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Pyrobest DNA Polymerase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R005A
Pyrobest¢â DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
125 U
165,000¿ø  R005A_DS.v1103Da.pdf
Takara
R005B
Pyrobest¢â DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 U (R005A x 4)
596,000¿ø 


È¿À²ÀûÀÎ PCR ÁõÆøÀ» À§ÇÑ Pyrobest Á¶°Ç¼³Á¤°¡À̵å click!
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº Pyrococcus sp. À¯·¡ÀÇ 3¡¯¡æ 5¡¯ exonuclease È°¼º(proof reading È°¼º)À» °®´Â ³»¿­¼º ¥áÇü DNA polymeraseÀÌ´Ù. Pyrococcus furiosus À¯·¡ÀÇ Pfu DNA polymerase³ª Vent DNA polymerase¿Í °°Àº Á¤µµ·Î Á¤È®ÇÏ°Ô ÁõÆøÇÒ ¼ö ÀÖÀ¸¸ç Taq DNA polymerase¿Í µ¿µîÇÑ ÁõÆøÈ¿À²À» °®´Â´Ù. ¶Ç TaKaRa Taq µîÀÇ Taq DNA polymerase¿Í´Â ´Þ¸® Hot Start ¹ýÀ» ÀÌ¿ëÇÏÁö ¾Ê¾Æµµ ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¾ïÁ¦ÇÒ ¼ö ÀÖ´Ù.
È°¼ºÀÇ Á¤ÀÇ
È°¼ºÈ­ salmon sperm DNA¸¦ ÁÖÇü/primer·Î ÀÌ¿ëÇÏ¿© ¾Æ·¡ÀÇ È°¼º ÃøÁ¤ ¹ÝÀÀ¾× Áß¿¡¼­ 74¡É¿¡¼­ 30ºÐ µ¿¾È 10 nmolÀÇ Àüü nucleotide¸¦ »êºÒ¿ë¼º ħÀü¹°·Î ÀüȯÇÏ´Â È°¼ºÀ» 1 U·Î ÇÑ´Ù
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
TaKaRa Taq (5 U/§¡) 50 §¡
20 mM Tris-HCl(pH8.3, 37¡É)
10 mM KCl
6 mM (NH4)2SO4
2 mM MgCl2
0.001% BSA
°¢ 200 ¥ìM dATP¡¤dGTP¡¤dCTP
100 ¥ìM [3H] TTP
³»¿ë
Pyrobest DNA Polymerase 125 U
10¡¿ Pyrobest Buffer
500 §¡
MgCl2 (25 mM)
1 ml
dNTP mixture
400 §¡
Çü»ó
50 mM Tris-HCl(pH8.2)
0.1 mM EDTA
1 mM DTT
0.1% Tween 20
0.1% Nonidet P-40
50% Glycerol
º¸Á¸ -20¡É
÷ºÎ dNTP Mixture*
³óµµ °¢ 2.5 mM
Çü»ó ¼ö¿ë¾×(Na¿°) pH7~9
¼øµµ °¢ 98% ÀÌ»ó
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- 15ºÐ ¿Ï·á! ¿øÁ¶ PCR cloning kit: In-Fusion¢ç Snap Assembly Master Mix
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit: PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword :

High fidelity PCR: PrimeSTAR
>
Long fragment ÁõÆø ¹× GC-rich template ÁõÆø..
Á¤È®ÇÑ GXL DNA Polymerase
>
Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type
GXL Premix ŸÀÔ
>
ÃÖ°í ¼öÁØÀÇ Á¤È®¼ºÀº À¯Áö, °í¼Ó PCR ¼º´É ´ë..
ÃÖ°í Á¤È®µµ-Max v2.
>
ÃÖ°í ·¹º§ÀÇ Á¤È®¼º £¦ ÃÖ°í ¼ÓµµÀÇ ½ÅÀ强
ÃÖ°í Á¤È®µµ-Max DNA Polymerase
>
High Fidelity PCR È¿¼ÒÀÇ ±âÁØ
񃧯-HS DNA Polymerase
>
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
>
GC-rich Template¿¡ °­ÇÑ High Fidelity PCR È¿..
HS with GC Buffer
>
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus