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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Subtraction °ü·Ã Á¦Ç° > Clontech PCR-Select Bacterial Genome Subtraction Kit
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Clontech PCR-Select Bacterial Genome Subtraction Kit

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Clontech
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Clontech PCR-Select Bacterial Genome Subtraction Kit
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Clontech PCR-select bacterial genome subtraction Kit¿¡ ÀÇÇØ ¼¼±Õ genomeÀ» È¿À²ÁÁ°Ô ºñ±³ÇÒ ¼ö ÀÖ´Ù. ´Ü ¼ö Àϸ¸¿¡ ÇÑÂÊÀÇ ¼¼±ÕÁÖ¿¡¸¸ Á¸ÀçÇÏ´Â genome ¼­¿­ÀÇ subtractionÀÌ ³¡³­ library¸¦ ¾òÀ» ¼ö ÀÖ´Ù. º» KitÀ» »ç¿ëÇÏ¸é º´¿ø¼º°ú °ü·ÃÇÑ À¯ÀüÀÚ ¿µ¿ªÀÇ µ¿Á¤À̳ª 2 Á¾·ùÀÇ ¼¼±ÕÁÖ °£¿¡¼­ ´Ù¸¥ genome DNAÀÇ µ¿Á¤À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.

PCR-select¿¡¼­´Â 1 ȸÀÇ subtraction hybridization°ú ¼±ÅÃÀû ÁõÆø¿¡ ÀÇÇØ subtractionÀÌ ÀÌ·ç¾îÁö±â ¶§¹®¿¡, ´ÜÀϻ罽 DNAÀÇ ¹°¸®Àû ºÐ¸®°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù. Clontech PCR-select Kit¿¡ ÇÊ¿äÇÑ ¼¼±Õ genome DNA ½Ã·á´Â ´Ü 2 §¶ÀÌ°í ¼Ò¿ä½Ã°£Àº 2~3ÀÏ°£ Á¤µµÀÌ´Ù. º» KitÀº high throughputÇÑ ½Ã·á󸮿¡ ÀûÇÕÇÏ´Ù.

Clontech PCR-select bacterial genome subtraction Kit¿¡´Â 7 ¹ÝÀÀºÐÀÇ ½Ã¾à°ú user manualÀÌ Æ÷ÇԵǾî ÀÖ´Ù. Àå°Å¸®(LD) PCR¿ë PCR È¥ÇÕÈ¿¼Ò´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. Advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.


±×¸² 1. À¯¿¬°ü°è°¡ ¾ø´Â H. pyloriÁÖ °£¿¡¼­ÀÇ À¯ÀüÀÚ ÇÔ·® Â÷ÀÌ °ü°è°¡ ¾ø´Â 2 ÁÖÀÇ H. pylori¸¦ »ç¿ëÇÏ¿© PCR-select¿¡ ÀÇÇÑ subtractionÀ» ½Ç½ÃÇÏ¿´´Ù. Tester¿¡ J166 ÁÖ¸¦ »ç¿ëÇÏ°í, driver¿¡´Â 26,695 ÁÖ¸¦ »ç¿ëÇÏ¿´´Ù. PCR ÁõÆøÀ» ½Ç½ÃÇÑ ÈÄ¿¡ 1.5 % agarose gelÀ» »ç¿ëÇÏ¿© subtract ÀüÀÇ tester(lane 1) ¹× driver(lane 2)ÀÇ Àü±â¿µµ¿À» ½Ç½ÃÇÏ°í(0.3 mg/lane) nylon filter¿¡ Àü»çÇÏ¿´´Ù. SubtractionÀÌ ³¡³­ library À¯·¡ÀÇ cloneÀ» ¹«ÀÛÀ§·Î ¼±ÅÃÇÏ¿© filter¿Í hybridized½ÃÄ×´Ù(Panel A-D) 20 cloneÀÇ Çؼ®À» ½Ç½ÃÇÑ °á°ú, tester¸¸À¸·Î hybridized½ÃŲ clone(¿¹, Panel A, B) ¶Ç´Â tester ÃøÀÌ ±³¹è È¿À²ÀÌ ³ô¾Ò´ø clone(¿¹, Panel C)Àº 10 cloneÀÌ ÀÖ¾ú´Ù.
±¸¼º¹°
  • Rsa I
  • Rsa I restriction buffer
  • T4 DNA ligase
  • Adaptors 1 & 2R
  • DNA ligation buffer
  • Dilution buffer
  • Hybridization buffer
  • PCR primer 1
  • Nested primers 1 & 2R
  • PCR control subtracted genomic DNA
  • E. coli strain B genomic DNA
  • Control DNA
  • 23S RNA forward & reverse primers
  • User manual(PT3170-1)
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½Ç¿Â : Hybridization buffer
-20 ¡É : ÀÌ¿ÜÀÇ ¸ðµç ½Ã¾à
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  • ¼¼±Õ genome subtractive hybridization
  • À¯ÀüÀÚ Æ¯Á¤
Âü°í¹®Çå
1. Diatchenko, L., et al.(1996) Proc. Natl. Acad. Sci. USA 93:6025-6030.
2. Gurskaya, N. G., et al.(1996) Anal. Biochem. 240:90-97.
3. PCR-select cDNA subtraction Kit(October 1995) Clontechniques X(4):2-5.
4. Akopyants, N. S., et al.(1998) Proc. Natl. Acad. Sci. USA 95:13108-13113.

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PCR Subtraction °ü·Ã Á¦Ç°
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