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PCR
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RT-PCR °ü·Ã Á¦Ç°
> TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
Long strand cDNA ÁõÆø¿¡
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
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Takara
RR012A
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
100 ȸ
600,800¿ø
751,000¿ø
09.02 ~ 10.31
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Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
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º» Á¦Ç°Àº RNA¸¦ cDNA·Î º¯È¯ÇÑ ÈÄ¿¡ PCRÀ» ½Ç½ÃÇϱâ À§ÇÑ 2 Step RT-PCR Kit·Î AMV (Avian Myeloblastosis Virus) À¯·¡ÀÇ ¿ªÀü»çÈ¿¼Ò¿¡ ÀÇÇØ RNA·Î ºÎÅÍ cDNA ÇÕ¼º ±×¸®°í, TaKaRa LA Taq¿¡ ÀÇÇÑ cDNAÀÇ ÁõÆøÀ» ÇÑ °³ÀÇ tube ³»¿¡¼½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
Oligo-dT Adaptor Primer´Â poly(A)+ RNAÀÇ 3¡¢¸»´ÜÀ¸·ÎºÎÅÍ ¾ÆÁÖ ³ôÀº È¿À²·Î cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾î ÀÖ´Ù. µû¶ó¼ 3¡¢- RACE¹ý¿¡ ÀÇÇÑ ¹ÌÁöÀÇ 3¡¯¸»´ÜÀÇ ÁõÆø¿¡ ¿ëÀÌÇÏ´Ù.
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RNA template
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12kb¶óÁö ÁõÆø°¡´É
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AMV Reverse Transcriptase XL (42~60¡É¿¡¼ ¿ªÀü»ç°¡´É)
DNA Polymerase
TaKaRa LA Taq
RNase Inhibitor
Kit¿¡ Æ÷ÇÔ
1st strand cDNAÇÕ¼º
Random 9 mers,
ÇÕ¼º Primer
Oligo dT- Adaptor Primer
ƯÀÌÀû ÇÏ·ù primer »ç¿ë°¡´É
3¡¯- RACE¹ý
RT ¹ÝÀÀ ½Ã Oligo dT-Adaptor Primer, PCR ¹ÝÀÀ ½Ã M13
M4 Primer¸¦ »ç¿ëÇϹǷνá 3¡¯- RACE¹ýÀÌ °¡´É
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1°³ÀÇ tube ³»¿¡¼ ¹ÝÀÀ°¡´É, ¿Ã³¸®ÇÏ¿© ¿ªÀü»ç È¿¼Ò È°¼ºÀ» ½ÇÈ°½ÃŲ ÈÄ PCRÇÑ´Ù.
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*1
AMV Reverse Transcriptase XL (5 U/§¡)
50 §¡
RNase Inhibitor (40 U/§¡£©
25 §¡
Random 9 mers (50 pmol/§¡)
50 §¡
Oligo dT-Adaptor Primer (2.5 pmol/§¡)
50 §¡
RNase Free dH
2
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1 §¢
TaKaRa LA Taq (5 U/§¡)§¡)
25 §¡
M13 Primer M4 (20¥ìM)
50 §¡
10¡¿ RNA PCR Buffer
*2
120 §¡
10¡¿ LA PCR Buffer II (Mg2£« free)
5000 §¡
dNTP Mixture (°¢ 10 mM)
150§¡
MgCl
2
(25 mM)
1§¡
Control F-1 Primer (20 pmol/§¡)
(Positive Control RNA downstream primer)
25§¡
Control R-1 Primer (20 pmol/§¡)
(Positive Control RNA upstream primer)
25§¡
Positive Control RNA (2¡¿10
5
copies/§¡)
(pSPTet3 plasmidÀÇ Àü»ç poly(A)£« RNA)
25§¡
*1
[10 §¡ ¿ªÀü»ç¹ÝÀÀ ¡æ 50 §¡ PCR¹ÝÀÀ]ÀÇ 100ȸºÐ
*2
100mM Tris-HCI (pH8.3), 500mM KCI.
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
Reverse Transcriptase XL (AMV) for RT-PCR
(TaKaRa Code 2630A)
Ribonuclease Inhibitor
(TaKaRa Code 2311A)
Ribonuclease Inhibitor
(TaKaRa Code 2310A)
TaKaRa LA Taq
(TaKaRa Code RR002A)
Keyword :
RT-PCR °ü·Ã Á¦Ç°
°í¼Ó¹ÝÀÀ, ³ôÀº Á¤È®µµ ½ÇÇö - 1step RT-PCR ki..
PrimeScript¢â II High Fidelity One Step RT-PCR Kit
RT-PCR kit, ²Ä²ÄÈ÷ µûÁ®º¸°í ¼±ÅÃÇϱâ
¿ªÀü»ç ¹ÝÀÀÀÇ ÃÖÀû ¿Âµµ°¡ ³ôÀº RT-PCR ¿ë Kit
BcaBEST¢ç RNA PCR Kit Ver. 1.1
PrimeScript RTase¿Í PrimeSTAR Max¸¦ Á¶ÇÕÇÏ¿©..
PrimeScript¢â High Fidelity RT-PCR Kit
Premix·Î Æí¸®ÇÑ 1 step RT-PCR kit
PrimeScript¢â One Step RT-PCR Kit Ver.2
PrimeScript RTase £¦ TaKaRa Ex Taq HS·Î ÃÖÀû..
PrimeScript¢â RT-PCR Kit
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
SMARTer¢ç RACE 5'/3' Kit
PCR±îÁö 1 tube·Î °¡´ÉÇÑ 1 step RT-PCR ¿ë Kit
TaKaRa One Step RNA PCR Kit (AMV)
Long strand cDNA ÁõÆø¿¡
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
TaKaRa Ex Taq HS°ú AMV RTase¸¦ ÀÌ¿ëÇÑ 2 step..
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0