¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
PCR
>
High fidelity PCR: PrimeSTAR
> HS (Premix)
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
R040A
PrimeSTAR¢ç HS (Premix)
100 ȸ
(50 ¥ìl ¹ÝÀÀ ½Ã)
160,000¿ø
200,000¿ø
09.02 ~ 10.31
,
Takara
R040B
PrimeSTAR¢ç HS (Premix)
400 ȸ (R040A x 4)
640,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ´ÙÄ«¶ó°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ High-Fidelity PCR È¿¼ÒÀÎ PrimeSTAR HS DNA Polymerase¿Í ¹ÝÀÀ¿ë buffer, dNTP Mixture¸¦ 2 ¹è ³óµµ·Î È¥ÇÕÇÑ Premix PCR È¿¼ÒÀÌ´Ù. ¹ÝÀÀ¾× Á¶Á¦°¡ °£ÆíÇÏ°í contamination À§Çèµµ ³·À¸¹Ç·Î high throughput ½ÇÇè°è¿¡ À¯¿ëÇÏ´Ù.
º» Á¦Ç°¿¡ »ç¿ëµÇ°í ÀÖ´Â PrimeSTAR HS DNA Polymerase´Â ¸Å¿ì °·ÂÇÑ 3' ¡æ 5' exonuclease È°¼ºÀ» °¡Á®, PCR ÁõÆø¿¡ ´ëÇØ °·ÂÇÑ Proofreading ±â´ÉÀ» ³ªÅ¸³»´Â ÇÑÆí, Taq DNA PolymeraseÀÇ ¸Å¿ì ¶Ù¾î³ ÁõÆø È¿À²µµ ³ªÅ¸³½´Ù.
»ó¿Â¿¡¼ÀÇ DNA Polymerase È°¼º ¹× 3' ¡æ 5' exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â monoclonal Ab¸¦ ÷°¡ÇÏ°í Àֱ⠶§¹®¿¡, PCR ¹ÝÀÀÀüÀÇ miss-primingÀ̳ª primerÀÇ digestionÀ» ¸·À» ¼ö ÀÖ´Ù. ¶ÇÇÑ º» È¿¼Ò´Â ³ôÀº priming È¿À²À» °¡Áö°í Àֱ⠶§¹®¿¡, annealing ½Ã°£À» ª°Ô ¼³Á¤ÇÒ ¼ö ÀÖ¾î Á¾·¡ÀÇ ¹ÝÀÀ ½Ã°£À» ´ÜÃàÇÒ ¼ö ÀÖ´Ù. °Ô´Ù°¡ °íµµ·Î ÁöÀûÈÇÑ PrimeSTAR Buffer¸¦ ÀÌ¿ëÇÏ´Â °ÍÀ¸·Î, Æø³ÐÀº Ÿ°Ù¿¡ ´ëÇØ High Fidelity, °í°¨µµ, °íƯÀ̼º, ³ôÀº ¼º°øÀ²·Î ÁõÆøÀÌ µÇ¾ú´Ù.
³»¿ë¹°(100ȸ ¹ÝÀÀºÐ)
PrimeSTAR HS (Premix ) : 500 ¥ìl¡¿5
Á¶¼º :
PrimeSTAR HS DNA Polymerase
1.25 U/25 ¥ìl
dNTP Mixture
2 ¡¿conc.£»°¢ 0.4 mM
PrimeSTAR Buffer
2 ¡¿conc.£»Mg
2+
= 2 mM
º¸Á¸ -20¡É
¡Ø µ¿°á À¶ÇØÀÇ ¹Ýº¹¿¡ ÀÇÇØ È°¼ºÀÌ ÇöÀúÇÏ°Ô ¶³¾îÁú ¼ö ÀÖ´Ù. À¶ÇØ ÈÄ¿¡´Â PCR¿ë tube¿¡ ºÐÁÖÇÏ¿©£20 ¡É º¸Á¸ÇØ Áֽñ⠹ٶø´Ï´Ù(50 ¥ìl ¹ÝÀÀÀÇ °æ¿ì, 25 ¥ìl µÎ°³·Î).
ÀϹÝÀûÀÎ PCR ¹ÝÀÀ Á¶¼º(Total 50 ¥ìl)
»ç¿ë·®
ÃÖÁ¾ ³óµµ
PrimeSTAR HS (Premix)
25 ¥ìl
1 ¡¿
Primer 1
10 ~15 pmol
0.2 ~0.3 ¥ìM
Primer 2
10 ~15 pmol
0.2 ~0.3 ¥ìM
Template
<200 ng
dH
2
O
up to 50 ¥ìl
PCR Á¶°Ç
(A ) 3 step PCR ÀÇ °æ¿ì
98 ¡É
10 sec.
¦¯
55 ¡É
5 sec. ¶Ç´Â 15 sec.
¦ 30 cycles
72 ¡É
1 min. /kb
¦°
(B ) 2 step PCR ÀÇ °æ¿ì
98 ¡É
10 sec.
] 30 cycles
68 ¡É
1 min. /kb
º» È¿¼ÒÀÇ °æ¿ì ÀϹÝÀûÀ¸·Î 3 step PCR ¹ÝÀÀÀ» ±ÇÀåÇÕ´Ï´Ù.
- Denature Á¶°Ç 98 ¡É
5 ~10 sec.¸¦ ±ÇÀåÇÑ´Ù. 94 ¡É´Â 10~15 sec.·Î ¼³Á¤ÇϽñ⠹ٶø´Ï´Ù.
- Annealing ¿Âµµ
¿ì¼± 55 ¡É¿¡¼ test Çغ¸½Ã±â ¹Ù¶ø´Ï´Ù.
- Annealing ½Ã°£
Tm °ª(¾Æ·¡ Âü°í)ÀÌ 55 ¡É ÀÌ»óÀÇ °æ¿ì ¡æ 5 sec.·Î ¼³Á¤
Tm °ª(¾Æ·¡ Âü°í)ÀÌ 55 ¡É ¹Ì¸¸ÀÇ °æ¿ì ¡æ 15 sec.·Î ¼³Á¤
¡Ø Tm °ª °è»ê¹æ¹ý
Tm °ª(¡É)=2 (NA£«NT)£«4 (NC£«NG)£5
PrimerÀÇ ±æÀÌ°¡ 25 mer ÀÌÇÏÀÇ °æ¿ì¿¡ Àû¿ëÇϽñ⠹ٶø´Ï´Ù. 25 mer°¡ ³Ñ´Â °æ¿ì´Â annealing ½Ã°£À» 5 sec.·Î ¼³Á¤.
<Áß¿ä> º» È¿¼Ò´Â priming È¿À²ÀÌ ¸Å¿ì ³ôÀº È¿¼ÒÀ̹ǷÎ, annealing ½Ã°£Àº5 sec. ¶Ç´Â 15 sec.·Î ¼³Á¤ÇÏ°í ¹ÝÀÀÇϽñ⠹ٶø´Ï´Ù. Annealing ½Ã°£ÀÌ ±æ¾îÁö¸é smearing Çö»óÀÌ »ý±â´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
3 step¿¡¼ smearing Çö»óÀÌ ¹ß»ýÇÏ´Â °æ¿ì, ¶Ç´Â Tm °ªÀÌ 70 ¡É ÀÌ»óÀÎ primer¸¦ »ç¿ëÇÏ´Â °æ¿ì¿¡´Â 2 stepÀÇ ¹ÝÀÀÀ» ±ÇÀåÇÕ´Ï´Ù.
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit:
Mighty TA-cloning Reagent Set for PrimeSTAR
¢ç
- 15ºÐ ¿Ï·á! ¿øÁ¶ PCR cloning kit:
In-Fusion
¢ç
Snap Assembly Master Mix
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit:
PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose:
SeaPlaque
¢ç
Agarose
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â:
Clontech PCR Thermal Cycler GP
Keyword :
High fidelity PCR: PrimeSTAR
Long fragment ÁõÆø ¹× GC-rich template ÁõÆø..
Á¤È®ÇÑ GXL DNA Polymerase
Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type
GXL Premix ŸÀÔ
ÃÖ°í ¼öÁØÀÇ Á¤È®¼ºÀº À¯Áö, °í¼Ó PCR ¼º´É ´ë..
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í ·¹º§ÀÇ Á¤È®¼º £¦ ÃÖ°í ¼ÓµµÀÇ ½ÅÀ强
ÃÖ°í Á¤È®µµ-Max DNA Polymerase
High Fidelity PCR È¿¼ÒÀÇ ±âÁØ
񃧯-HS DNA Polymerase
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
GC-rich Template¿¡ °ÇÑ High Fidelity PCR È¿..
HS with GC Buffer
Pyrobest DNA Polymerase
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus