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PCR
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DNA PCR °ü·Ã Á¦Ç°
> TaKaRa LA PCR¢â Kit Ver.2.1
TaKaRa LA PCR¢â Kit Ver.2.1
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
RR013A
TaKaRa LA PCR¢â Kit Ver. 2.1
50 ȸ
340,000¿ø
425,000¿ø
09.02 ~ 10.31
Takara
RR013B
TaKaRa LA PCR¢â Kit Ver. 2.1
100 ȸ (RR013A x 2)
808,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Á¦Ç°¼³¸í
TaKaRa LA PCR Kit Ver.2.1Àº LA PCR technologyÀÇ È¿°ú¸¦ ÃÖ´ëÇÑ È°¿ëÇÏ¿© ±× À§·ÂÀ» ¹ßÈÖÇϵµ·Ï °³¹ßÇÑ »õ·Î¿î LA PCR KitÀÌ´Ù. LA PCR¿ëÀ¸·Î ÃÖÀûÈÇÑ Àü¿ë buffer (LA PCR Buffer II)¿Í TaKaRa LA Taq ÀÇ Á¶ÇÕÀ¸·Î, human genomic DNA¸¦ templateÀ¸·Î ±ä ´ÜÆíÀÇ DNA¸¦ ÁõÆøÇÏ´Â µî º¸´Ù ±ä ´ÜÆíÀÇ PCRÀ» °¡´ÉÇÏ°Ô ÇÏ´Â PCR KitÀÌ´Ù. ¹°·Ð ´Ù¾çÇÑ template DNA¿¡ ÀÀ¿ëÇÒ ¼ö ÀÖÀ¸¸ç ªÀº ´ÜÆíÀÇ DNA ÁõÆø¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù. Mg
2+
freeÀÇ buffer¸¦ ÷ºÎÇÏ¹Ç·Î½á ¹ÝÀÀ¾× Á¶¼ºÀ» °ËÅäÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50 §¡ PCR 50 ȸ)
TaKaRa LA Taq (5 U/§¡)
125 U
dNTP Mixture (°¢ 2.5 mM)
400 §¡
10 ¡¿ LA PCR Buffer II (Mg
2+
, plus) (Mg
2+
³óµµ 25 mM)
250 §¡
10 ¡¿ LA PCR Buffer II (Mg
2+
free)
250 §¡
MgCl
2
(25 mM)
500 §¡
Control Template (100 ng/§¡ HT29 À¯·¡ genome DNA)
10 §¡
Control Primer LA3 (10 ¥ìM)
10 §¡
Control Primer LA4 (10 ¥ìM)
10 §¡
¥ë-Hind III digested MW Marker (100 ng/§¡)
20 §¡
2¡¿GC Buffer I (Mg
2+
plus)* (Mg
2+
³óµµ 5 mM)
1.25 §¢
2¡¿GC Buffer II (Mg
2+
plus)* (Mg
2+
³óµµ 5 mM)
1.25 §¢
Control Primer GC1 (10 ¥ìM)
10 §¡
Control Primer GC2 (10 ¥ìM)
10 §¡
* ÁõÆø¿µ¿ªÀÌ GC-rich ¶Ç´Â °ß°íÇÑ 2Â÷ ±¸Á¶¸¦ ÃëÇÏ´Â °ÍÀ¸·Î ÆǴܵǴ °æ¿ì, ¿ì¼± 2¡¿GC Buffer IÀ» »ç¿ëÇÑ´Ù. Buffer IÀ¸·Î ÁõÆøÀÌ ¾î·Á¿î °æ¿ì¿¡´Â 2¡¿GC Buffer II¸¦ »ç¿ëÇÏ´Â °æ¿ì ¹ÝÀÀÀÌ °³¼±µÇ´Â °æ¿ì°¡ ÀÖ´Ù.
º¸Á¸ -20¡É
Control Primer ¼¿
Control Primer LA3:
5¡¯-ACATGATTAGCAAAAGGGCCTAGCTTGGACTCAGA-3¡¯
Control Primer LA4:
5¡¯-TGCACCTGCTCTGTGATTATGACTATCCCACAGTC-3¡¯
* Control TemplateÀÇ 17.5 kb¸¦ ÁõÆøÇÑ´Ù.
Control Primer GC1:
5¡¯-GGGAGGGGACCGGGGAACAGAG-3¡¯
Control Primer GC2:
5¡¯-GAACAGTCCGTCACTTCACGTG-3¡¯
* Control TemplateÀÇ GC-rich ¿µ¿ª 1,255 bp¸¦ ÁõÆøÇÑ´Ù.
Keyword :
DNA PCR °ü·Ã Á¦Ç°
Hot spot ¾øÀÌ RandomÇÏ°Ô µ¹¿¬º¯À̸¦ µµÀÔÇÒ ..
Diversify PCR Random Mutagenesis Kit
LA PCR¢â ¿ë genome DNA Set
TaKaRa LA PCR¢â Kit Ver.2.1
TaKaRa PCR Amplification Kit