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PCR Subtraction °ü·Ã Á¦Ç°
> Clontech PCR-Select Differencial Screening Kit
Substraction ÀüÈÄ cDNA ¹ßÇö Â÷À̸¦ °£´ÜÇÏ°Ô È®ÀÎ
Clontech PCR-Select Differencial Screening Kit
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Clontech
637403
Clontech PCR-Select Differential Screening Kit
1 Set
1,223,000¿ø
,
Clontech
637402
Clontech PCR-Select Differential Screening Blocking Solution
1 ml
646,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Clontech PCR-select differential screening KitÀ» »ç¿ëÇϸé subtractionÀÌ ³¡³ library Áß¿¡¼ Á¤¸»·Î ƯÀÌÀû ¹ßÇöÇÏ°í ÀÖ´Â cDNA¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù. Clontech PCR-select cDNA subtraction KitÀ¸·Î ¹ßÇö·®¿¡ Â÷ÀÌ°¡ ÀÖ´Â À¯ÀüÀÚ poolÀ» ¸¸µç ÈÄ, º» KitÀ» »ç¿ëÇÏ¸é ±× ¹ßÇöÂ÷¸¦ °£ÆíÇÏ°Ô È®ÀÎÇÒ ¼ö ÀÖ´Ù.
PCR-select differential screening¿¡¼´Â subtractionÀÌ ³¡³ library¿¡ ´ëÇØ tester ¹× driver RNA·Î Á÷Á¢ ÇÕ¼ºÇÑ probe, forward subtractionÀÌ ³¡³ cDNA·Î Á¦ÀÛÇÑ probe ¹× reverse subtractionÀÌ ³¡³ cDNA(2 ȸ°ÀÇ reverse-subtracted)·Î Á¦ÀÛÇÑ probe¸¦ »ç¿ëÇÏ¿© hybridization½ÃÄ×´Ù. ƯÀÌÀû ¹ßÇö cloneÀº driver¿Í´Â hybridization½ÃÅ°Áö ¾Ê°í tester¸¸À¸·Î hybridization½ÃÅ°´Âµ¥, subtraction ÀüÀÇ probe¿¡¼´Â °¨µµ°¡ ³·±â ¶§¹®¿¡ º»·¡ÀÇ Á¤º¸¸¦ °ËÃâÇÒ ¼ö ¾ø´Ù. ¹Ý¸é subtractionÀÌ ³¡³ probe¿¡´Â ƯÀÌÀû ¹ßÇö cDNA°¡ »ó´çÈ÷ ³óÃàµÇ¾î Àִµ¥, °á°ú°¡ false positive¸¦ ³ªÅ¸³»´Â °æ¿ì°¡ ÀÖ´Ù. º» ¹æ¹ý¿¡¼´Â subtraction ÀüÈÄÀÇ ¾ç probe(subtracted ¿Í non-subtracted probes)¸¦ »ç¿ëÇÔÀ¸·Î½á ÃÖ°í È¿À²·Î ƯÀÌÀû ¹ßÇö À¯ÀüÀÚÀÇ µ¿Á¤À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
PCR-select differential screening Kit¿¡´Â subtraction ÀüÈÄÀÇ ¾ç probe Á¦ÀÛ¿¡ ÇÊ¿äÇÑ ½Ã¾à, control ¹× user manualÀÌ Ã·ºÎµÇ¾î ÀÖ´Ù. NA polymerase mix´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. °í°¨µµÀÌ°í Á¤È®ÇÑ PCRÀ» À§ÇØ advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
±×¸² 1. Clontech PCR-select differential screening¿¡ ÀÇÇÑ Èñ¼ÒÇÑ Æ¯ÀÌÀû ¹ßÇö cDNAÀÇ °ËÃâ SMART PCR cDNA synthesis Kit(Cat. No. 634902)À» »ç¿ëÇÏ¿© ¥ã-globin »ý¼º ¼¼Æ÷ÁÖ ¹× ¥â-globin »ý¼º ¼¼Æ÷ÁÖ·Î total RNAÀÇ ¿¹ºñÁõÆøÀ» ½Ç½ÃÇÏ¿´´Ù. ¥ãÁÖ À¯·¡ÀÇ cDNA¸¦ tester, ¥âÁÖ À¯·¡ÀÇ cDNA¸¦ driver¿¡ »ç¿ëÇÏ¿© PCR-select cDNA subtractionÀ» ½Ç½ÃÇÏ¿´´Ù. Reverse subtractionÀº tester¿Í driver¸¦ ¹Ù²Ù¾î ³Ö¾î ½Ç½ÃÇÏ¿´´Ù. ÀÌ¾î¼ subtractionÀÌ ³¡³ cDNA¸¦ cloneÈÇÏ°í ¹«ÀÛÀ§·Î ¼±ÅÃÇÑ cloneÀ» °¢ nylon membraneÀÇ µ¿ÀÏÀ§Ä¡¿¡ plotÇÏ¿´´Ù. MembraneÀº ±×¸² ¼Ó¿¡ ³ªÅ¸³½ probe¸¦ »ç¿ëÇÏ¿© hybrid½ÃÄ×´Ù.
±¸¼º¹°
Nested primer 1
Nested primer 2R
Reaction buffer(-dCTP)
Reaction buffer(-dATP)
Random primer mix
Klenow enzyme(exo-)
Negative hybridization control(cDNA 1R)
Negative hybridization control(cDNA 2R)
Blocking solution
Stop solution
User manual(PT3138-1)
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Keyword :
PCR Subtraction °ü·Ã Á¦Ç°
¼¼±Õ GenomeÀ» È¿À²ÀûÀ¸·Î ºñ±³ÇÒ ¼ö ÀÖ´Â Kit
Clontech PCR-Select Bacterial Genome Subtraction Kit
½Å±Ô À¯ÀüÀÚ ¶Ç´Â ¹ßÇö·®¿¡ Â÷ÀÌ°¡ ÀÖ´Â À¯ÀüÀÚ..
Clontech PCR-Select cDNA Subtraction Kit
Substraction ÀüÈÄ cDNA ¹ßÇö Â÷À̸¦ °£´ÜÇÏ°Ô ..
Clontech PCR-Select Differencial Screening Kit