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cDNA Library ±¸Ãà All-in-One Kit

cDNA Library Construction Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Takara
6136
cDNA Library Construction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ȸ
796,000¿ø  6136_Korea.pdf

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1. Reverse Transcriptase (M-MLV) (200 U/§¡) 5 §¡
2. RNase Inhibitor (20 U/§¡) 5 §¡
3. Oligo (dT)18 Anchor Primer (1 §¶/§¡) 10 §¡
4. 5¡¿1st Strand Synthesis Buffer 40 §¡
5. 1st Strand dNTP Mixture 6 §¡
6. E. coli RNase H/E. coli DNA Ligase Mixture 10 §¡
7. E. coli DNA Polymerase I (20 U/§¡) 10 §¡
8. 2nd Strand dNTP Mixture 23 §¡
9. 5¡¿2nd Strand Synthesis Buffer 150 §¡
10. T4 DNA Polymerase (1 U/§¡) 20 §¡
11. 10¡¿T4 DNA Ligase Buffer 20 §¡
12. T4 DNA Ligase (350 U/§¡) 20 §¡
13. EcoR I-Sma I Adaptor (0.4 §¶/§¡) 18 §¡
14. Not I Supplement 135 §¡
15. Not I (50 U/§¡) 15 §¡
16. tRNA (10 §¶/§¡) 5 §¡
17. Dr. GenTLE Precipitation Carrier 60 §¡
18. 3 M Sodium Acetate (pH5.2) 1 §¢
19. pAP3neo Predigested Vector (100 ng/§¡)*1 5 §¡
20. RNase-free H2O 640 §¡
21. Control RNA (1 §¶/§¡) 5 §¡
22. T7 promotor primer*2 (5 pmol/§¡) 20 §¡
23. T3 promotor primer (for pAP3neo)*6 (5 pmol/§¡) 20 §¡
24. Spin column 5 ea
*1 EcoR I, Not IÀ¸·Î Àý´ÜµÈ »óÅÂÀÌ´Ù.
*2 Insert üũ ¹× Sequencing¿¡ »ç¿ë°¡´É
º¸Á¸
Dr. GenTLE Precipitation Carrier, 3 M Sodium Acetate (pH5. 2), ½ºÇÉ Ä÷³£º 4¡É
±× ¿Ü Kit³» ½Ã¾à·ù£º - 20¡É
Á¦Ç°¼³¸í
ÁøÇÙ»ý¹°ÀÇ ¿©·¯ Á¶Á÷À̳ª ¼¼Æ÷¿¡¼­ Á¶Á¦ÇÑ poly(A)+ RNA·ÎºÎÅÍ cDNA¸¦ ÇÕ¼ºÇÏ¿© Ŭ·Î´×ÇÏ´Â ±â¼úÀº ºÐÀÚ»ý¹°ÇÐÀû ¿¬±¸¿¡ À־ Áß¿äÇÑ ¹æ¹ýÀÇ Çϳª·Î À¯ÀüÀÚÀÇ ±¸Á¶Çؼ®À̳ª ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö Á¶ÀÛ¿¡ Ȱ¹ßÇÏ°Ô Àû¿ëµÇ°í ÀÖ´Ù.
ÀϹÝÀûÀ¸·Î cDNA ÇÕ¼º°ú library´Â ¸ñÀû poly(A)+ RNA¿¡ »óº¸ÀûÀÎ double strand cDNA¸¦ ÇÕ¼ºÇØ ¹ÚÅ׸®¾Æ³ª ¹ÙÀÌ·¯½º À¯·¡ÀÇ vector¿¡ Ŭ·Î´×ÇÏ¿© ÀÌ cDNA ÀçÁ¶ÇÕü¸¦ ¹ÚÅ׸®¾Æ ȤÀº ÁøÇÙ¼¼Æ÷¿¡ µµÀÔÇÏ¿© º¹Á¦ÇÑ ÈÄ cDNA¸¦ ÁõÆø½ÃÄÑ, cDNAÀÇ ÇØ¼®À» ½Ç½ÃÇϰųª in vitro transcription ¶Ç´Â in vitro translation µî¿¡ ÀÌ¿ëµÈ´Ù.
º» Á¦Ç°Àº ÁÖ·Î µ¿½Ä¹° À¯·¡ÀÇ poly(A)+ RNA·ÎºÎÅÍ double strand cDNA¸¦ ÇÕ¼ºÇÑ ÈÄ, ÷ºÎµÈ pAP3neo µîÀÇ plasmid vector¿¡ ÀçÁ¶ÇÕÇÏ¿© cDNA library¸¦ ±¸ÃàÇϱâ À§ÇÑ KitÀÌ´Ù. cDNA libraryÀÇ ±¸Ãà¿¡´Â Gubler-Hoffman ¹æ¹ý¿¡ ±Ù°ÅÇÑ linker-primer ¹ýÀ» »ç¿ëÇϰí ÀÖ¾î, À¯ÀüÀÚÀÇ ¹æÇ⼺À» À¯ÁöÇÏ´Âdirectional cloningÀÌ °¡´ÉÇÏ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
º» Á¦Ç°¿¡´Â ÇüÁúÀüȯ¿¡ ÇÊ¿äÇÑ ´ëÀå±ÕÀº Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù. ¸Þƿȭ DNA¿¡ ÀÇÇÑ ÇüÁúÀüȯ(electroporation¹ý)ÀÌ °¡´ÉÇÑ ´ëÀå±Õ, E. coli HST02 Electro-Cells (Code 9026), ClontechÀÇ Supercharge EZ10 Electrocompetent Cells(Code 636756) µîÀ» º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù.

Keyword :

Reverse Transcription (Takara)
>
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
>
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
>
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
>
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
>
RT-PCR Çʼöǰ
Recombinant RNase Inhibitor
>
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
>
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer