´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > Advantage Genomics LA polymerase Mix

Advantage Genomics LA polymerase Mix

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
639152
Advantage Genomic LA Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 rxns
353,600¿ø 
442,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32407
Clontech
639153
Advantage GC Genomic LA Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 rxns
741,600¿ø 
927,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32406

Á¦Ç° ¼³¸í
Advantage Genomic LA Polymerase Mix and Advantage GC Genomic LA Polymerase Mix
- "±æ°í Á¤È®ÇÑ" PCR ÁõÆø
- ¿¡·¯°¡ °ÅÀÇ ¾ø´Â PCR »ê¹°
- ÃÖ´ë 41 kb±îÁö target ÁõÆø
- Advantage GC Genomic LA Polymerase Mix´Â º¹ÀâÇÑ ±¸Á¶¿Í GC-rich genome ÁÖÇü¿¡ ÃÖÀû

Advantage Genomic LA Polymerase Mix ¹× Advantage GC Genomic LA Polymerase Mix´Â ÀϹÝÀûÀÎ PCR È¿¼Ò¿¡¼­´Â ÁõÆøÀÌ ¾î·Á¿î º¹ÀâÇÑ ±¸Á¶³ª GC ÇÔ·®ÀÌ ³ôÀº ÁÖÇü µî ¸Å¿ì ´Ù¾çÇÑ ÁÖÇü¿¡¼­ ±ä ´ÜÆíÀÇ Á¤È®ÇÑ ÁõÆøÀÌ °¡´ÉÇÑ PCR È¿¼ÒÀÌ´Ù.
ÃÖ»óÀÇ È¥ÇÕ È¿¼Ò
µÎ Á¦Ç°Àº ¸ðµÎ Advantage Genomic LA Polymerase¸¦ »ç¿ëÇÑ´Ù. Advantage Genomic LA Polymerase´Â ¿ÏÀüÇÑ ±æÀÌÀÇ ³»¿­¼º Taq DNA polymerase¿Í ¼Ò·®ÀÇ proofreading È¿¼Ò ¹× hot start antibody°¡ Æ÷ÇԵǾî ÀÖ´Ù. Proofreading È¿¼Ò°¡ °¡Áö´Â 3¡Ç¡æ5¡Çexonuclease È°¼ºÀº À߸ø »ðÀÔµÈ ¿°±â¸¦ Á¦°ÅÇÏ°í Áö¼ÓÀûÀ¸·Î ½ÅÀå ¹ÝÀÀÀ» ÁøÇàÇÏ¿© ±ä »ç½½ DNA ´ÜÆíÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Advantage GC Genomic LA Polymerase Mix´Â Clontech °íÀ¯ÀÇ GC-Melt Reagent°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, GC ÇÔ·®ÀÌ ³ôÀº ¼­¿­ÀÇ ÁõÆøÀ» ±ØÀûÀ¸·Î Çâ»ó½ÃŲ´Ù. ÀÌ PCR È¿¼Ò¸¦ ÀÌ¿ëÇϸé human genomic DNA¸¦ ÁÖÇüÀ¸·Î 30 kb±îÁö (GC ÇÔ·®ÀÌ ³ôÀº °æ¿ì´Â 20 kb) ¶ÇÇÑ º¹ÀâÇÏÁö ¾ÊÀº ÀϹÝÀûÀÎ ÁÖÇüÀÇ °æ¿ì 48 kb ÀÌ»óÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù(Figure 1, 3).
³ôÀº Á¤È®¼º & ³ôÀº ÁõÆøÈ¿À²
3¡Ç¡æ5¡Çexonuclease È°¼ºÀ» °¡Áö´Â ÀÌ È¥ÇÕ È¿¼Ò´Â wild-type Taq DNA Poly meraseº¸´Ù 6.5 ¹è ´õ ³ôÀº Á¤È®¼ºÀ» ³ªÅ¸³½´Ù (internal data´Â reference 1 ¿¡¼­ ¾ò¾ú´Ù). ÀÌ °­·ÂÇÑ È¿¼Ò ½Ã½ºÅÛÀº background¸¦ ÃÖ¼ÒÈ­Çϸç, ¸Å¿ì ³ôÀº ÁõÆøÈ¿À²À» º¸ÀåÇÏ°í, ´Ù¸¥ Á¾·ùÀÇ "long and accurate" polymerase¿¡ ºñÇÏ¿© PCR Á¶°Ç °ËÅä°¡ ¿ëÀÌÇÏ´Ù. ¶ÇÇÑ, µÎ Á¦Ç°¿¡ ÷ºÎµÈ ¹öÆÛ´Â ³ôÀº ÁõÆøÈ¿À²°ú Á¤È®¼ºÀ» Çâ»ó½ÃÅ°´Âµ¥ ÃÖÀûÈ­µÇ¾î ÀÖ´Ù.
¶Ù¾î³­ ¹ÝÀÀ ƯÀ̼º
Hot start antibody´Â ½Ç¿Â¿¡¼­´Â DNA polymerase È°¼ºÀ» ÀúÇØÇÏ¿© ÀÚµ¿À¸·Î hot start PCRÀ» ÇÒ ¼ö ÀÖ´Ù. PCR Ãʱ⠺¯¼º °úÁ¤¿¡¼­ antibody°¡ º¯¼ºµÇ¸é polymeraseÀÇ È°¼ºÀÌ º¹¿øµÇ¸ç, hot start antibody´Â PCR ½ÃÀÛ Àü¿¡ ºñƯÀÌÀû ÁõÆø ¹× À߸øµÈ primer »ê¹°À» °¨¼Ò½ÃÅ°°Å³ª Á¦°ÅÇÏ¿© PCR È¿À²°ú ¹ÝÀÀ ƯÀ̼ºÀ» Å©°Ô Çâ»ó½ÃŲ´Ù.
¶ÇÇÑ, Advantage GC Genomic LA Polymerase Mix·Î ÁõÆøµÈ PCR »ê¹°µéÀº 3¡Ç¡æA µ¹ÃâÀÌ ÀÖ¾î TA-vector¿¡ cloningÀÌ °¡´ÉÇÏ´Ù.

* ÀÚ¼¼ÇÑ ³»¿ë/±×¸² µîÀº [more] ¸¦ ÅëÇØ È®ÀÎ °¡´ÉÇÕ´Ï´Ù.

Keyword :

PCR Enzyme (Clontech)
>
ClontechÀÇ µ¶ÀÚÀûÀÎ blend Çü È¿¼Ò
Advantage 2 Polymerase Mix & PCR Kit
>
Super accuracy, high specificity
Advantage HD Polymerase Mix
>
High Fidelity PCR È¿¼Ò·Î Cloning¿¡ ÃÖÀû
CloneAmp HiFi PCR Premix
>
³ôÀº fidelity¿Í ÁõÆøÈ¿À²À» °âºñÇÑ µ¿°á°ÇÁ¶µÈ..
High Fidelity PCR EcoDry Premix
>
³ôÀº ÁõÆø È¿À²ÀÇ µ¿°á°ÇÁ¶µÈ PCR mastermix
High Yield PCR EcoDry Premix
>
ºü¸£°í ±ÕÀÏÇÑ Multiplexing PCRÀ» À§ÇÑ 2X PCR..
SuperPlex¢â Premix
>
Direct PCR È¿¼Ò
Terra PCR Direct Polymerase Mix
>
5£§-exonuclease È°¼ºÀ» Á¦°ÅÇÑ PCR È¿¼Ò
TITANIUM¢ç Taq DNA Polymerase