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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Subtraction °ü·Ã Á¦Ç° > Clontech PCR-Select Differencial Screening Kit
Substraction ÀüÈÄ cDNA ¹ßÇö Â÷À̸¦ °£´ÜÇÏ°Ô È®ÀÎ

Clontech PCR-Select Differencial Screening Kit

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Clontech
637403
Clontech PCR-Select Differential Screening Kit
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1 Set
1,223,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32590, x99386
Clontech
637402
Clontech PCR-Select Differential Screening Blocking Solution
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml
646,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

Clontech PCR-select differential screening KitÀ» »ç¿ëÇϸé subtractionÀÌ ³¡³­ library Áß¿¡¼­ Á¤¸»·Î ƯÀÌÀû ¹ßÇöÇÏ°í ÀÖ´Â cDNA¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù. Clontech PCR-select cDNA subtraction KitÀ¸·Î ¹ßÇö·®¿¡ Â÷ÀÌ°¡ ÀÖ´Â À¯ÀüÀÚ poolÀ» ¸¸µç ÈÄ, º» KitÀ» »ç¿ëÇÏ¸é ±× ¹ßÇöÂ÷¸¦ °£ÆíÇÏ°Ô È®ÀÎÇÒ ¼ö ÀÖ´Ù.
PCR-select differential screening¿¡¼­´Â subtractionÀÌ ³¡³­ library¿¡ ´ëÇØ tester ¹× driver RNA·Î Á÷Á¢ ÇÕ¼ºÇÑ probe, forward subtractionÀÌ ³¡³­ cDNA·Î Á¦ÀÛÇÑ probe ¹× reverse subtractionÀÌ ³¡³­ cDNA(2 ȸ°ÀÇ reverse-subtracted)·Î Á¦ÀÛÇÑ probe¸¦ »ç¿ëÇÏ¿© hybridization½ÃÄ×´Ù. ƯÀÌÀû ¹ßÇö cloneÀº driver¿Í´Â hybridization½ÃÅ°Áö ¾Ê°í tester¸¸À¸·Î hybridization½ÃÅ°´Âµ¥, subtraction ÀüÀÇ probe¿¡¼­´Â °¨µµ°¡ ³·±â ¶§¹®¿¡ º»·¡ÀÇ Á¤º¸¸¦ °ËÃâÇÒ ¼ö ¾ø´Ù. ¹Ý¸é subtractionÀÌ ³¡³­ probe¿¡´Â ƯÀÌÀû ¹ßÇö cDNA°¡ »ó´çÈ÷ ³óÃàµÇ¾î Àִµ¥, °á°ú°¡ false positive¸¦ ³ªÅ¸³»´Â °æ¿ì°¡ ÀÖ´Ù. º» ¹æ¹ý¿¡¼­´Â subtraction ÀüÈÄÀÇ ¾ç probe(subtracted ¿Í non-subtracted probes)¸¦ »ç¿ëÇÔÀ¸·Î½á ÃÖ°í È¿À²·Î ƯÀÌÀû ¹ßÇö À¯ÀüÀÚÀÇ µ¿Á¤À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.

PCR-select differential screening Kit¿¡´Â subtraction ÀüÈÄÀÇ ¾ç probe Á¦ÀÛ¿¡ ÇÊ¿äÇÑ ½Ã¾à, control ¹× user manualÀÌ Ã·ºÎµÇ¾î ÀÖ´Ù. NA polymerase mix´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. °í°¨µµÀÌ°í Á¤È®ÇÑ PCRÀ» À§ÇØ advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.

±×¸² 1. Clontech PCR-select differential screening¿¡ ÀÇÇÑ Èñ¼ÒÇÑ Æ¯ÀÌÀû ¹ßÇö cDNAÀÇ °ËÃâ SMART PCR cDNA synthesis Kit(Cat. No. 634902)À» »ç¿ëÇÏ¿© ¥ã-globin »ý¼º ¼¼Æ÷ÁÖ ¹× ¥â-globin »ý¼º ¼¼Æ÷ÁÖ·Î total RNAÀÇ ¿¹ºñÁõÆøÀ» ½Ç½ÃÇÏ¿´´Ù. ¥ãÁÖ À¯·¡ÀÇ cDNA¸¦ tester, ¥âÁÖ À¯·¡ÀÇ cDNA¸¦ driver¿¡ »ç¿ëÇÏ¿© PCR-select cDNA subtractionÀ» ½Ç½ÃÇÏ¿´´Ù. Reverse subtractionÀº tester¿Í driver¸¦ ¹Ù²Ù¾î ³Ö¾î ½Ç½ÃÇÏ¿´´Ù. À̾ subtractionÀÌ ³¡³­ cDNA¸¦ cloneÈ­ÇÏ°í ¹«ÀÛÀ§·Î ¼±ÅÃÇÑ cloneÀ» °¢ nylon membraneÀÇ µ¿ÀÏÀ§Ä¡¿¡ plotÇÏ¿´´Ù. MembraneÀº ±×¸² ¼Ó¿¡ ³ªÅ¸³½ probe¸¦ »ç¿ëÇÏ¿© hybrid½ÃÄ×´Ù.
±¸¼º¹°
  • Nested primer 1
  • Nested primer 2R
  • Reaction buffer(-dCTP)
  • Reaction buffer(-dATP)
  • Random primer mix
  • Klenow enzyme(exo-)
  • Negative hybridization control(cDNA 1R)
  • Negative hybridization control(cDNA 2R)
  • Blocking solution
  • Stop solution
  • User manual(PT3138-1)
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Keyword :

PCR Subtraction °ü·Ã Á¦Ç°
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Clontech PCR-Select cDNA Subtraction Kit
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Substraction ÀüÈÄ cDNA ¹ßÇö Â÷À̸¦ °£´ÜÇÏ°Ô ..
Clontech PCR-Select Differencial Screening Kit