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PCR Subtraction °ü·Ã Á¦Ç°
> Clontech PCR-Select cDNA Subtraction Kit
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Clontech PCR-Select cDNA Subtraction Kit
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Clontech
637401
Clontech PCR-Select cDNA Subtraction Kit
7 ȸ
(Package)
2,226,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
½Å±Ô À¯ÀüÀÚ, ¹ßÇö·®¿¡ Â÷ÀÌ°¡ ÀÖ´Â À¯ÀüÀÚ ºÐ¸®¿¡ ÀÌ»óÀû
- Èñ¼ÒÇÑ Àü»ç »ý¼º¹°À» 1,000 ¹è ÀÌ»óÀ¸·Î ³óÃà
- ÇÊ¿äÇÑ poly A+ RNA´Â ´Ü 2 §¶
- cDNA subtraction °úÁ¤ÀÌ ´Ü 3~4 Àϸ¸¿¡ ¿Ï·á
Clontech PCR-select cDNA subtraction KitÀ» »ç¿ëÇÏ¸é ¹ßÇö·®¿¡ Â÷ÀÌ°¡ Àִ ƯÀÌÀû À¯ÀüÀÚ(2 Á¾·ùÀÇ mRNAÁý´Ü ÇÑ Ãø¿¡¼ ¹ßÇöÇÏ°í ÀÖÁö¸¸ ´Ù¸¥ Ãø¿¡¼´Â °¨¼Ò ¶Ç´Â ¹ßÇöÇÏÁö ¾Ê´Â À¯ÀüÀÚ ; 1~4)ÀÇ ¼±ÅÃÀû ÁõÆøÀ» È¿À²ÀûÀ¸·Î ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. ÀϹÝÀûÀ¸·Î Èñ¼ÒÇÑ Àü»ç »ý¼º¹°ÀÇ ÃëµæÀº »ó´çÈ÷ °ï¶õÇÏÁö¸¸, º» ¹æ¹ýÀº ÀÌ·¯ÇÑ cDNA µ¿Á¤¿¡ ƯÈ÷ ÀûÇÕÇÏ´Ù. Clontech PCR-select¹ýÀº single strand cDNA¿Í double strand cDNAÀÇ ¹°¸®Àû ºÐ¸®¸¦ ÇÊ¿ä·Î ÇÏ´Â ´Ù¸¥ ¹æ¹ý°ú´Â ´Þ¸®, ¸ñÀû ¼¿¸¸À» Áö¼öÀûÀ¸·Î ÁõÆøÇÒ ¼ö ÀÖ´Ù. º» ¹æ¹ýÀº ´ÙÀ½°ú °°Àº ´Ù¾çÇÑ ÀåÁ¡À» °¡Áö°í ÀÖ´Ù.
- Á¶ÀÛ´Ü°è°¡ Àû°í Á÷Á¢ÀûÀÎ ¹æ¹ý
: PCR-select¹ý¿¡¼´Â subtraction hybridization ¹× ƯÀÌÀû ¹ßÇö À¯ÀüÀÚ ÁõÆøÀÇ ÀÏ·ÃÀÇ Á¶ÀÛ °úÁ¤¿¡¼ À¯ÀüÀÚ subtractionÀÌ ÀÌ·ç¾îÁø´Ù. ¹°¸®Àû ºÐ¸®´Â ÇÊ¿äÇÏÁö ¾Ê´Ù.
- Èñ¼ÒÇÑ Àü»ç »ý¼º¹°À» 1,000 ¹è ÀÌ»óÀ¸·Î ³óÃà
: PCR-select¿¡¼´Â Àü»ç »ý¼º¹°ÀÇ Á¸ÀçÀ² º¸Á¤°ú subtraction Á¶ÀÛÀ» µ¿½Ã¿¡ ÇÑ´Ù. ±× °á°ú, ƯÀÌÀûÀ¸·Î ¹ßÇöµÈ Èñ¼ÒÇÑ Àü»ç »ý¼º¹°À» ¾òÀ» È®·üÀÌ »ó´çÈ÷ ³ô¾ÆÁø´Ù.
- ´Ü 2 §¶ÀÇ poly A+ RNA·Î subtractionÀÌ °¡´É
: PCR-select¿¡¼ ÇÊ¿äÇÑ poly A+ RNA·®Àº ´Ü 2 §¶À̹ǷÎ, Á¶Á¦°¡ °ï¶õÇÑ ¼Ò·®ÀÇ RNA ½Ã·á¿¡´Â ƯÈ÷ À¯¿ëÇÏ´Ù. total RNA°¡ ng Á¤µµ¹Û¿¡ ¾ø´Â °æ¿ì´Â super SMART PCR cDNA synthesis KitÀ» »ç¿ëÇÏ¿© clontech PCR-select cDNA subtraction¿¡ »ç¿ëÇÏ´Â °íÇ°ÁúÀÇ cDNA¸¦ Á¦ÀÛÇØ¾ß ÇÑ´Ù.
±×¸²1. Southern blot ºÐ¼®
Clontech PCR-select cDNA subtraction Kit¿¡´Â cDNA ÇÕ¼º 7 ȸºÐ ½Ã¾à°ú user manualÀÌ Ã·ºÎµÇ¾î ÀÖ´Ù. LD(Long Distance) PCR¿ë PCR È¿¼Ò mix´Â º°µµ·Î ÁغñÇØ¾ß ÇÑ´Ù. Clontech¿¡¼´Â advantage 2 polymerase mix(Cat. No. 639201) »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
±¸¼º¹°
AMV reverse transcriptase
cDNA synthesis primer
First-strand buffer
Second-strand enzyme cocktail
Second-strand buffer
T4 DNA polymerase
Rsa I
Rsa I restriction buffer
T4 DNA ligase
Adaptors 1 & 2R
DNA ligation buffer
Dilution buffer
Hybridization buffer
PCR primer 1
Nested PCR primers 1 & 2R
PCR control subtracted cDNA
Control poly A+ RNA
Control DNA
G3PDH 5' & 3' primers
dNTP mix
EDTA/glycogen mix
Ammonium acetate
Sterile H2O
User manual(PT1117-1)
º¸Á¸
-70 ¡É : Control poly A+ RNA
½Ç¿Â : hybridization buffer
-20 ¡É : ÀÌ¿ÜÀÇ ¸ðµç ½Ã¾à
Àû¿ëºÐ¾ß
Subtractive hybridization
À¯ÀüÀÚ Æ¯Á¤
ƯÀÌÀû À¯ÀüÀÚ ¹ßÇöÇؼ®
À¯ÀüÀÚ ¹ßÇöÇؼ®
cDNA subtraction
Âü°í¹®Çå
1. Diatchenko, L., et al.(1996) Proc.Natl.Acad.Sci. USA 93:6025-6030.
2. Gurskaya, N. G., et al.(1996) Anal. Biochem. 240:90-97.
3. PCR-select cDNA subtraction Kit(October 1995) Clontechniques X(4):2-5.
4. Diatchenko, L., et al.(1998) In RT-PCR methods for gene cloning and analysis, Eds. Siebert, P. D., et al.(BioTechniques books, MA), pp.213-239.
Keyword :
PCR Subtraction °ü·Ã Á¦Ç°
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