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PCR Enzyme (Clontech)
> CloneAmp HiFi PCR Premix
High Fidelity PCR È¿¼Ò·Î Cloning¿¡ ÃÖÀû
CloneAmp HiFi PCR Premix
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Clontech
639298
CloneAmp HiFi PCR Premix
200 rxns
281,000¿ø
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Overview
PCR FAQs - ÃßõÇÏ´Â PC..
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High Fidelity È¿¼Ò-Pri..
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µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
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Welchii±Õ °ËÃâ¿ë Prime..
³ôÀº Á¤È®µµ
- ±Øµµ·Î ³·Àº PCR ¿¡·¯À² (12 mismatched bases per 542,580 total bases)
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- priming È¿À²ÀÌ ³ô°í extension (5 sec/kb)ÀÌ °¡´ÉÇØ fast PCR Àû¿ë °¡´É
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- hot start antibody ä¿ëÀ¸·Î nonspecific amplification ¹æÁö
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- ±ä ´ÜÆí(up to 10 kb)À̳ª GC-rich ÁÖÇü ÁõÆø¿¡µµ ÀûÇÕ
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CloneAmp HiFi PCR Premix
´Â CloneAmp HiFi polymerase¿Í dNTP ±×¸®°í buffer°¡ ¸ðµÎ Æ÷ÇÔµÈ Æí¸®ÇÑ 2x master mix Á¦Ç°À¸·Î ³ôÀº Á¤È®µµ »Ó¸¸ ¾Æ´Ï¶ó ¶Ù¾î³ ÁõÆø È¿À²À» Áö´Ñ high fidelity PCRÈ¿¼ÒÀÌ´Ù. ÀÌ È¿¼Ò¸¦ ÀÌ¿ëÇϸé ÃÖ´ë 10kb±îÁö ÁõÆøÇÒ ¼ö ÀÖÀ¸¸ç GC richÀÎ ÁÖÇü¿¡µµ È¿À²ÀûÀ¸·Î ÁõÆøÇÒ ¼ö ÀÖÀ¸¸ç ¿¡·¯À²ÀÌ ¸Å¿ì ³·¾Æ cloningÀ» À§ÇÑ ´ÜÆí ÁõÆø¿¡ ¸Å¿ì ÀÌ»óÀûÀÌ´Ù. ƯÈ÷ In-Fusion PCR Cloning Á¦Ç°°ú ÇÔ²² »ç¿ëÀ» ÃßõÇÑ´Ù.
Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes.
Eight arbitrarily selected GC-rich regions were amplified with CloneAmp HiFi Polymerase or other DNA polymerases using a Thermus thermophilus HB8 genomic DNA template, and cloned into suitable plasmids. Multiple clones were selected for each amplification product and subjected to sequence analysis. DNA fragments amplified using CloneAmp HiFi Polymerase yielded only 12 mismatched bases per 542,580 total bases-higher than an alternative high-fidelity enzyme from Company A, and 10-fold higher than Taq DNA polymerase.
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Keyword : Cloning, pfu, High fidelity, In-fusion, infusion, Ŭ·Î´×, in-fusion cloning, infusion cloning, ÀÎÇ»ÀüŬ·Î´×, ÀÎÇ»Àü
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