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Home > ÀüÁ¦Ç°º¸±â > PCR > Standard PCR: Ex, LA, rTaq > Premix ½Ã¾à (Ex, LA Taq)
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Premix ½Ã¾à (Ex, LA Taq)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R004A
Premix Taq¢â (TaKaRa Taq¢â Version 2.0) (500 ¥ìl¡¿6 tube)
°ü·ÃÇмú ±¸¸ÅÇϱâ
120 ȸ
(50 ¥ìl PCR)
97,600¿ø 
122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R004A_DS.v2110Da.pdf
Takara
RR003A
Premix Taq¢â (Ex Taq Version 2.0) (500 ¥ìl¡¿6 tube)
°ü·ÃÇмú ±¸¸ÅÇϱâ
120 ȸ
(50 ¥ìl PCR)
121,600¿ø 
152,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR003A_DS.v2110Da.pdf
Takara
RR004
One Shot LA PCR¢â Mix Ver. 2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìl ¡¿ 24 tube
97,600¿ø 
122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR004_DS.v2110Da.pdf

Á¦Ç° ¼³¸í
º» ½Ã¸®Áî´Â PCR¿ë DNA polymerase, PCR¿ë ¹ÝÀÀ buffer, dNTP Mixture¸¦ È¥ÇÕÇÑ 2x premix Á¦Ç°À¸·Î, PCR ¹ÝÀÀ½Ã buffer, dNTP MixtureµîÀ» µû·Îµû·Î ÷°¡ÇÒ ÇÊ¿ä°¡ ¾ø¾î Á¶ÀÛÀÌ °£ÆíÇÏ´Ù.

- Premix Taq¢â (TaKaRa Taq¢â Version), Premix Taq¢â (TaKaRa Ex Taq¢â 2.0) : 2 x premix solution (°¢ 500 §¡ x 6 vials)
- One Shot LA PCR Mix Ver. 2.0 : 0.2 §¢ PCR tube¿¡ 25 §¡¾¿ ºÐÁֵǾî ÀÖ´Â premix
º¸Á¸ -20 ¡É (4¡É¿¡¼­ 3°³¿ù º¸Á¸ °¡´É)
Á¶¼º

Premix Taq¢â (TaKaRa Taq¢â Version 2.0) (Code R004A)

TaKaRa Taq

1.25 U/25 §¡

dNTP Mixture

2¡¿conc. ; °¢ 0.4 mM

PCR buffer

2¡¿conc. ; 20 mM Tris-HCl (Ph 8.9), 100 mM KCl, 3 mM MgCl2

 

Premix Taq¢â (Ex Taq¢â Version 2.0) (Code RR003A)

TaKaRa Ex Taq

1.25 U/25 §¡

dNTP Mixture

2¡¿conc. ; °¢ 0.4 mM

Ex Taq buffer

2¡¿conc. ; 4 mM Mg2+Æ÷ÇÔ

 

 

One Shot LA PCR¢â Mix Ver. 2.0 (Code RR004)

TaKaRa LA Taq

2.5 U/25 §¡

dNTP Mixture

2¡¿conc. ; °¢ 0.8 mM

LA PCR Buffer II

2¡¿conc. ; 5 mM Mg2+ Æ÷ÇÔ2


* 2.0 ¹öÀüÀº ½Ã¾à Á¶¼ºÀ» °³·®ÇÏ¿© ¹ÝÀÀ¼ºÀÌ ³ô¾ÆÁ³½À´Ï´Ù.
PCR ÁõÆø»çÀÌÁî
- ¥ë DNA¸¦ ÁÖÇüÀ¸·Î »ç¿ëÇÑ °æ¿ì:
Premix Taq (TaKaRa Taq Version 2.0): 8 kb
Premix Taq (Ex Taq Version 2.0): 20 kb
One Shot LA PCR Mix Ver. 2.0: 28 kb

- Human genomic DNA¸¦ ÁÖÇüÀ¸·Î »ç¿ëÇÑ °æ¿ì:
Premix Taq (TaKaRa TaqVersion 2.0): ¾à 3 kb
Premix Taq (Ex TaqVersion 2.0): ¾à 3 kb
One Shot LA PCR Mix Ver. 2.0: 17.5 kb
ÁõÆø »ê¹°ÀÇ ¸»´Ü
º» Á¦Ç°À» ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3£§¸»´Ü¿¡ A°¡ 1 ¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ PCR »ê¹°À» Á÷Á¢ T-Vector (pMD20 (Code 3270), pMD19 (Simple) (Code 3271) µî) ¶Ç´Â Mighty TA-cloning Kit (Code 6028)À» ÀÌ¿ëÇÏ¿© cloningÇÒ ¼ö ÀÖ´Ù.
¶Ç, Mighty Cloning Reagent Set (Blunt End) (Code 6027)·Î ÆòÈ° ¸»´ÜÈ­ ¹× ÀλêÈ­¸¦ 󸮸¦ ÇÏ¿©, ÆòÈ° ¸»´Ü vector¿¡µµ cloningÇÒ ¼ö ÀÖ´Ù.

Keyword :

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)