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PCR Subtraction °ü·Ã Á¦Ç°
> Clontech PCR-Select Bacterial Genome Subtraction Kit
¼¼±Õ GenomeÀ» È¿À²ÀûÀ¸·Î ºñ±³ÇÒ ¼ö ÀÖ´Â Kit
Clontech PCR-Select Bacterial Genome Subtraction Kit
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Clontech
637404
Clontech PCR-Select Bacterial Genome Subtraction Kit
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2,226,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
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񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
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SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
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Premix ½Ã¾à (Hot Start..
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Direct PCR: MightyAmp
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Colony PCR¿ë premix
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Fast PCR¿ë premix
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EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
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BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
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Advantage GC 2 Polymer..
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Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
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TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
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RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
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TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
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TITANIUM DNA AMPLIFICA..
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O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
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TaKaRa źÀú±Õ PCR Dete..
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Welchii±Õ °ËÃâ¿ë Prime..
Clontech PCR-select bacterial genome subtraction Kit¿¡ ÀÇÇØ ¼¼±Õ genomeÀ» È¿À²ÁÁ°Ô ºñ±³ÇÒ ¼ö ÀÖ´Ù. ´Ü ¼ö Àϸ¸¿¡ ÇÑÂÊÀÇ ¼¼±ÕÁÖ¿¡¸¸ Á¸ÀçÇÏ´Â genome ¼¿ÀÇ subtractionÀÌ ³¡³ library¸¦ ¾òÀ» ¼ö ÀÖ´Ù. º» KitÀ» »ç¿ëÇÏ¸é º´¿ø¼º°ú °ü·ÃÇÑ À¯ÀüÀÚ ¿µ¿ªÀÇ µ¿Á¤À̳ª 2 Á¾·ùÀÇ ¼¼±ÕÁÖ °£¿¡¼ ´Ù¸¥ genome DNAÀÇ µ¿Á¤À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
PCR-select¿¡¼´Â 1 ȸÀÇ subtraction hybridization°ú ¼±ÅÃÀû ÁõÆø¿¡ ÀÇÇØ subtractionÀÌ ÀÌ·ç¾îÁö±â ¶§¹®¿¡, ´ÜÀϻ罽 DNAÀÇ ¹°¸®Àû ºÐ¸®°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù. Clontech PCR-select Kit¿¡ ÇÊ¿äÇÑ ¼¼±Õ genome DNA ½Ã·á´Â ´Ü 2 §¶ÀÌ°í ¼Ò¿ä½Ã°£Àº 2~3ÀÏ°£ Á¤µµÀÌ´Ù. º» KitÀº high throughputÇÑ ½Ã·á󸮿¡ ÀûÇÕÇÏ´Ù.
Clontech PCR-select bacterial genome subtraction Kit¿¡´Â 7 ¹ÝÀÀºÐÀÇ ½Ã¾à°ú user manualÀÌ Æ÷ÇԵǾî ÀÖ´Ù. Àå°Å¸®(LD) PCR¿ë PCR È¥ÇÕÈ¿¼Ò´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. Advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
±×¸² 1. À¯¿¬°ü°è°¡ ¾ø´Â H. pyloriÁÖ °£¿¡¼ÀÇ À¯ÀüÀÚ ÇÔ·® Â÷ÀÌ °ü°è°¡ ¾ø´Â 2 ÁÖÀÇ H. pylori¸¦ »ç¿ëÇÏ¿© PCR-select¿¡ ÀÇÇÑ subtractionÀ» ½Ç½ÃÇÏ¿´´Ù. Tester¿¡ J166 ÁÖ¸¦ »ç¿ëÇÏ°í, driver¿¡´Â 26,695 ÁÖ¸¦ »ç¿ëÇÏ¿´´Ù. PCR ÁõÆøÀ» ½Ç½ÃÇÑ ÈÄ¿¡ 1.5 % agarose gelÀ» »ç¿ëÇÏ¿© subtract ÀüÀÇ tester(lane 1) ¹× driver(lane 2)ÀÇ Àü±â¿µµ¿À» ½Ç½ÃÇÏ°í(0.3 mg/lane) nylon filter¿¡ Àü»çÇÏ¿´´Ù. SubtractionÀÌ ³¡³ library À¯·¡ÀÇ cloneÀ» ¹«ÀÛÀ§·Î ¼±ÅÃÇÏ¿© filter¿Í hybridized½ÃÄ×´Ù(Panel A-D) 20 cloneÀÇ Çؼ®À» ½Ç½ÃÇÑ °á°ú, tester¸¸À¸·Î hybridized½ÃŲ clone(¿¹, Panel A, B) ¶Ç´Â tester ÃøÀÌ ±³¹è È¿À²ÀÌ ³ô¾Ò´ø clone(¿¹, Panel C)Àº 10 cloneÀÌ ÀÖ¾ú´Ù.
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Rsa I
Rsa I restriction buffer
T4 DNA ligase
Adaptors 1 & 2R
DNA ligation buffer
Dilution buffer
Hybridization buffer
PCR primer 1
Nested primers 1 & 2R
PCR control subtracted genomic DNA
E. coli strain B genomic DNA
Control DNA
23S RNA forward & reverse primers
User manual(PT3170-1)
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½Ç¿Â : Hybridization buffer
-20 ¡É : ÀÌ¿ÜÀÇ ¸ðµç ½Ã¾à
Àû¿ëºÐ¾ß
¼¼±Õ genome subtractive hybridization
À¯ÀüÀÚ Æ¯Á¤
Âü°í¹®Çå
1. Diatchenko, L., et al.(1996) Proc. Natl. Acad. Sci. USA 93:6025-6030.
2. Gurskaya, N. G., et al.(1996) Anal. Biochem. 240:90-97.
3. PCR-select cDNA subtraction Kit(October 1995) Clontechniques X(4):2-5.
4. Akopyants, N. S., et al.(1998) Proc. Natl. Acad. Sci. USA 95:13108-13113.
Keyword :
PCR Subtraction °ü·Ã Á¦Ç°
¼¼±Õ GenomeÀ» È¿À²ÀûÀ¸·Î ºñ±³ÇÒ ¼ö ÀÖ´Â Kit
Clontech PCR-Select Bacterial Genome Subtraction Kit
½Å±Ô À¯ÀüÀÚ ¶Ç´Â ¹ßÇö·®¿¡ Â÷ÀÌ°¡ ÀÖ´Â À¯ÀüÀÚ..
Clontech PCR-Select cDNA Subtraction Kit
Substraction ÀüÈÄ cDNA ¹ßÇö Â÷À̸¦ °£´ÜÇÏ°Ô ..
Clontech PCR-Select Differencial Screening Kit