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Home > ÀüÁ¦Ç°º¸±â > PCR > Ư¼ö PCR (Epigenetics, UNG µî) > EpiTaq HS (for bisulfite-treated DNA)
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû

EpiTaq HS (for bisulfite-treated DNA)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R110A
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
270,400¿ø 
338,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R110A_e.v1910Da.pdf
Takara
R110B
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (R110A x 4)
1,216,000¿ø 

  • Uracil(U)À» ¸¹ÀÌ Æ÷ÇÔÇÑ DNA ÁõÆø¿¡ ÃÖÀû
  • Anti-Taq antibody¸¦ ÀÌ¿ëÇÑ hot-start PCR È¿¼Ò·Î ºñƯÀÌÀû ÁõÆø ¾ïÁ¦
  • GC-rich target, AT-rich target ¸ðµÎ È¿À²ÀûÀ¸·Î ÁõÆø °¡´É
  • ÁõÆø »ê¹°ÀÇ 3£§¸»´Ü¿¡ A°¡ ºÎ°¡, ±×´ë·Î TA Ŭ·Î´×¿¡ »ç¿ë °¡´É
Á¦Ç°¼³¸í
TaKaRa EpiTaq HS (for bisulfite-treated DNA)´Â bisulfite ó¸® ÈÄ UracilÀ» ¸¹ÀÌ Æ÷ÇÔÇÑ DNA¸¦ ÁÖÇüÀ¸·Î bisulfite sequencingÀ̳ª COBRA¹ý µîÀÇ ºÐ¼®À» À§ÇØ PCR ÁõÆø½Ã ÃÖÀûÀÎ PCR È¿¼ÒÀÌ´Ù. Bisulfite 󸮸¦ ÇÑ DNA´Â PCR ¹ÝÀÀ¼º¿¡ º¯È­°¡ ÀÖÀ» ¼ö ÀÖÁö¸¸, º» È¿¼Ò´Â ¸¶±×³×½· ³óµµ³ª dNTP ³óµµ¸¦ Á¶Á¤ÇÏ¿© ÁõÆø È¿À², ¹ÝÀÀ ƯÀ̼ºÀ» Á¶ÀýÇÒ ¼ö Àֱ⠶§¹®¿¡, Áö±Ý±îÁö ÁõÆøÇÒ ¼ö ¾ø¾ú´ø Ÿ°Ù¿¡¼­µµ ¾çÈ£ÇÑ ÁõÆøÀ» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
º» È¿¼Ò´Â anti-Taq antibody¸¦ Æ÷ÇÔÇÑ hot-start PCR È¿¼ÒÀ̸ç, °í¿Â °¡¿­ÇÒ ¶§±îÁö´Â anti-Taq antibody°¡ È¿¼Ò¿¡ °áÇÕÇØ polymerase È°¼ºÀ» ¾ïÁ¦ÇÏ°í Àֱ⠶§¹®¿¡, PCR cycle ÀüÀÇ mis-primmingÀ̳ª primer dimer¿¡¼­ À¯·¡µÇ´Â ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. Anti-Taq antibody´Â PCR Ãʱâ DNA º¯¼º °úÁ¤(initial denaturation)¿¡¼­ º¯¼ºµÇ¾î ºÐ¸®µÇ¹Ç·Î, º°µµÀÇ º¯¼º ½ºÅÜÀ» ¼³Á¤ÇÒ ÇÊ¿ä°¡ ¾ø´Ù.

[ÁÖÀÇ] Bisulfite ó¸®ÇÑ DNA¸¦ ÀÌ¿ëÇÏ¿©, ¸ÞÆ¿È­ ƯÀÌÀû PCR(Methylation Specific PCR: MSP) ºÐ¼®À» ÇÒ °æ¿ì¿¡´Â EpiScope MSP Kit(Code R100A/B)À» Ãßõ ÇÑ´Ù.
³»¿ë (200 rxns)*
1. TaKaRa EpiTaq HS(5 U/¥ìl) 250 U
2. 10¡¿EpiTaq PCR Buffer (Mg2+ free) 1 ml
3. dNTP Mixture(°¢ 2.5 mM) 1.2 ml
4. 25 mM MgCl2 1.2 ml
* ¹ÝÀÀ¿ë·® 50 ¥ìl ±âÁØ

º¸Á¸
- 20¡É
°ü·ÃÁ¦Ç°
- ¹ü¿ëÀûÀÎ ¿Ã¶ó¿î´õ agarose: SeaKem LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword : epigenetics,ÈļºÀ¯ÀüÇÐ,methylation,bisulfite DNA,methylated DNA

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