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Dye°¡ ÷°¡µÈ °í¼º´É PCR Premix ½Ã¾à

Colony PCR¿ë premix

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
RR310A
EmeraldAmp¢ç GT PCR Master Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
160 ȸ
(50 ¥ìl ¹ÝÀÀ°è)
70,400¿ø 
88,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR310A_DS.v1902Da.pdf
Takara
RR310B
EmeraldAmp¢ç GT PCR Master Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (RR310A x 5)
355,000¿ø 

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EmeraldAmp GT PCR Master Mix´Â routine PCR ½ÇÇè¿¡ ÃÖÀûÈ­µÈ PCR Master MixÀ̸ç, GC ¶Ç´Â AT rich ¿µ¿ª¿¡¼­µµ ³ôÀº ÁõÆø È¿À²À» ¹ßÈÖÇÑ´Ù. PCR¿¡ ÇÊ¿äÇÑ ±¸¼º¿ä¼Ò (PCR enzyme, ÃÖÀûÈ­ µÈ buffer, dNTP Mixture µî)¿Í Àü±â¿µµ¿ ½Ã Æí¸®ÇÑ loading dye (ÆĶõ»ö°ú ³ë¶õ»ö)¸¦ Æ÷ÇÔÇÏ´Â 2¡¿ Premix type PCR È¿¼ÒÀÌ´Ù. Primer¿Í template DNA¹× dH2O¸¦ ÷°¡ÇÏ´Â °ÍÀ¸·Î °£´ÜÈ÷ PCRÀ» ¼öÇà ÇÒ ¼ö ÀÖÀ¸¸ç, ¹ÝÀÀ ÈÄ¿¡´Â ¹ÝÀÀ¾×À» Á÷Á¢ Àü±â¿µµ¿¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ º°µµÀÇ Á¤Á¦ ´Ü°è ¾øÀÌ ¹Ù·Î TA-Cloning À̳ª Sequencing¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
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EmeraldAmp GT PCR Master Mix (2x Premix) 4 x 1§¢
dH2O 4 x 1§¢
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-20¡É (4¡É¿¡¼­ 3°³¿ù º¸Á¸ °¡´É)
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* DNA, cDNAÀÇ PCR ÁõÆø
* Colony PCR µî
Àü±â ¿µµ¿ loading dye
¹ÝÀÀ¾× 5¥ìlÀ» 1% Agarose L03·Î Àü±â¿µµ¿ ÇÏ¿´À» °æ¿ì, ÆĶõ»ö ¸¶Ä¿´Â 3¡­5 kb ºÎ±Ù, ³ë¶õ»ö ¸¶Ä¿´Â 50 bp ÀÌÇÏÀÇ À§Ä¡¿¡¼­ È®ÀÎÇÒ ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ¹ü¿ëÀûÀÎ ¿Ã¶ó¿î´õ agarose: SeaKem LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword : PCR premix,colony PCR,ÄÝ·Î´Ï PCR,cloning,Ŭ·Î´×

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