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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Subtraction °ü·Ã Á¦Ç° > Clontech PCR-Select cDNA Subtraction Kit
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Clontech PCR-Select cDNA Subtraction Kit

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Clontech
637401
Clontech PCR-Select cDNA Subtraction Kit
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- ÇÊ¿äÇÑ poly A+ RNA´Â ´Ü 2 §¶
- cDNA subtraction °úÁ¤ÀÌ ´Ü 3~4 Àϸ¸¿¡ ¿Ï·á
Clontech PCR-select cDNA subtraction KitÀ» »ç¿ëÇÏ¸é ¹ßÇö·®¿¡ Â÷ÀÌ°¡ Àִ ƯÀÌÀû À¯ÀüÀÚ(2 Á¾·ùÀÇ mRNAÁý´Ü ÇÑ Ãø¿¡¼­ ¹ßÇöÇÏ°í ÀÖÁö¸¸ ´Ù¸¥ Ãø¿¡¼­´Â °¨¼Ò ¶Ç´Â ¹ßÇöÇÏÁö ¾Ê´Â À¯ÀüÀÚ ; 1~4)ÀÇ ¼±ÅÃÀû ÁõÆøÀ» È¿À²ÀûÀ¸·Î ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. ÀϹÝÀûÀ¸·Î Èñ¼ÒÇÑ Àü»ç »ý¼º¹°ÀÇ ÃëµæÀº »ó´çÈ÷ °ï¶õÇÏÁö¸¸, º» ¹æ¹ýÀº ÀÌ·¯ÇÑ cDNA µ¿Á¤¿¡ ƯÈ÷ ÀûÇÕÇÏ´Ù. Clontech PCR-select¹ýÀº single strand cDNA¿Í double strand cDNAÀÇ ¹°¸®Àû ºÐ¸®¸¦ ÇÊ¿ä·Î ÇÏ´Â ´Ù¸¥ ¹æ¹ý°ú´Â ´Þ¸®, ¸ñÀû ¼­¿­¸¸À» Áö¼öÀûÀ¸·Î ÁõÆøÇÒ ¼ö ÀÖ´Ù. º» ¹æ¹ýÀº ´ÙÀ½°ú °°Àº ´Ù¾çÇÑ ÀåÁ¡À» °¡Áö°í ÀÖ´Ù. - Á¶ÀÛ´Ü°è°¡ Àû°í Á÷Á¢ÀûÀÎ ¹æ¹ý : PCR-select¹ý¿¡¼­´Â subtraction hybridization ¹× ƯÀÌÀû ¹ßÇö À¯ÀüÀÚ ÁõÆøÀÇ ÀÏ·ÃÀÇ Á¶ÀÛ °úÁ¤¿¡¼­ À¯ÀüÀÚ subtractionÀÌ ÀÌ·ç¾îÁø´Ù. ¹°¸®Àû ºÐ¸®´Â ÇÊ¿äÇÏÁö ¾Ê´Ù. - Èñ¼ÒÇÑ Àü»ç »ý¼º¹°À» 1,000 ¹è ÀÌ»óÀ¸·Î ³óÃà : PCR-select¿¡¼­´Â Àü»ç »ý¼º¹°ÀÇ Á¸ÀçÀ² º¸Á¤°ú subtraction Á¶ÀÛÀ» µ¿½Ã¿¡ ÇÑ´Ù. ±× °á°ú, ƯÀÌÀûÀ¸·Î ¹ßÇöµÈ Èñ¼ÒÇÑ Àü»ç »ý¼º¹°À» ¾òÀ» È®·üÀÌ »ó´çÈ÷ ³ô¾ÆÁø´Ù. - ´Ü 2 §¶ÀÇ poly A+ RNA·Î subtractionÀÌ °¡´É : PCR-select¿¡¼­ ÇÊ¿äÇÑ poly A+ RNA·®Àº ´Ü 2 §¶À̹ǷÎ, Á¶Á¦°¡ °ï¶õÇÑ ¼Ò·®ÀÇ RNA ½Ã·á¿¡´Â ƯÈ÷ À¯¿ëÇÏ´Ù. total RNA°¡ ng Á¤µµ¹Û¿¡ ¾ø´Â °æ¿ì´Â super SMART PCR cDNA synthesis KitÀ» »ç¿ëÇÏ¿© clontech PCR-select cDNA subtraction¿¡ »ç¿ëÇÏ´Â °íÇ°ÁúÀÇ cDNA¸¦ Á¦ÀÛÇØ¾ß ÇÑ´Ù.



±×¸²1. Southern blot ºÐ¼®

Clontech PCR-select cDNA subtraction Kit¿¡´Â cDNA ÇÕ¼º 7 ȸºÐ ½Ã¾à°ú user manualÀÌ Ã·ºÎµÇ¾î ÀÖ´Ù. LD(Long Distance) PCR¿ë PCR È¿¼Ò mix´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. Clontech¿¡¼­´Â advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
±¸¼º¹°
  • AMV reverse transcriptase
  • cDNA synthesis primer
  • First-strand buffer
  • Second-strand enzyme cocktail
  • Second-strand buffer
  • T4 DNA polymerase
  • Rsa I
  • Rsa I restriction buffer
  • T4 DNA ligase
  • Adaptors 1 & 2R
  • DNA ligation buffer
  • Dilution buffer
  • Hybridization buffer
  • PCR primer 1
  • Nested PCR primers 1 & 2R
  • PCR control subtracted cDNA
  • Control poly A+ RNA
  • Control DNA
  • G3PDH 5' & 3' primers
  • dNTP mix
  • EDTA/glycogen mix
  • Ammonium acetate
  • Sterile H2O
  • User manual(PT1117-1)
º¸Á¸
-70 ¡É : Control poly A+ RNA
½Ç¿Â : hybridization buffer
-20 ¡É : ÀÌ¿ÜÀÇ ¸ðµç ½Ã¾à
Àû¿ëºÐ¾ß
  • Subtractive hybridization
  • À¯ÀüÀÚ Æ¯Á¤
  • ƯÀÌÀû À¯ÀüÀÚ ¹ßÇöÇؼ®
  • À¯ÀüÀÚ ¹ßÇöÇؼ®
  • cDNA subtraction
Âü°í¹®Çå
1. Diatchenko, L., et al.(1996) Proc.Natl.Acad.Sci. USA 93:6025-6030.
2. Gurskaya, N. G., et al.(1996) Anal. Biochem. 240:90-97.
3. PCR-select cDNA subtraction Kit(October 1995) Clontechniques X(4):2-5.
4. Diatchenko, L., et al.(1998) In RT-PCR methods for gene cloning and analysis, Eds. Siebert, P. D., et al.(BioTechniques books, MA), pp.213-239.

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PCR Subtraction °ü·Ã Á¦Ç°
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