄÝ)ÀÇ °æ¿ì

RNA template

Àü¹ÝÀûÀ¸·Î Àû¿ë°¡´É

ÁõÆø »çÀÌÁî

6 kb¿¡¼­ ÁõÆø È®ÀÎ

¿ªÀü»çÈ¿¼Ò

PrimeScript RTase(50 ¡É¿¡¼­ ¹ÝÀÀ)

DNA Polymerase

PrimeSTAR Max DNA Polymerase(2¡¿premix type)

RNase Inhibitor

ÇÊ¿ä(Á¦Ç°³» Æ÷ÇÔ)

1st strand cDNA
ÇÕ¼º¿ë ÇÁ¶óÀ̸Ó

Specific primer(PCR ¹ÝÀÀ½Ã »ç¿ëÇÏ´Â antisense primer) »ç¿ë
ÁÖ£ºRandom primer³ª Oligo dT primer »ç¿ë ºÒ°¡

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1°³ÀÇ Æ©ºê³»¿¡¼­ ¿¬¼ÓÀûÀ¸·Î RT-PCR¸¦ ½Ç½ÃÇÑ´Ù


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