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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > Advantage Genomics LA polymerase Mix

Advantage Genomics LA polymerase Mix

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Clontech
639152
Advantage Genomic LA Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 rxns
442,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32407
Clontech
639153
Advantage GC Genomic LA Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 rxns
927,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32406

Á¦Ç° ¼³¸í
Advantage Genomic LA Polymerase Mix and Advantage GC Genomic LA Polymerase Mix
- "±æ°í Á¤È®ÇÑ" PCR ÁõÆø
- ¿¡·¯°¡ °ÅÀÇ ¾ø´Â PCR »ê¹°
- ÃÖ´ë 41 kb±îÁö target ÁõÆø
- Advantage GC Genomic LA Polymerase Mix´Â º¹ÀâÇÑ ±¸Á¶¿Í GC-rich genome ÁÖÇü¿¡ ÃÖÀû

Advantage Genomic LA Polymerase Mix ¹× Advantage GC Genomic LA Polymerase Mix´Â ÀϹÝÀûÀÎ PCR È¿¼Ò¿¡¼­´Â ÁõÆøÀÌ ¾î·Á¿î º¹ÀâÇÑ ±¸Á¶³ª GC ÇÔ·®ÀÌ ³ôÀº ÁÖÇü µî ¸Å¿ì ´Ù¾çÇÑ ÁÖÇü¿¡¼­ ±ä ´ÜÆíÀÇ Á¤È®ÇÑ ÁõÆøÀÌ °¡´ÉÇÑ PCR È¿¼ÒÀÌ´Ù.
ÃÖ»óÀÇ È¥ÇÕ È¿¼Ò
µÎ Á¦Ç°Àº ¸ðµÎ Advantage Genomic LA Polymerase¸¦ »ç¿ëÇÑ´Ù. Advantage Genomic LA Polymerase´Â ¿ÏÀüÇÑ ±æÀÌÀÇ ³»¿­¼º Taq DNA polymerase¿Í ¼Ò·®ÀÇ proofreading È¿¼Ò ¹× hot start antibody°¡ Æ÷ÇԵǾî ÀÖ´Ù. Proofreading È¿¼Ò°¡ °¡Áö´Â 3¡Ç¡æ5¡Çexonuclease È°¼ºÀº À߸ø »ðÀÔµÈ ¿°±â¸¦ Á¦°ÅÇÏ°í Áö¼ÓÀûÀ¸·Î ½ÅÀå ¹ÝÀÀÀ» ÁøÇàÇÏ¿© ±ä »ç½½ DNA ´ÜÆíÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Advantage GC Genomic LA Polymerase Mix´Â Clontech °íÀ¯ÀÇ GC-Melt Reagent°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, GC ÇÔ·®ÀÌ ³ôÀº ¼­¿­ÀÇ ÁõÆøÀ» ±ØÀûÀ¸·Î Çâ»ó½ÃŲ´Ù. ÀÌ PCR È¿¼Ò¸¦ ÀÌ¿ëÇϸé human genomic DNA¸¦ ÁÖÇüÀ¸·Î 30 kb±îÁö (GC ÇÔ·®ÀÌ ³ôÀº °æ¿ì´Â 20 kb) ¶ÇÇÑ º¹ÀâÇÏÁö ¾ÊÀº ÀϹÝÀûÀÎ ÁÖÇüÀÇ °æ¿ì 48 kb ÀÌ»óÀÇ ÁõÆøÀÌ °¡´ÉÇÏ´Ù(Figure 1, 3).
³ôÀº Á¤È®¼º & ³ôÀº ÁõÆøÈ¿À²
3¡Ç¡æ5¡Çexonuclease È°¼ºÀ» °¡Áö´Â ÀÌ È¥ÇÕ È¿¼Ò´Â wild-type Taq DNA Poly meraseº¸´Ù 6.5 ¹è ´õ ³ôÀº Á¤È®¼ºÀ» ³ªÅ¸³½´Ù (internal data´Â reference 1 ¿¡¼­ ¾ò¾ú´Ù). ÀÌ °­·ÂÇÑ È¿¼Ò ½Ã½ºÅÛÀº background¸¦ ÃÖ¼ÒÈ­Çϸç, ¸Å¿ì ³ôÀº ÁõÆøÈ¿À²À» º¸ÀåÇÏ°í, ´Ù¸¥ Á¾·ùÀÇ "long and accurate" polymerase¿¡ ºñÇÏ¿© PCR Á¶°Ç °ËÅä°¡ ¿ëÀÌÇÏ´Ù. ¶ÇÇÑ, µÎ Á¦Ç°¿¡ ÷ºÎµÈ ¹öÆÛ´Â ³ôÀº ÁõÆøÈ¿À²°ú Á¤È®¼ºÀ» Çâ»ó½ÃÅ°´Âµ¥ ÃÖÀûÈ­µÇ¾î ÀÖ´Ù.
¶Ù¾î³­ ¹ÝÀÀ ƯÀ̼º
Hot start antibody´Â ½Ç¿Â¿¡¼­´Â DNA polymerase È°¼ºÀ» ÀúÇØÇÏ¿© ÀÚµ¿À¸·Î hot start PCRÀ» ÇÒ ¼ö ÀÖ´Ù. PCR Ãʱ⠺¯¼º °úÁ¤¿¡¼­ antibody°¡ º¯¼ºµÇ¸é polymeraseÀÇ È°¼ºÀÌ º¹¿øµÇ¸ç, hot start antibody´Â PCR ½ÃÀÛ Àü¿¡ ºñƯÀÌÀû ÁõÆø ¹× À߸øµÈ primer »ê¹°À» °¨¼Ò½ÃÅ°°Å³ª Á¦°ÅÇÏ¿© PCR È¿À²°ú ¹ÝÀÀ ƯÀ̼ºÀ» Å©°Ô Çâ»ó½ÃŲ´Ù.
¶ÇÇÑ, Advantage GC Genomic LA Polymerase Mix·Î ÁõÆøµÈ PCR »ê¹°µéÀº 3¡Ç¡æA µ¹ÃâÀÌ ÀÖ¾î TA-vector¿¡ cloningÀÌ °¡´ÉÇÏ´Ù.

* ÀÚ¼¼ÇÑ ³»¿ë/±×¸² µîÀº [more] ¸¦ ÅëÇØ È®ÀÎ °¡´ÉÇÕ´Ï´Ù.

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