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PCR Enzyme (Clontech)
> Advantage Genomics LA polymerase Mix
Advantage Genomics LA polymerase Mix
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Clontech
639152
Advantage Genomic LA Polymerase Mix
100 rxns
442,000¿ø
Clontech
639153
Advantage GC Genomic LA Polymerase Mix
200 rxns
927,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
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Advantage Genomic LA Polymerase Mix and Advantage GC Genomic LA Polymerase Mix
- "±æ°í Á¤È®ÇÑ" PCR ÁõÆø
- ¿¡·¯°¡ °ÅÀÇ ¾ø´Â PCR »ê¹°
- ÃÖ´ë 41 kb±îÁö target ÁõÆø
- Advantage GC Genomic LA Polymerase Mix´Â º¹ÀâÇÑ ±¸Á¶¿Í GC-rich genome ÁÖÇü¿¡ ÃÖÀû
Advantage Genomic LA Polymerase Mix ¹× Advantage GC Genomic LA Polymerase Mix´Â ÀϹÝÀûÀÎ PCR È¿¼Ò¿¡¼´Â ÁõÆøÀÌ ¾î·Á¿î º¹ÀâÇÑ ±¸Á¶³ª GC ÇÔ·®ÀÌ ³ôÀº ÁÖÇü µî ¸Å¿ì ´Ù¾çÇÑ ÁÖÇü¿¡¼ ±ä ´ÜÆíÀÇ Á¤È®ÇÑ ÁõÆøÀÌ °¡´ÉÇÑ PCR È¿¼ÒÀÌ´Ù.
ÃÖ»óÀÇ È¥ÇÕ È¿¼Ò
µÎ Á¦Ç°Àº ¸ðµÎ Advantage Genomic LA Polymerase¸¦ »ç¿ëÇÑ´Ù. Advantage Genomic LA Polymerase´Â ¿ÏÀüÇÑ ±æÀÌÀÇ ³»¿¼º Taq DNA polymerase¿Í ¼Ò·®ÀÇ proofreading È¿¼Ò ¹× hot start antibody°¡ Æ÷ÇԵǾî ÀÖ´Ù. Proofreading È¿¼Ò°¡ °¡Áö´Â 3¡Ç¡æ5¡Çexonuclease È°¼ºÀº À߸ø »ðÀÔµÈ ¿°±â¸¦ Á¦°ÅÇÏ°í Áö¼ÓÀûÀ¸·Î ½ÅÀå ¹ÝÀÀÀ» ÁøÇàÇÏ¿© ±ä »ç½½ DNA ´ÜÆíÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Advantage GC Genomic LA Polymerase Mix´Â Clontech °íÀ¯ÀÇ GC-Melt Reagent°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, GC ÇÔ·®ÀÌ ³ôÀº ¼¿ÀÇ ÁõÆøÀ» ±ØÀûÀ¸·Î Çâ»ó½ÃŲ´Ù. ÀÌ PCR È¿¼Ò¸¦ ÀÌ¿ëÇϸé human genomic DNA¸¦ ÁÖÇüÀ¸·Î 30 kb±îÁö (GC ÇÔ·®ÀÌ ³ôÀº °æ¿ì´Â 20 kb) ¶ÇÇÑ º¹ÀâÇÏÁö ¾ÊÀº ÀϹÝÀûÀÎ ÁÖÇüÀÇ °æ¿ì 48 kb ÀÌ»óÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù(Figure 1, 3).
³ôÀº Á¤È®¼º & ³ôÀº ÁõÆøÈ¿À²
3¡Ç¡æ5¡Çexonuclease È°¼ºÀ» °¡Áö´Â ÀÌ È¥ÇÕ È¿¼Ò´Â wild-type Taq DNA Poly meraseº¸´Ù 6.5 ¹è ´õ ³ôÀº Á¤È®¼ºÀ» ³ªÅ¸³½´Ù (internal data´Â reference 1 ¿¡¼ ¾ò¾ú´Ù). ÀÌ °·ÂÇÑ È¿¼Ò ½Ã½ºÅÛÀº background¸¦ ÃÖ¼ÒÈÇϸç, ¸Å¿ì ³ôÀº ÁõÆøÈ¿À²À» º¸ÀåÇÏ°í, ´Ù¸¥ Á¾·ùÀÇ "long and accurate" polymerase¿¡ ºñÇÏ¿© PCR Á¶°Ç °ËÅä°¡ ¿ëÀÌÇÏ´Ù. ¶ÇÇÑ, µÎ Á¦Ç°¿¡ ÷ºÎµÈ ¹öÆÛ´Â ³ôÀº ÁõÆøÈ¿À²°ú Á¤È®¼ºÀ» Çâ»ó½ÃÅ°´Âµ¥ ÃÖÀûȵǾî ÀÖ´Ù.
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Hot start antibody´Â ½Ç¿Â¿¡¼´Â DNA polymerase È°¼ºÀ» ÀúÇØÇÏ¿© ÀÚµ¿À¸·Î hot start PCRÀ» ÇÒ ¼ö ÀÖ´Ù. PCR Ãʱ⠺¯¼º °úÁ¤¿¡¼ antibody°¡ º¯¼ºµÇ¸é polymeraseÀÇ È°¼ºÀÌ º¹¿øµÇ¸ç, hot start antibody´Â PCR ½ÃÀÛ Àü¿¡ ºñƯÀÌÀû ÁõÆø ¹× À߸øµÈ primer »ê¹°À» °¨¼Ò½ÃÅ°°Å³ª Á¦°ÅÇÏ¿© PCR È¿À²°ú ¹ÝÀÀ ƯÀ̼ºÀ» Å©°Ô Çâ»ó½ÃŲ´Ù.
¶ÇÇÑ, Advantage GC Genomic LA Polymerase Mix·Î ÁõÆøµÈ PCR »ê¹°µéÀº 3¡Ç¡æA µ¹ÃâÀÌ ÀÖ¾î TA-vector¿¡ cloningÀÌ °¡´ÉÇÏ´Ù.
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Keyword :
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±Åà °¡À̵å
[°³¿ä] So Easy Product
[Cancer Research] Cancer biomarker quantification
ClontechÀÇ µ¶ÀÚÀûÀÎ blend Çü È¿¼Ò
Advantage 2 Polymerase Mix & PCR Kit
Advantage GC 2 Polymerase Mix & PCR Kits
Advantage Genomics LA polymerase Mix
Super accuracy, high specificity
Advantage HD Polymerase Mix
Advantage HF 2 PCR Kit
High Fidelity PCR È¿¼Ò·Î Cloning¿¡ ÃÖÀû
CloneAmp HiFi PCR Premix
³ôÀº fidelity¿Í ÁõÆøÈ¿À²À» °âºñÇÑ µ¿°á°ÇÁ¶µÈ..
High Fidelity PCR EcoDry Premix
³ôÀº ÁõÆø È¿À²ÀÇ µ¿°á°ÇÁ¶µÈ PCR mastermix
High Yield PCR EcoDry Premix
SeqAmp¢â DNA Polymerase
ºü¸£°í ±ÕÀÏÇÑ Multiplexing PCRÀ» À§ÇÑ 2X PCR..
SuperPlex¢â Premix
Direct PCR È¿¼Ò
Terra PCR Direct Polymerase Mix
5£§-exonuclease È°¼ºÀ» Á¦°ÅÇÑ PCR È¿¼Ò
TITANIUM¢ç Taq DNA Polymerase