1. Reverse Transcriptase (M-MLV) (200 U/§¡) |
5 §¡ |
2. RNase Inhibitor (20 U/§¡) |
5 §¡ |
3. Oligo (dT)18 Anchor Primer (1 §¶/§¡) |
10 §¡ |
4. 5¡¿1st Strand Synthesis Buffer |
40 §¡ |
5. 1st Strand dNTP Mixture |
6 §¡ |
6. E. coli RNase H/E. coli DNA Ligase Mixture |
10 §¡ |
7. E. coli DNA Polymerase I (20 U/§¡) |
10 §¡ |
8. 2nd Strand dNTP Mixture |
23 §¡ |
9. 5¡¿2nd Strand Synthesis Buffer |
150 §¡ |
10. T4 DNA Polymerase (1 U/§¡) |
20 §¡ |
11. 10¡¿T4 DNA Ligase Buffer |
20 §¡ |
12. T4 DNA Ligase (350 U/§¡) |
20 §¡ |
13. EcoR I-Sma I Adaptor (0.4 §¶/§¡) |
18 §¡ |
14. Not I Supplement |
135 §¡ |
15. Not I (50 U/§¡) |
15 §¡ |
16. tRNA (10 §¶/§¡) |
5 §¡ |
17. Dr. GenTLE Precipitation Carrier |
60 §¡ |
18. 3 M Sodium Acetate (pH5.2) |
1 §¢ |
19. pAP3neo Predigested Vector (100 ng/§¡)*1 |
5 §¡ |
20. RNase-free H2O |
640 §¡ |
21. Control RNA (1 §¶/§¡) |
5 §¡ |
22. T7 promotor primer*2 (5 pmol/§¡) |
20 §¡ |
23. T3 promotor primer (for pAP3neo)*6 (5 pmol/§¡) |
20 §¡ |
24. Spin column |
5 ea |