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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Takara) > ¿ªÀü»ç ¹ÝÀÀ¿ë Primer (Random Primer)

¿ªÀü»ç ¹ÝÀÀ¿ë Primer (Random Primer)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3801
Random Primer (hexadeoxyribonucleotide mixture; pd (N)6)
°ü·ÃÇмú ±¸¸ÅÇϱâ
80 nmol
155,000¿ø  3801_DS.v2109Da.pdf
Takara
3802
Random Primer (nonadeoxyribonucleotide mixture; pd (N)9)
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 nmol
155,000¿ø  3802_DS.v2109Da.pdf



Á¦Ç°¼³¸í
Random ¼­¿­À» °¡Áø °¢°¢ 6 mer ¶Ç´Â 9 merÀÇ deoxyribonucleotide mixture·Î 5¡¯-¸»´ÜÀÌ ÀλêÈ­ µÇ¾î ÀÖ´Ù.
µÎ Á¦Ç° ¸ðµÎ ¾à 150 §¶, 5 OD°¡ Á¦°øµÈ´Ù.
º¸Á¸ -20¡É
Primer ¼­¿­
Random Primer (hexadeoxyribonucleotide mixture)£ºpd(N)6
Random Primer (nonadeoxyribonucleotide mixture)£ºpd(N)9
Çü»ó µ¿°á °ÇÁ¶Ç°
¿ëµµ
- RNA¿¡¼­ cDNA ÇÕ¼º ½Ã ¿ªÀü»ç ¹ÝÀÀ¿ë primer·Î »ç¿ë.
- RT-PCR¿¡¼­ÀÇ 1st strand ÇÕ¼º ½Ã primer·Î »ç¿ë.
- hybridization ½ÇÇèÀ» À§ÇÑ labelµÈ probe Á¦ÀÛ (120 pmol primer/§¶ DNA¸¦ ÃßõÇÑ´Ù.).
»ç¿ë»óÀÇ ÁÖÀÇ
- Random 9 mer¸¦ RNase Free dH2O 1 §¢¿¡ (ÃÖÁ¾³óµµ 50 ¥ìM) ¿ëÇØÇÏ¿© ¾Æ·¡ÀÇ Á¦Ç°¿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

- Random 6 mer¸¦ RNase Free dH2O 4 §¢¿¡ (ÃÖÁ¾³óµµ 20 ¥ìM) ¿ëÇØÇÏ¿© ¾Æ·¡ÀÇ Á¦Ç°¿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ¼º´ÉÀÌ °³·®µÈ ÃÖ½ÅÀÇ ¿ªÀü»ç¿ë All-in-One, premix
: PrimeScript¢â IV 1st strand cDNA Synthesis Mix (Code 6215A)
- cDNA ÇÕ¼º ½Ã ÀúÇØ¿äÀÎÀ» ±Øº¹ÇÑ All-in-One kit
: PrimeScript¢â II 1st strand cDNA Synthesis Kit (Code 6210A)
- Full length cDNA¸¦ Á¤È®ÇÏ°Ô ÇÕ¼ºÇÏ´Â ¿ªÀü»çÈ¿¼Ò
: PrimeScript¢â Reverse Transcriptase (Code 2680)
- gDNA Á¦°Å spin columnÀÌ Æ÷ÇÔµÈ RNA Á¤Á¦ spin column
: TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)

Keyword :

Reverse Transcription (Takara)
>
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
>
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
>
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
>
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
>
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
>
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
>
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer