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ÀüÁ¦Ç°º¸±â
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cDNA ÇÕ¼º
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Reverse Transcription (Takara)
> PrimeScript¢â Reverse Transcriptase
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ standard
PrimeScript¢â Reverse Transcriptase
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Takara
2680A
PrimeScript¢â Reverse Transcriptase
10,000 U
167,200¿ø
209,000¿ø
03.02 ~ 04.30
Takara
2680B
PrimeScript¢â Reverse Transcriptase
40,000 U
752,400¿ø
Product index
Reverse Transcription (Tak..
Reverse Transcriptase ..
High Fidelity, Full Le..
PrimeScript¢â IV 1st s..
PrimeScript¢â II 1st s..
PrimeScript¢â Reverse ..
PrimeScript¢â 1st stra..
Reverse Transcriptase ..
Recombinant RNase Inhi..
Reverse Transcriptase ..
Ribonuclease Inhibitor
cDNA Library Construct..
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (..
Double strand cDNA ÇÕ..
Oligo(dT) 15 primer
Reverse Transcription (Clo..
[°³¿ä] So Easy Product
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
MMLV Reverse Transcrip..
SMARTScribe Reverse Tr..
Advantage RT-for-PCR K..
SMART MMLV Reverse Tra..
TITANIUM One-Step RT-P..
SMART¢ç Full length cDNA ..
[°³¿ä] SMARTer¢ç cDNA ..
RACE 5/3 Kit (Unknown ..
Full length cDNA Synth..
Marathon cDNA Amplific..
cDNA Library Construct..
In-Fusion¢ç SMARTer cD..
cDNA Library (Human, Mouse..
Human Brain cDNA Libra..
Human cDNA Library
Mouse cDNA Library
Rat cDNA Library
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ ..
QUICK-Clone cDNA (Huma..
QUICK-Clone cDNA (Mous..
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
Total RNA£¦Poly A RNA
Human Tumor, Human Cel..
Premium Poly A+ RNA (H..
Premium Poly A+ RNA (H..
Premium Total RNA (Hum..
Premium Total RNA (Mou..
Premium Total RNA (Rat..
Premium Total RNA (Bov..
qPCR Human Reference c..
Total RNA Master Panel
Universal Reference To..
±× ¿Ü °ü·ÃÁ¦Ç° (RNA)
RNase-free Water
RNase-OFF¢â
³óµµ 200 U/§¡
Çü»ó
20 mM
Tris-HCl (pH7.8)
100 mM
NaCl
1 mM
EDTA
1 mM
DTT
50%
Glycerol(v/v)
º¸Á¸ -20¡É
5¡¿ PrimeScript Buffer (cDNA ÇÕ¼º¿ë)
250 mM
Tris-HCl (pH8.3)
375 mM
KCl
15 mM
MgCl
2
Á¦Ç°¼³¸í
º» Á¦Ç°Àº M-MLV(Moloney Murine Leukemia Virus) À¯·¡ÀÇ Reverse Transcriptase¸¦ ±â¹ÝÀ¸·Î ÇÏ¿© ´ÙÄ«¶ó°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ½Å±Ô ¿ªÀü»ç È¿¼ÒÀÌ´Ù. º» Á¦Ç°Àº ½ÅÀ强ÀÌ ¸Å¿ì ¶Ù¾î³ª ±ä cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù. ¶Ç, GC rich µî°ú °°ÀÌ cDNA ÇÕ¼ºÀÌ ¾î·Á¿î RNA¿¡
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ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù. º» È¿¼Ò´Â ±ä cDNAÀÇ Á¶Á¦³ª full length cDNAÀÇ ºñÀ²ÀÌ ³ôÀº library Á¦ÀÛ¿¡ ÃÖÀûÀÌ´Ù.
PrimeScrip RTase ÀÌ¿ë 42¡É ¹ÝÀÀ¿Âµµ·Î, ¹é±×¶ó¿îµå°¡ ³·Àº °íǰÁúÀÇ cDNA ÇÕ¼º °¡´É
RNA Ladder (1~12kb)¸¦ ÁÖÇüÀ¸·Î, PrimeScript ¹× Ÿ»ç RTase¸¦ ÀÌ¿ëÇÏ¿© ¿ªÀü»ç ¹ÝÀÀ.
Ȱ¼ºÀÇ Á¤ÀÇ
Poly(rA)- oligo(dT)
12-18
¸¦ ÁÖÇü/ÇÁ¶óÀ̸ӷΠÇÏ¿© 37¡É, 10ºÐ°£ 1 nmolÀÇ [
3
H]dTTP¸¦ ÇÕ¼º±âÁú·Î »ç¿ëÇÏ´Â °ÍÀ» È¿¼Ò Ȱ¼º 1UÀ¸·Î ÇÑ´Ù..
Ȱ¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM
Tris-HCl (pH8.3)
75 mM
KCl
8 mM
MgCl
2
10 mM
DTT
20 ug/ml
(rA)n - (dT)
12-18
0.5 mM
[
3
H]dTTP
0.1%
NP-40
1st-strand cDNA ÇÕ¼º½ÃÇè
Ç¥ÁØ ½ÇÇè¹ý(ÇÁ·ÎÅäÄÝ)¿¡ µû¶ó ·§Æ® ³ú À¯·¡ÀÇ Total RNA 100 ngÀ» ÁÖÇüÀ¸·Î ÇÏ¿© Oligo dT ÇÁ¶óÀÌ¸Ó 50 pmol ¹× º» È¿¼Ò 200 unitsÀ»
ÀÌ¿ëÇÏ¿© 42¡É¿¡¼ 60ºÐ°£ 1st-strand cDNA ÇÕ¼º ¹ÝÀÀÀ» ÇÑ´Ù. ÀÌ cDNA ¹ÝÀÀ¾×À» ÁÖÇüÀ¸·Î PCR ÇÏ¿© ¾Æ°¡·ÎÁî °Ö¿¡ Àü±â ¿µµ¿ÇÑ
°á°ú 12 kbÀÇ size À§Ä¡¿¡¼ ¸íÈ®ÇÑ ¹êµå¸¦ È®ÀÎÇÏ¿´´Ù.
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1. 200 UÀÇ º» È¿¼Ò¿Í 1§¶ÀÇ ¥ëDNA-
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d III ºÐÇØ¹°À» 37¡É, 1½Ã°£ ¹ÝÀÀ½ÃÄѵµ DNA Àü±â ¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ÀϾÁö ¾Ê´Â´Ù.
2. 200 UÀÇ º» È¿¼Ò¿Í 1§¶ÀÇ supercoiled pBR322 DNA¸¦ 37¡É, 1½Ã°£ ¹ÝÀÀ½ÃÄѵµ DNA Àü±â ¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ÀϾÁö ¾Ê´Â´Ù.
3. 200 UÀÇ º» È¿¼Ò¿Í 1§¶ÀÇ 23S ¹× 16S rRNA È¥ÇÕ¹°À» 37¡É, 1½Ã°£ ¹ÝÀÀ½ÃÄѵµ RNA Àü±â ¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ÀϾÁö ¾Ê´Â´Ù.
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- 1st-strand cDNA ÇÕ¼º
- cDNA probe Á¶Á¦
- RT- PCR
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Keyword : 2640A
Reverse Transcription (Takara)
Reverse Transcriptase ¼±Åð¡À̵å
High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
Reverse Transcriptase XL (AMV)
RT-PCR Çʼöǰ
Recombinant RNase Inhibitor
RNA PCR KitÀÇ Component
Reverse Transcriptase XL (AMV) for RT-PCR Kit
Ribonuclease Inhibitor
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (Random Primer)
Double strand cDNA ÇÕ¼º
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer