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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > TITANIUM¢ç Taq DNA Polymerase
5£§-exonuclease È°¼ºÀ» Á¦°ÅÇÑ PCR È¿¼Ò

TITANIUM¢ç Taq DNA Polymerase

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
639208
TITANIUM¢ç Taq DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
272,000¿ø 
340,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33156
Clontech
639209
TITANIUM¢ç Taq DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ȸ
1,124,800¿ø 
1,406,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639242
TITANIUM¢ç Taq DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 ȸ
1,932,000¿ø 
2,415,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639211
TITANIUM¢ç Taq PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
30 ȸ
260,800¿ø 
326,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33157, x33156
Clontech
639210
TITANIUM¢ç Taq PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
516,800¿ø 
646,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639141
10¡¿ TITANIUM¢ç Taq PCR Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
600 ¥ìl ¡¿ 2
144,000¿ø 
180,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639142
10¡¿ TITANIUM¢ç Taq PCR Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
294,400¿ø 
368,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

Á¦Ç°¼³¸í
TITANIUM¢ç Taq DNA polymerase
³ôÀº ÁõÆø·®°ú ¶Ù¾î³­ ÀçÇö¼º
- ±Ø¼Ò·®ÀÇ template·Î ½ÇÇèÀ» ÁøÇà ÇÒ °æ¿ì ÀûÇÕ
- hot start ¹æ½Ä-multiplex¿¡ ÃÖÀû
- Mg2+ÀÇ ÃÖÀûÈ­¿Í TaqÀÇ È°¼ºÈ­¸¦ À§ÇÑ 10ºÐ°£ÀÇ cycle Àü󸮰¡ ºÒÇÊ¿ä
TITANIUM¢ç Taq DNA polymerase´Â °í°¨µµÀÌ¸ç °­·ÂÇÑ DNA ÇÕ¼º È¿¼Ò·Î, ÀÏ»óÀûÀÎ ¸ðµç PCR ¿ëµµ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. TITANIUM¢ç Taq´Â bacterial DNA, plasmid DNA, cDNA ±×¸®°í º¹ÇÕÀûÀÎ genome DNA¸¦ Æ÷ÇÔÇÑ ¸ðµç DNA template¿¡¼­ fragment¸¦ È¿À²ÀûÀ¸·Î ÁõÆøÇÑ´Ù(±×¸² 1). ¶ÇÇÑ TITANIUM¢ç Taq¿¡´Â TaqStart antibody°¡ Æ÷ÇԵǾî ÀÖ¾î ƯÀ̼º°ú ÁõÆø·® Çâ»óÀ» º¸ÀδÙ.
PCR performanceÀÇ Çâ»ó
TITANIUM¢ç Taq DNA polymerase´Â Taq polymeraseÀÇ N¸»´Ü °á½Ç mutant´Ù. ÀÌ °á½Ç¿¡ ÀÇÇØ 5'-exonuclease È°¼ºÀÌ Á¦°ÅµÇ¸ç È¿¼ÒÀÇ °¡¿ë¼ºµµ ³ô¾ÆÁø´Ù. º» Á¦Ç°Àº ÇöÀç ÀÌ¿ëÇÒ ¼ö ÀÖ´Â ½ÃÁß ÆǸŠÁ¦Ç° Áß¿¡¼­ °¡Àå °­·ÂÇÏ°í °í°¨µµÀÎ PCR polymerase¶ó°í ÇÒ ¼ö ÀÖ´Ù.
´Ù¸¥ polymeraseº¸´Ù ¶Ù¾î³­ Á¡
- º¸´Ù ÀûÀº PCR cycle·Î targetÀ» ÁõÆø- cycle ¼ö°¡ Àû¾îµµ µÇ¹Ç·Î ½ÇÇè½Ã°£ÀÌ ´ÜÃàµÇ°í backgroundµµ ¾ïÁ¦µÈ´Ù.
- ºÒ°ú 2ºÐÀÚÀÇ ÁõÆø targetµµ °ËÃâ °¡´É- TITANIUM¢ç TaqÀÇ ¶Ù¾î³­ °¨µµ´Â ´Ù¸¥ polymerase¿¡¼­´Â ºÒ°¡´ÉÇÑ target °ËÃâµµ °¡´ÉÇÏ°Ô ÇÑ´Ù.
- ´Ù¾çÇÑ template¿¡¼­ ÃÖ´ë 2 kbÀÇ DNA fragment¸¦ ÁõÆø- ÀϹÝÀûÀÎ Á¸Àç·®ÀÎ cDNA³ª ´Ü¼øÇÑ ±¸Á¶ÀÇ genome DNA¸¦ ÁõÆø ÇÒ °æ¿ì, 4 kb Á¤µµ±îÁöÀÇ fragment¸¦ ½±°Ô ÁõÆøÇÒ ¼ö ÀÖ´Ù.
- buffer ÃÖÀûÈ­ °úÁ¤ÀÌ ÇÊ¿ä ¾øÀ½- TITANIUM¢ç Taq´Â Ÿ»çÀÇ Taq¿¡ ºñÇØ ÈξÀ ±¤¹üÀ§ÇÑ Mg2+ ³óµµ¸¦ Çã¿ëÇϹǷΠº°µµÀÇ buffer ÃÖÀûÈ­°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù.


±×¸²1 TITANIUM¢ç TaqÀº genome DNA¸¦ È¿À²ÀûÀ¸·Î ÁõÆøÇÑ´Ù.
TITANIUM¢ç Taq°ú 2°³ÀÇ Å¸»ç Taq polymerase¸¦ ÀÌ¿ëÇÏ¿©, 100 ngÀÇ genome DNA·ÎºÎÅÍ ´Ù¸¥ ±æÀÌÀÇ human cardiac ¥â-myosin heavy chain fragment¸¦ ÁõÆøÇß´Ù. ¹ÝÀÀ Á¶°ÇÀº °¢ È¿¼ÒÀÇ ÁöÁ¤ Á¶°ÇÀ¸·Î ÁõÆøÇÏ¿´´Ù. Lane 1: 0.5 kb fragment, Lane 2£º1 kb fragment, Lane 3£º1.8 kb fragment, Lane M£º1-kb DNA size ladder
TITANIUM¢ç Taq PCR Kit ±¸¼º¹°
TITANIUM¢ç Taq DNA Polymerase
TITANIUM¢ç Taq PCR Buffer
dNTP Mix (10 mM each)
Control DNA Template
Control Primer Mix
PCR-grade H2O
User Manual (PT3304-1)
Protocol-at-a-Glance (PT3304-2C)
TITANIUM¢ç Taq DNA Polymerase ±¸¼º¹°
TITANIUM¢ç Taq DNA Polymerase
TITANIUM¢ç Taq PCR Buffer
Protocol-at-a-Glance (PT3304-2C)
º¸Á¸ -20¡É
Àû¿ëºÐ¾ß
- Genotyping
- Multiplex PCR
- Primer extension
- Hot-start PCR
- ÀϹÝÀûÀÎ PCR
- ¹Ì·®ÀÇ template

OEM ¶Ç´Â Á¦Ç°ÀÇ ¿øÀç·á·Î »ç¿ë °¡´ÉÇÑ ÇϱâÀÇ Á¦Ç°À» ±¸¸ÅÇϽ÷Á¸é ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (TEL. 02-2081-2510 / mail. support@takara.co.kr)·Î ¿¬¶ô ºÎŹµå¸³´Ï´Ù.
[°ü·Ã Á¦Ç°¸®½ºÆ®]

Code

Á¦Ç°¸í

¿ë·®

639292

Titanium¢ç Taq SP DNA Polymerase

100 rxns

638517

50X Titanium¢ç Taq SP (Glycerol-Free)

250 ul

638519

Titanium¢ç Taq SP EcoDry¢â Premix  

48 rxns


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TITANIUM¢ç Taq DNA Polymerase