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Home > ÀüÁ¦Ç°º¸±â > PCR > Standard PCR: Ex, LA, rTaq > TaKaRa Ex Premier¢â DNA Polymerase
1st choice PCR È¿¼Ò

TaKaRa Ex Premier¢â DNA Polymerase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR370A
TaKaRa Ex Premier¢â DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
150,400¿ø 
188,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR370A_DS.v2206Da.pdf
Takara
RR370B
TaKaRa Ex Premier¢â DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (RR370A x 4)
672,000¿ø 
Takara
RR371A
TaKaRa Ex Premier¢â DNA Polymerase Dye plus
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
150,400¿ø 
188,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR371A_DS.v2206Da.pdf
Takara
RR371B
TaKaRa Ex Premier¢â DNA Polymerase Dye plus
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (RR371A x 4)
672,000¿ø 

  • GC/AT rich, long fragment, crude »ùÇà ¸ðµÎ °¡´ÉÇÑ ¸¸´É PCR È¿¼Ò
  • Æí¸®ÇÑ »ç¿ë: 2x premix ŸÀÔ, -20 ¡É¿¡¼­µµ ¾óÁö ¾Ê¾Æ Çص¿°úÁ¤ ¾øÀÌ ºü¸£°Ô »ç¿ë °¡´É
  • Dye plus ¹öÀüÀ¸·Î Àü±â¿µµ¿µµ Æí¸®ÇÏ°Ô!
Á¦Ç°¼³¸í
TaKaRa Ex Premier¢â DNA Polymerase´Â Thermococcus¼Ó °í¼¼±ÕÀÇ DNA polymerase¸¦ ±â¹ÝÀ¸·Î °³¹ßÇÑ ¥áÇü ½Å±Ô PCR È¿¼ÒÀÌ´Ù. º» Á¦Ç°Àº È¿¼Ò¿Í buffer°¡ ÃÖÀûÈ­µÇ¾î ÀÖ¾î, ÀϹÝÀûÀÎ PCR ½Ã¾àÀ¸·Î´Â ÁõÆøÀÌ ¾î·Á¿î ¼­¿­ (GC/AT rich sequence)°ú ±æÀÌ°¡ ±ä Ÿ°Ù, crude »ùÇÿ¡ ´ëÇÑ PCR ÁõÆø ¼º°ø·üÀÌ Çâ»óµÇ¾ú´Ù. ¶ÇÇÑ ¸ðµç ±¸¼ºÇ° (È¿¼Ò, buffer, dNTP Mixture µî)ÀÌ Æ÷ÇÔµÈ 2x premix ÇüÅ·Π-20¡É¿¡¼­ ¾óÁö ¾Ê¾Æ, Çص¿ °úÁ¤ ¾øÀÌ °ð¹Ù·Î primer¿Í ÁÖÇü DNA¸¦ Ãß°¡ÇÏ¿© °£ÆíÇÏ°Ô PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
Dye plus Á¦Ç° (Code RR371A/B)Àº Àü±â¿µµ¿ ½Ã Æí¸®ÇÑ loading dye (ÆĶõ»ö°ú ³ë¶õ»ö)¸¦ Æ÷ÇÔÇÏ°í ÀÖ¾î PCR ¹ÝÀÀ ÈÄ ¹Ù·Î gel¿¡ loadingÇÏ¿© Àü±â¿µµ¿À» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖ´Â È¿¼Ò´Â »ó¿Â¿¡¼­ÀÇ DNA Polymerase È°¼º ¹× 3'¡æ 5' exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â monoclonal antibody°¡ Æ÷ÇÔµÈ hot start PCR¿ë È¿¼ÒÀÌ´Ù.
¶ÇÇÑ ±³Á¤ ±â´É (proof reading)À» °¡Áø ¥áÇü ÁßÇÕ È¿¼Ò ƯÀ¯ÀÇ ³ôÀº Á¤È®¼ºµµ °®Ãß°í ÀÖÀ¸¸ç ƯÀ̼ºÀÌ ³ôÀº PCR ÁõÆø °á°ú¸¦ ¾òÀ» ¼ö ÀÖ¾î cloning¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº blunt endÀ̹ǷÎ, T-vector¿¡ cloning ÇÏ´Â °æ¿ì PCR »ê¹°ÀÇ 3¡¯ ¸»´Ü¿¡ dA¸¦ ºÎ°¡ÇÏ´Â °úÁ¤ÀÌ Æ÷ÇÔµÈ Mighty TA-cloning Reagent Set for PrimeSTAR (Code 6019)ÀÇ »ç¿ëÀ» ÃßõÇÑ´Ù.
PCR »ê¹°Àº º°µµÀÇ Á¦ÇÑÈ¿¼Ò, ligase ¹ÝÀÀ¾øÀÌ ±×´ë·Î cloning ÇÒ ¼ö ÀÖ´Â °íÈ¿À²ÀÇ PCR cloning kitÀÎ In-Fusion¢ç Snap Assembly Master Mix (Code 638947 ¿Ü)¿¡ Àû¿ëÇÒ ¼öµµ ÀÖ´Ù.
³»¿ë (200 ȸ, 50 §¡ ¹ÝÀÀ ½Ã)

RR370A

TaKaRa Ex Premier¢â DNA Polymerase (2¡¿)

1 §¢ x 5

RR371A

TaKaRa Ex Premier¢â DNA Polymerase Dye plus (2¡¿)

1 §¢ x 5

º¸Á¸ -20¡É
*»ç¿ë Àü¿¡ °¡º±°Ô ¼¯¾î¼­ spin down ÈÄ »ç¿ëÇÕ´Ï´Ù.
¿ëµµ
PCR¿¡ ÀÇÇÑ DNA ÁõÆø¿¡ »ç¿ëÇϸç ÀϹÝÀûÀÎ À¯ÀüÀÚ ÁõÆø°ú °°Àº ½ÇÇè»Ó¸¸ ¾Æ´Ï¶ó, ƯÈ÷ ¾Æ·¡¿Í °°ÀÌ ´Ù¾çÇÏ°í ¾î·Á¿î ½ÇÇè¿¡ »ç¿ëÀ» ÃßõÇÕ´Ï´Ù.
  • GC-rich, AT-rich µî ÀϹÝÀûÀÎ È¿¼Ò·Î ÁõÆøÀÌ ¾î·Á¿î Ÿ°Ù
  • 10 kb ÀÌ»óÀÇ ±ä DNA ÁõÆø
  • Áã ²¿¸® µî µ¿¹° Á¶Á÷, ½Ä¹° Á¶Á÷, Ç÷¾× »ùÇà µî crude »ùÇÃ
  • ÇÙ»êÀ» ¸¹ÀÌ Æ÷ÇÔÇÑ cDNAÀÇ ÁõÆø
  • CloningÀ» À§ÇÑ insert DNA ¶Ç´Â vectorÀÇ ÁõÆø
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- 15ºÐ ¿Ï·á! ¿øÁ¶ PCR cloning kit: In-Fusion¢ç Snap Assembly Master Mix
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit: PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- EtBrº¸´Ù ³ôÀº °¨µµÀÇ gel staining ½Ã¾à: GelStar¢ç Nucleic Acid Stain
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword :

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)