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PCR
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High fidelity PCR: PrimeSTAR
> Pyrobest DNA Polymerase
Pyrobest DNA Polymerase
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Takara
R005A
Pyrobest¢â DNA Polymerase
125 U
132,000¿ø
165,000¿ø
09.02 ~ 10.31
Takara
R005B
Pyrobest¢â DNA Polymerase
500 U (R005A x 4)
596,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
È¿À²ÀûÀÎ PCR ÁõÆøÀ» À§ÇÑ Pyrobest Á¶°Ç¼³Á¤°¡À̵å click!
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº
Pyrococcus sp.
À¯·¡ÀÇ 3¡¯¡æ 5¡¯ exonuclease È°¼º(proof reading È°¼º)À» °®´Â ³»¿¼º ¥áÇü DNA polymeraseÀÌ´Ù.
Pyrococcus furiosus
À¯·¡ÀÇ
Pfu
DNA polymerase³ª
Vent
DNA polymerase¿Í °°Àº Á¤µµ·Î Á¤È®ÇÏ°Ô ÁõÆøÇÒ ¼ö ÀÖÀ¸¸ç Taq DNA polymerase¿Í µ¿µîÇÑ ÁõÆøÈ¿À²À» °®´Â´Ù. ¶Ç TaKaRa Taq µîÀÇ Taq DNA polymerase¿Í´Â ´Þ¸® Hot Start ¹ýÀ» ÀÌ¿ëÇÏÁö ¾Ê¾Æµµ ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¾ïÁ¦ÇÒ ¼ö ÀÖ´Ù.
È°¼ºÀÇ Á¤ÀÇ
È°¼ºÈ salmon sperm DNA¸¦ ÁÖÇü/primer·Î ÀÌ¿ëÇÏ¿© ¾Æ·¡ÀÇ È°¼º ÃøÁ¤ ¹ÝÀÀ¾× Áß¿¡¼ 74¡É¿¡¼ 30ºÐ µ¿¾È 10 nmolÀÇ Àüü nucleotide¸¦ »êºÒ¿ë¼º ħÀü¹°·Î ÀüȯÇÏ´Â È°¼ºÀ» 1 U·Î ÇÑ´Ù
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
TaKaRa Taq (5 U/§¡)
50 §¡
20 mM
Tris-HCl(pH8.3, 37¡É)
10 mM
KCl
6 mM
(NH
4
)
2
SO
4
2 mM
MgCl
2
0.001%
BSA
°¢ 200 ¥ìM
dATP¡¤dGTP¡¤dCTP
100 ¥ìM
[
3
H] TTP
³»¿ë
Pyrobest DNA Polymerase 125 U
10¡¿ Pyrobest Buffer
500 §¡
MgCl
2
(25 mM)
1 ml
dNTP mixture
400 §¡
Çü»ó
50 mM
Tris-HCl(pH8.2)
0.1 mM
EDTA
1 mM
DTT
0.1%
Tween 20
0.1%
Nonidet P-40
50%
Glycerol
º¸Á¸ -20¡É
÷ºÎ dNTP Mixture*
³óµµ
°¢ 2.5 mM
Çü»ó
¼ö¿ë¾×(Na¿°) pH7~9
¼øµµ
°¢ 98% ÀÌ»ó
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit:
Mighty TA-cloning Reagent Set for PrimeSTAR
¢ç
- 15ºÐ ¿Ï·á! ¿øÁ¶ PCR cloning kit:
In-Fusion
¢ç
Snap Assembly Master Mix
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit:
PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose:
SeaPlaque
¢ç
Agarose
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â:
Clontech PCR Thermal Cycler GP
Keyword :
High fidelity PCR: PrimeSTAR
Long fragment ÁõÆø ¹× GC-rich template ÁõÆø..
Á¤È®ÇÑ GXL DNA Polymerase
Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type
GXL Premix ŸÀÔ
ÃÖ°í ¼öÁØÀÇ Á¤È®¼ºÀº À¯Áö, °í¼Ó PCR ¼º´É ´ë..
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í ·¹º§ÀÇ Á¤È®¼º £¦ ÃÖ°í ¼ÓµµÀÇ ½ÅÀ强
ÃÖ°í Á¤È®µµ-Max DNA Polymerase
High Fidelity PCR È¿¼ÒÀÇ ±âÁØ
񃧯-HS DNA Polymerase
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
GC-rich Template¿¡ °ÇÑ High Fidelity PCR È¿..
HS with GC Buffer
Pyrobest DNA Polymerase
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus