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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Premade cDNA > QUICK-Clone cDNA (Human)

QUICK-Clone cDNA (Human)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
637239
Human Bone Marrow QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637243
Human Brain, thalamus QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,529,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637242
Human Brain, whole QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,529,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637220
Human Fat Cell QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637213
Human Heart QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637203
Human HeLa QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637240
Human Leukocyte QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637206
Human Lung QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637231
Human Mammary Gland QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637232
Human Pituitary Gland QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637208
Human Placenta QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637215
Human Prostate QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637216
Human Retina QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,529,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637235
Human Small Intestine QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637222
Human Spinal Cord QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,529,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637217
Human Spleen QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637218
Human Stomach QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637209
Human Testis QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637210
Human Thymus QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637236
Human Thyroid Gland QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637260
Human Universal QUICK-Clone cDNA II
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
2,952,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637237
Human Uterus QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,529,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982

QUICK-Clone cDNA
  • Library screening ¾øÀÌ PCR·Î Á÷Á¢ ¿øÇÏ´Â À¯ÀüÀÚ¸¦ cloning °¡´É
  • Human, mouse, ratÀÇ ´Ù¾çÇÑ Á¶Á÷°ú ¼¼Æ÷·ÎºÎÅÍ ÇÕ¼ºµÈ cDNA
  • ÀÌÀü¿¡ ºÐ¸®ÇÑ cDNA, ±¸Á¶ÀûÀ¸·Î °ü·ÃµÈ cDNA ¹× Á¾ °£ÀÇ cDNA¸¦ ÁõÆøÇϴµ¥ ÀûÇÕ
QUICK-Clone cDNA´Â À¯ÀüÀÚ Æ¯ÀÌÀûÀÎ primer·Î ¿øÇÏ´Â À¯ÀüÀÚ¸¦ ÁõÆøÇÒ ¼ö ÀÖµµ·Ï Á¦ÀÛµÈ double-strand cDNAÀÌ´Ù.
QUICK-Clone cDNA´Â ÀÌÀü¿¡ ºÐ¸®ÇÑ cDNA³ª ±¸Á¶ÀûÀ¸·Î °ü·ÃµÈ cDNA, cross-species cDNA¸¦ ÁõÆøÇϴµ¥ ÀûÇÕÇÏ´Ù. ¶ÇÇÑ, ¸ñÀû cDNAÀÇ ÁõÆøÀÇ À§ÇÏ¿© library¸¦ Á¦ÀÛÇÏ°í screeningÇÏ´Â ÀüÅëÀûÀÎ ¹æ¹ýÀ» »ç¿ëÇÏÁö ¾Ê¾Æµµ µÈ´Ù. ¶ÇÇÑ, QUICKClone cDNA´Â À¯ÀüÀÚ Æ¯ÀÌÀûÀÎ hybridization¿ë probe, degenerated primer¸¦ Á¦ÀÛÇÒ ¶§µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù (1~5).
°íÇ°ÁúÀÇ cDNA template´Â PCR ÁõÆø¿¡ ÁÁÀº °á°ú¸¦ °¡Á®¿Â´Ù. °íÇ°ÁúÀÇ ÇÁ¸®¹Ì¾ö poly A+ RNA·ÎºÎÅÍ oligo(dT) primer·Î ÇÕ¼ºµÈ QUICK-Clone cDNA´Â Á¤Á¦ °úÁ¤À» ÅëÇÏ¿© ÀÜ¿© RNA¿Í 400 bpÀÌÇÏÀÇ cDNA¸¦ ¸ðµÎ Á¦°ÅÇÏ¿© PCR¿¡ ÃÖÀûÀÎ °í¼øµµÀÇ double-strand cDNAÀÌ´Ù.
QUICK-Clone cDNA´Â °¢°¢ 10 ngÀÇ cDNA°¡ Æ÷ÇÔµÈ µÎ °³ÀÇ tube·Î Á¦°øµÇ¸ç, ÀÌ´Â 50 ¥ìl PCR ¹ÝÀÀ ½Ã 20ȸ¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Â ºÐ·®ÀÌ´Ù.
QUICK-Clone cDNA User Manual (PT1150-1)Àº www.clontech.com/manuals¿¡¼­ ´Ù¿î·Îµå ¹ÞÀ» ¼ö ÀÖ°í, PCRÀ» À§ÇÏ¿© TITANIUM Taq DNA Polymerase (Cat. Code 639208 & 639209) ¶Ç´Â ±ä ¼­¿­À» PCRÇÒ ¼ö ÀÖ´Â Advantage 2 Polymerase Mix (Cat. No. 639201 & 639202)¸¦ ÃßõÇÑ´Ù.
Full-length Human cDNAÀÇ È®º¸
QUICK-Clone II Human Universal cDNA (Cat. No. 639260)Àº ÀϹÝÀûÀÎ human Á¶Á÷¿¡¼­ ÇÕ¼ºµÈ 30Á¾ ÀÌ»óÀÇ QUICK-Clone cDNA°¡ ¼¯¿©ÀÖ´Â Á¦Ç°ÀÌ´Ù (Á¤È®ÇÑ ¼ýÀÚ´Â Á¶Á÷¿¡ µû¶ó ´Ù¸§). ƯÈ÷ ´ëºÎºÐÀÇ human À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏ´Â full-length cDNA ÁõÆø¿¡ ÀûÇÕÇÏ´Ù.

ÁÖ : »ç¿ëµÈ libraryÀÇ source´Â lotº°·Î ´Þ¶óÁú ¼ö ÀÖ´Ù. °¢°¢ÀÇ library source¿¡ ´ëÇÑ Á¤º¸´Â Certificate of Analysis¸¦ Âü°íÇϱ⠹ٶõ´Ù.

* QUICK-Clone XG Tumor cDNA´Â nude mouse¿¡¼­ Áõ½Ä½ÃŲ ÀÌÁ¾ÀÌ½Ä Àΰ£ Á¾¾ç (xenografted human tumors)¿¡¼­ ÇÕ¼ºµÇ¾ú´Ù. 6:149-158.

Figure 1. PCR amplification of the full coding region of the humanTFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. No.637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: ¥ë/Hind III DNA size marker. Lane M2: ¥ÕX174/Hae III DNA size marker.

Table I: Tissues Represented in QUICK-Clone II Human Universal cDNA
Adrenal Gland Fetal Lung Retina
Aorta Heart Salivary Gland
Bone Marrow Kidney Skeletal Muscle
Brain Leukocyte Small Intestine
Brain, cerebellum Liver Spinal Cord
Brain, cerebral cortex Lung Spleen
Brain, hippocampus Lymph Node Stomach
Brain, thalamus Mammary Gland Testis
Fat Cell Ovary Thymus
Fetal Brain Pancreas Thyroid Gland
Fetal Heart Pituitary Gland Uterus
Fetal Kidney Placenta
Fetal Liver Prostate
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Parmentier, M. et al. (1989) Science 246:1620-1622.
2. Wilks, A. E. et al. (1989) Gene 85:67-74.
3. Vallins, W. J. et al. (1990) FEBS Letters 270:57-61.
4. Lee, C. C. et al. (1998) Science 239:1288-1291.
5. Schuchman, E. H. et al. (1990) Genomics
6:149-158.

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