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cDNA ÇÕ¼º
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Premade cDNA
> RACE ¿ë Marathon-Ready cDNA (Rat)
RACE ¿ë Marathon-Ready cDNA (Rat)
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Clontech
639412
Rat Brain Marathon-Ready cDNA
30 ȸ
1,758,000¿ø
,
Clontech
639417
Rat Testis Marathon-Ready cDNA
30 ȸ
1,758,000¿ø
,
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Marathon¢ç-Ready cDNA for RACE
RACE PCRÀ» À§ÇØ adaptor°¡ ¿¬°áµÇ¾î ÀÖ´Â cDNA
Human, mouse, ratÀÇ ¸Å¿ì ´Ù¾çÇÑ Á¶Á÷°ú ¼¼Æ÷·ÎºÎÅÍ ÇÕ¼ºµÈ cDNA
Marathon-Ready cDNA´Â °íÇ°ÁúÀÇ ÇÁ¸®¹Ì¾ö poly A+ RNA·ÎºÎÅÍ ÇÕ¼ºµÈ cDNA·Î °ð¹Ù·Î 5¡¢- RACE PCRÀ̳ª 3¡¢-RACE PCRÀ» ¼öÇàÇÒ ¼ö ÀÖµµ·Ï Marathon Adaptor¸¦ ºÙ¿© ³õÀº cDNAÀÌ´Ù (1). °¢°¢ÀÇ Marathon Ready cDNA´Â full-length geneÀÌ À¯ÀüÀÚ Æ¯ÀÌÀû primer¿¡ ÀÇÇØ ÁõÆøµÉ ¼ö ÀÖµµ·Ï Á¶Á÷ ƯÀÌÀû(tissue-specific)ÀÎ double-strand cDNA ÀÇ "pool"·Î ÀÌ·ç¾îÁø Á¦Ç°ÀÌ´Ù. ¶ÇÇÑ, º» Á¦Ç°Àº Á¶Á÷ ƯÀÌÀûÀÎ À¯ÀüÀÚ ¹ßÇö ¿¬±¸³ª mRNAÀÇ ´ÙÇü¼º ¿¬±¸, ±×¸®°í º¹¼ö À¯ÀüÀÚ ±º¿¡ ¼ÓÇÑ Æ¯Á¤ mRNA ¿¬±¸¿¡ »ç¿ëÇÒ¼ö ÀÖ´Ù.
Marathon-Ready cDNA´Â ÇÁ¸®¹Ì¾ö RNA·ÎºÎÅÍ 3¡¢-ÀÌÁú¼º (heterogeneity)À» Á¦°ÅÇÏ°í full-length cDNA¸¦ ÇÕ¼ºÇϱâ À§ÇØ ÃÖÀûÈµÈ ½ÇÇè¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© ÇÕ¼ºµÇ¾ú´Ù (2, 3). ÇÕ¼º ÈÄ ¾ç ¸»´ÜÀÌ ÆòȰȵǸç Marathon Adaptor´Â double-strand cDNAÀÇ ¾çÂÊ ³¡¿¡ ºÙ°Ô µÈ´Ù.
°¢°¢ÀÇ Marathon-Ready cDNA´Â 30ȸ »ç¿ëºÐ·® (³óµµ~ 0.1 ng/¥ìl) À¸·Î adaptor primer, nested adaptor primer, 5¡¢°ú 3¡¢G3PDH control primer°¡ Æ÷ÇԵǾî ÀÖ´Ù. Marathon-Ready cDNA User Manual (PT1156-1)Àº www.clontech.com/manuals¿¡¼ ´Ù¿î·Îµå ¹ÞÀ» ¼ö ÀÖ´Ù. Marathon-Ready cDNA¸¦ ÀÌ¿ëÇÑ 5¡¢- RACE¿Í 3¡¢-RACE ½ÇÇè¿¡´Â °¢ÀÚÀÇ À¯ÀüÀÚ Æ¯ÀÌÀû primer°¡ ÇÊ¿äÇÏ°í, PCR ¹ÝÀÀÀ» À§ÇÑ È¿¼Ò¸¦ º°µµ·Î ±¸¸ÅÇÏ¿©¾ß ÇÑ´Ù
(Advantage 2 Polymerase Mix (Cat. No. 639201 & 639202) ̵̧).
Figure 1. Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA.
RACE PCR was performed. The template for Lanes 1- 3 was Marathon-Ready Human Placenta cDNA (Cat. No.639311); the template for Lanes 4 & 5 was Marathon-Ready Human Skeletal Muscle cDNA (Cat. No. 639313). Lane 1: 5¡¯-RACE product for TFR cDNA. Lane 2: 3¡¯-RACE product for TFR cDNA. Lane 3: full-length TFR cDNA (5.1 kb) generated by end-to-end amplification using 5¡¯ and 3¡¯ TFR primers. Lane 4: nearly full-length IGFR2 cDNA (8.8 kb) amplified with 5¡¯ IGFR2 and 3¡¯ IGFR2 primers. Lane 5: full-length IGFR1 cDNA (5.0 kb) amplified with primers 5¡¯-IGFR and 3¡¯-IGFR. Lane M: 1-kb DNA size ladder.
Figure 2. Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs.
RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. No. 639304), Mouse Heart (Cat. No. 639404), and Rat Heart (Cat. No.639416) cDNAs. Lane M: 1-kb DNA size ladder.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Chenchik, A. et al. (1996) BioTechniques 21:526- 532.
2. Chenchik, A. et al. (January 1995) Clontechniques X(1):5- 8. 3.
3. Borson, N. D. et al. (1992) PCR Methods Appl. 2:144- 148.
Keyword :
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ °íÇ°Áú 1st strand cDNA Set MTC ´ÙÁ¶Á÷ cDNA Panel
QUICK-Clone cDNA (Human)
QUICK-Clone cDNA (Mouse)
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready cDNA (Human)
RACE ¿ë Marathon-Ready cDNA (Mouse)
RACE ¿ë Marathon-Ready cDNA (Rat)