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Home > ÀüÁ¦Ç°º¸±â > PCR > Standard PCR: Ex, LA, rTaq > Ex Taq Hot Start Version
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö

Ex Taq Hot Start Version

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR006A
TaKaRa Ex Taq¢ç Hot Start Version
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
250 U
156,800¿ø 
196,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR006A_DS.v2110Da.pdf
Takara
RR006B
TaKaRa Ex Taq¢ç Hot Start Version
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (RR006A x 4)
708,000¿ø 

Á¦Ç°¼³¸í
TaKaRa Ex Taq¢ç Àº TaKaRa Taq¢âÀÇ ÀÔÁõµÈ ¼º´É°ú È¿À²ÀûÀÎ 3¡¯ a 5¡¯ exonucleaseÀÇ ±³Á¤´É·ÂÀ» ¸ðµÎ °®Ãá °íÈ¿À², °í°¨µµÀÇ DNA polymeraseÀÌ´Ù.
º» Á¦Ç°Àº TaKaRa Ex Taq¢ç¿¡ Anti-Taq antibody¸¦ ÀÌ¿ëÇÑ Hot Start PCR¿ë È¿¼ÒÀ̸ç, °í¿Â¿¡ µµ´ÞÇÒ ¶§±îÁö´Â antibody°¡ °áÇÕÇÏ°í ÀÖ¾î polymerase È°¼ºÀ» ¾ïÁ¦ÇÑ´Ù. µû¶ó¼­ PCR cycleÀ» ½ÃÀÛÇϱâ Àü¿¡ ¹ß»ýÇÒ ¼ö ÀÖ´Â misprimingÀ̳ª primer dimer¿¡ ÀÇÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¹æÁöÇÒ ¼ö ÀÖ´Ù.
Anti-Taq antibody´Â PCR cycleÀÇ ÃÖÃÊ DNA denaturation ´Ü°è¿¡¼­ º¯¼ºµÇ¹Ç·Î Anti-Taq antibodyÁ¦°Å¸¦ À§ÇÑ Ãß°¡ °úÁ¤Àº ÇÊ¿äÇÏÁö ¾Ê°í, ±âÁ¸¿¡ »ç¿ëÇÏ´ø PCR Á¶°ÇÀ» ±×´ë·Î »ç¿ëÇÑ´Ù.
³»¿ë (250 U)
TaKaRa Ex Taq Hot Start Version (TaKaRa Code RR006A)
1. TaKaRa Ex Taq HS (5 U/§¡)
50 §¡
2. 10¡¿ Ex Taq Buffer (20 mM Mg2+ plus)
1 §¢
3. dNTP Mixture (°¢ 2.5 mM)
800 §¡
º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl (pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

NP-40

50%

Glycerol

÷ºÎ dNTP Mixture Á¶¼º*

³óµµ

°¢ 2.5 mM

Çü»ó

¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9

¼øµµ

°¢ 98% ÀÌ»ó

*dATP, dCTP, dGTP, dTTPµî ¸ô È¥ÇÕ¹°·Î Èñ¼®ÇÏÁö ¾Ê°í ±×´ë·Î PCR¹ÝÀÀ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇØ
TaKaRa Ex Taq¢ç HS¸¦ ÀÌ¿ëÇÏ¿© ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3¡¯ ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector pMD20 (Code 3270) ¶Ç´Â T-vector pMD19 (Simple) (Code 3271)¿¡ cloningÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸»´Ü ÆòÈ°È­ ¹× ÀλêÈ­ ÈÄ, ÆòÈ° ¸»´Ü vector¿¡ cloningÇÒ ¼öµµ ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- Premix ŸÀÔÀÇ Hot start ¹öÀü: Premix Ex Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
- 2X PCR master mix: Premix Taq¢â (Ex Taq Version 2.0) (500 ¥ìl¡¿6 tube)
- ¿ªÀü»ç¹ÝÀÀÀ» À§ÇÑ all-in-One kit: PrimeScript¢â 1st strand cDNA Synthesis Kit
- 5x premix ¿ªÀü»ç ½Ã¾à: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword : hot start PCR

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