| 1. Reverse Transcriptase (M-MLV) (200 U/㎕) |
5 ㎕ |
| 2. RNase Inhibitor (20 U/㎕) |
5 ㎕ |
| 3. Oligo (dT)18 Anchor Primer (1 ㎍/㎕) |
10 ㎕ |
| 4. 5×1st Strand Synthesis Buffer |
40 ㎕ |
| 5. 1st Strand dNTP Mixture |
6 ㎕ |
| 6. E. coli RNase H/E. coli DNA Ligase Mixture |
10 ㎕ |
| 7. E. coli DNA Polymerase I (20 U/㎕) |
10 ㎕ |
| 8. 2nd Strand dNTP Mixture |
23 ㎕ |
| 9. 5×2nd Strand Synthesis Buffer |
150 ㎕ |
| 10. T4 DNA Polymerase (1 U/㎕) |
20 ㎕ |
| 11. 10×T4 DNA Ligase Buffer |
20 ㎕ |
| 12. T4 DNA Ligase (350 U/㎕) |
20 ㎕ |
| 13. EcoR I-Sma I Adaptor (0.4 ㎍/㎕) |
18 ㎕ |
| 14. Not I Supplement |
135 ㎕ |
| 15. Not I (50 U/㎕) |
15 ㎕ |
| 16. tRNA (10 ㎍/㎕) |
5 ㎕ |
| 17. Dr. GenTLE Precipitation Carrier |
60 ㎕ |
| 18. 3 M Sodium Acetate (pH5.2) |
1 ㎖ |
| 19. pAP3neo Predigested Vector (100 ng/㎕)*1 |
5 ㎕ |
| 20. RNase-free H2O |
640 ㎕ |
| 21. Control RNA (1 ㎍/㎕) |
5 ㎕ |
| 22. T7 promotor primer*2 (5 pmol/㎕) |
20 ㎕ |
| 23. T3 promotor primer (for pAP3neo)*6 (5 pmol/㎕) |
20 ㎕ |
| 24. Spin column |
5 ea |
