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PCR
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PCR Enzyme (Clontech)
> Advantage HD Polymerase Mix
Super accuracy, high specificity
Advantage HD Polymerase Mix
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Clontech
639241
Advantage HD Polymerase Mix
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ÃÖ»óÀÇ Á¤È®¼º°ú ¶Ù¾î³ ÁõÆø È¿À²ÀÇ ClontechÀÇ DNA polymerase !!
Advantage HD DNA Polymerase
- ÃÖ»óÀÇ fidelity¿Í ȸ¼ö·®
- pfu °è¿ DNA polymeraseº¸´Ù ¶Ù¾î³ ÁõÆø È¿À²·Î Á¶°Ç ¼³Á¤ÀÌ °£Æí
- cDNA cloning, site-directed mutagenesis, ±×¸®°í mutation genotyping (SNPºÐ¼®)¿¡ ÃÖÀû
Superb Accuracy & Efficiency
±Øµµ·Î ³·Àº error rate·Î 250,000 bp ´ç ´Ü, 12°³ÀÇ error¸¸ÀÌ È®ÀÎ µÇ¾ú´Ù. Taq DNA Polymeraseº¸´Ù 10¹èÁ¤µµ ³ôÀº fidelity ÀÌ´Ù.
ªÀº ¹ÝÀÀ ½Ã°£
Advantage HD DNA polymerase´Â ¸Å¿ì ³ôÀº priming È¿À²·Î ´õ ªÀº annealing ½Ã°£À¸·Îµµ ´õ ³ôÀº specificity¿Í sensitity¸¦ º¸¿´´Ù.
¶ÇÇÑ Anti-taq antibody¿¡ ÀÇÇØ Ãß°¡ÀûÀÎ 7~10ºÐÁ¤µµÀÇ predanature ½Ã°£ÀÌ ÇÊ¿ä ¾ø±â ¶§¹®¿¡ ´õ¿í È¿À²ÀûÀÌ°í ½Å¼ÓÇÏ°Ô ¹ÝÀÀÀ» À̲ø ¼ö ÀÖ´Ù. º» Á¦Ç° ÀÌ¿ë½Ã¿£ ±âÁ¸ Taqº¸´Ù annealing timeÀ» ª°Ô ÀâÀ¸¼¼¿ä(¸Þ´º¾óÀ» ÇÊÈ÷ ¼÷ÁöÇϼ¼¿ä).
Robust Amplification
½ÇÇè °á°ú human genomic DNA´Â 8.5kb ±îÁö, E. coli genomic DNA´Â 10kb±îÁö, ±×¸®°í ¥ë DNA´Â ÃÖ´ë 22 kb±îÁö ÁõÆøÇÒ ¼ö ÀÖ´Ù.
GC richÀÎ Thermus thermophilus HB8 genomic DNA¸¦ ÁÖÇüÀ¸·Î¼ ÀÓÀÇ¿¡ ¼±ÅÃÇÑ 8 ¿µ¿ª(ÁõÆø »çÀÌÁî´Â °¢°¢ ¾à 500 bp)À» PCR ÁõÆø ÈÄ, º¤ÅÍ¿¡ Ŭ·Î´× ÇØ, °¢ ¹è¿¿¡ ´ëÇØ º¹¼ö Ŭ·ÐÀ» ¼±ÅÃÇÏ¿© ±× ¼¿À» È®ÀÎÇØ, mutation frequency¸¦ È®ÀÎÇÏ¿´´Ù.
±× °á°ú, Advantage HD DNA Polymerase´Â Taq DNA Polymerase¿Í ºñ±³Çؼ 10¹è fidelity°¡ ³ô¾ÒÀ¸¸ç ±âÁ¸ pfu°è¿º¸´Ù ¶Ù¾î³ ÁõÆø È¿À²·Î Á¶°Ç ¼³Á¤ÀÌ °£ÆíÇÏ´Ù.
Figure 1. ¶Ù¾î³ fidelity È¿¼Ò- Advantage HD DNA Polymerase. The error rate (mutation frequency) for Advantage HD Polymerase is lower than that of a number of commonly used DNA polymerases. Eight arbitrarily selected GC-rich regions from Thermus thermophilus HB8 genomic DNA were amplified with Advantage HD Polymerase and other polymerases. PCR products (approximately 500 bp representing each region) were cloned into suitable plasmids. Multiple clones were selected and subjected to sequence analysis to determine the error rate percentage (e.g., 1 error/100,000 bp = 0.001%)
Figure 2. ´ÜÀÏ PCR cycling Á¶°ÇÀ¸·Î Advantage HD Polymerase ÁõÆø. 1-3 ¥ìl PCR products were run on a 1% agarose/EtBr gel. Lanes 2-7: Different sized fragments of the bovine pancreatic trypsin inhibitor (BPTI) gene were amplified from 100 ng of calf thymus genomic DNA. Lane 2: 500 bp. Lane 3: 900 bp. Lane 4: 1,500 bp. Lane 5: 2,000 bp. Lane 6: 2,500 bp. Lane 7: 3,500 bp. Lane 8: Amplification of 414 bp c-jun fragment from 100 ng human genomic DNA. Lane 9: Amplification of a ~3,000 bp ¥ë fragment from 1 ng ¥ëgt10 lysate. Lane 1: Mixture (1:1) of ¥ë-Hind III and ¥ÕX174-Hae III digests.
Àû¿ëºÐ¾ß
- cDNA cloning
- site-directed mutagenesis
- mutation genotyping (i.e., SNP analysis)
Keyword :
PCR Enzyme (Clontech)
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³ôÀº fidelity¿Í ÁõÆøÈ¿À²À» °âºñÇÑ µ¿°á°ÇÁ¶µÈ..
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³ôÀº ÁõÆø È¿À²ÀÇ µ¿°á°ÇÁ¶µÈ PCR mastermix
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