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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Takara) > PrimeScript¢â II 1st strand cDNA Synthesis Kit
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length cDNA ÇÕ¼º kit

PrimeScript¢â II 1st strand cDNA Synthesis Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
6210A
PrimeScript¢â II 1st strand cDNA Synthesis Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
50 ȸ
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6210A_B_e.v1703Da.pdf
Takara
6210B
PrimeScript¢â II 1st strand cDNA Synthesis Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ (6210A x 4)
1,016,000¿ø 



°íÂ÷±¸Á¶ RNA¿¡ ´ëÇÑ Àû¿ë¼ºÀÌ ´õ¿í °­È­µÈ ´ÙÄ«¶óÀÇ PrimeScript™ II 1st strand cDNA Synthesis Kit!
±¸¸Å Àü¿¡ PrimeScript™ II RTase »ùÇÃÀ» ¸ÕÀú °æÇèÇغ¸¼¼¿ä. 
»ùÇýÅûÆäÀÌÁö ¹Ù·Î°¡±â!
  • ¾Ç¼¼¼­¸® ´Ü¹éÁúÀ» ÅëÇØ background ¹ÝÀÀÀ» ÇÑ°èÄ¡±îÁö ¾ïÁ¦ÇÏ°í °íÇ°Áú cDNA ÇÕ¼º
  • Works on challenging templates: GC-rich ¹× °íÂ÷±¸Á¶ÀÇ RNA¿¡¼­µµ ³ôÀº ÇÕ¼º È¿À²
  • High fidelity: ³ôÀº Á¤È®µµÀÇ cDNA ÇÕ¼º, Cloning ½ÇÇè¿¡ ÃÖÀû
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°íÂ÷±¸Á¶ÀÇ RNA³ª ±æÀÌ°¡ ±ä RNA¸¦ ÁÖÇüÀ¸·Î »ç¿ëÇÏ´Â °æ¿ì RNAÀÇ °íÂ÷±¸Á¶¿Í ¿ªÀü»ç È¿¼ÒÀÇ ºñƯÀÌÀû °áÇÕÀ¸·Î ÀÎÇØ cDNAÀÇ ÇÕ¼ºÀÌ ÀúÇصȴÙ. ¶ÇÇÑ ¿ªÀü»ç È¿¼ÒÀÇ mis-priming¿¡ ÀÇÇÑ ºñ ƯÀÌÀûÀÎ cDNA ÇÕ¼ºÀÇ °æ¿ì full-lengthÀÇ cDNA ÇÕ¼º¿¡ Å« ¾Ç¿µÇâÀ» ¹ÌÄ¡°Ô µÈ´Ù. PrimeScript™ II RTase´Â ±âÁ¸ PrimeScript™ RTase¿¡ ¾Ç¼¼¼­¸® ´Ü¹éÁúÀ» Ãß°¡ÇÏ¿© cDNA ÇÕ¼º ½Ã ¿©·¯ ÀúÇØ ¿äÀÎ (¨ç°íÂ÷±¸Á¶ÀÇ RNA, ¨èmis-priming¿¡ ÀÇÇÑ ½ÅÀå ¨é¿ªÀü»çÈ¿¼ÒÀÇ ºñƯÀÌÀû °áÇÕ)À» ±ØÇѱîÁö ¾ïÁ¦Çß´Ù. ¶ÇÇÑ PrimeScript™ II RTase´Â 42 ¡ÉÀÇ ³·Àº ÇÕ¼º¿Âµµ¿¡¼­ ¹ÝÀÀÇϱ⿡ RNAÀÇ degradationÀ» ÃÖ¼ÒÈ­ÇÒ ¼ö ÀÖ°í, background ¹ÝÀÀÀ» ÃÖ¼ÒÈ­ÇÏ¸ç °íÇ°ÁúÀÇ °íÇ°ÁúÀÇ full-lengthÀÇ cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù. ÀÌ È¿¼Ò´Â mis-priming¿¡ ÀÇÇÑ ºñƯÀÌÀû cDNA ÇÕ¼ºÀÌ ¾ïÁ¦µÇ¾î Àֱ⠶§¹®¿¡ pre-incubation ÈÄ ¾óÀ½¿¡¼­ ÃÖ´ë 30ºÐ±îÁö ¹æÄ¡ÇÏ¿©µµ ºñƯÀÌÀû ÇÕ¼º ¾øÀÌ Æí¸®ÇÏ°Ô cDNA ÇÕ¼ºÀÌ °¡´ÉÇÏ´Ù.
ÀÌ Á¦Ç°Àº total RNA ȤÀº poly A+ RNA·Î ´ÜÀÏ °¡´ÚÀÇ cDNA¸¦ ÇÕ¼ºÇϱâ À§ÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ¾î °ð¹Ù·Î ½ÇÇèÀ» ½ÃÀÛÇÒ ¼ö ÀÖ´Ù. ÇÕ¼ºµÈ cDNA´Â ÀÌÁß°¡´Ú cDNA ÇÕ¼º, hybridization, PCRµî ´Ù¾çÇÑ ½ÇÇè¿¡ »ç¿ëÇÒ ¼ö ÀÖ¾î full-length cDNA library Á¦ÀÛ µî °íÇ°ÁúÀÇ full-length cDNA°¡ ÇÊ¿äÇÑ ½ÇÇè¿¡ ÀûÀýÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50ȸ ºÐ·®)

PrimeScript II RTase (200 U/§¡)

50 §¡

5×PrimeScript II Buffer

200 §¡

RNase Inhibitor (40 U/§¡}

25 §¡

dNTP Mixture (10 mM each)

50 §¡

Oligo dT Primer (50 μM)

50 §¡

Random 6 mers (50 μM)

100 §¡

RNase free dH2O

1 §¢

º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
- PCR, cloning, sequencingµîÀÇ ½ÇÇè¿ë cDNA ÇÕ¼ºÀÇ ³¡ÆÇ¿Õ, PrimeScript™ IV 1st strand cDNA Synthesis Mix (Code 6215A)
- Real-Time PCR¿ë cDNA¸¦ gDNA ¿À¿° ¾øÀÌ ÇÕ¼º, PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Code RR047A)
- gDNA Eraser ColumnÀ¸·Î gDNA ¿À¿° ¾øÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ RNA Á¤Á¦, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
- ½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇϱâ

Keyword : cloning, qPCR, real time PCR, cDNA, Ŭ·Î´×

Reverse Transcription (Takara)
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