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PCR
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º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
> Bacterial 16S rDNA PCR Kit
PCR »ê¹° Sequence Çؼ®À¸·Î ¼¼±ÕÀ» ÃßÁ¤
Bacterial 16S rDNA PCR Kit
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Takara
RR180A
Bacterial 16S rDNA PCR Kit
50 ȸ
270,400¿ø
338,000¿ø
09.02 ~ 10.31
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Overview
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Taq Hot Start Version
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Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
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񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
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Hot Start Version prem..
Fast PCR¿ë premix
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Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
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³»¿ë (50 ȸ; 25 §¡ ¹ÝÀÀ°è)
Premix Ex Taq HS (2¡¿conc.)
*1
2x
625 §¡
16S rDNA Primer Mix (bacterial)
*2
10x
125 §¡
dH
2
O
650 §¡
Positive Control (E. coli DNA)
1 ng/§¡
25 §¡
Sequencing Primer F1 (bacterial)
*2
7.5 pmol/§¡
50 §¡
Sequencing Primer F2 (bacterial)
7.5 pmol/§¡
50 §¡
Sequencing Primer R1 (bacterial)
*2
7.5 pmol/§¡
50 §¡
Sequencing Primer R2 (bacterial)
7.5 pmol/§¡
50 §¡
*1
TaKaRa Ex Taq HS, reaction Buffer, dNTP mixture¸¦ Æ÷ÇÔ
*2
16S rDNA Primer Mix (bacteria)¿¡ Æ÷ÇԵǴ primer´Â Sequencing Primer F1 (bacterial) ¹×Sequencing Primer R1 (bacterial)¿Í °°Àº ¼¿ÀÌ´Ù.
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Premix Ex Taq HS´Â µ¿°á°ú À¶Çظ¦ ¹Ýº¹Çϸé È°¼ºÀÌ ÀúÇ쵃 ¼ö ÀÖ°í, À¶Çؽà °Ý·ÄÇÑ ±³¹ÝÀº ÇÇÇϽʽÿÀ.
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º» Á¦Ç°Àº ¼¼±Õ·ùÀÇ 16S rRNA À¯ÀüÀÚ³»ÀÇ Æ¯Á¤¿µ¿ª (¾à 1.5 kb)¸¦ PCR·Î ÁõÆøÇϱâ À§ÇÑ ½Ã¾à ¹× PCR ÁõÆø »ê¹°ÀÇ ¿°±â¼¿ºÐ¼®À» À§ÇÑ primer·Î ±¸¼ºµÇ¾î ÀÖ´Ù. PCR È¿¼Ò¿¡´Â TaKaRa Ex Taq HS¸¦ »ç¿ëÇϹǷÎ, cycleÀüÀÇ mis-primingÀ̳ª primer dimer¿¡ ÀÇÇÑ ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. º» Á¦Ç°À» »ç¿ëÇÏ¿© ¾òÀº ÁõÆø »ê¹°À» Á¦Ç°¿¡ Æ÷ÇÔµÈ sequencing¿ë primer·Î ¿°±â¼¿À» ºÐ¼®ÇÏ¿© µ¥ÀÌÅͺ£À̽º»óÀÇ ¼¿°úÀÇ »óµ¿°Ë»öÀ» ÅëÇØ ¼¼±Õ·ùÀÇ ºÐ·ù¸¦ ½Ç½ÃÇÑ´Ù.
ÁÖÀÇ£ºº» Á¦Ç°Àº ¼¼±Õ·ùÀÇ Á¾·ù¿¡ µû¶ó¼´Â ÀûÇÕÇÏÁö ¾Ê´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
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Fungal rDNA (ITS1) PCR Kit Fast (Code RR183A)
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PCRÁõÆø»ê¹°ÀÇ sequencing
Sequencing¿ë primer´Â 4 Á¾·ùÁß ¸Å´º¾ó¿¡ ³ªÅ¸³ primerÀÇ À§Ä¡¸¦ È®ÀÎÇÏ°í ÀûÇÕÇÑ primer¸¦ ¼±ÅÃÇÏ¿© »ç¿ëÇÑ´Ù.
Keyword :
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë Primer Set
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë Primer Set
Àå¿° Vibrio °ËÃâ¿ë Primer Set
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Primer Set VCT-1 & 2
½ÄÁßµ¶ °Ë»ç¿ë primer set¿Í ÇÔ²² »ç¿ëÇÏ´Â
Ư¼ö ¼¼±Õ °ËÃâ¿ë Positive Control Template
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë Primer Set
PCR »ê¹° Sequence Çؼ®À¸·Î ¼¼±ÕÀ» ÃßÁ¤
Bacterial 16S rDNA PCR Kit
Bacterial 16S rDNA PCR Kit Fast
Botulinus±Õ °ËÃâ¿ë Primer Set
Fungal rDNA (ITS1) PCR Kit Fast
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ)One Shot PCR Screening Kit Ver.2
Salmonella±Õ °ËÃâ¿ë Primer Set
Shigella ¹× Àå°üħÀÔ¼º ´ëÀå±Õ EIEC£©°ËÃâ¿ë Primer Set
TaKaRa źÀú±Õ PCR Detection Kit
Nested PCR·Î Mycoplasma Á¤¹Ð°ËÃâ
TaKaRa PCR Mycoplasma Detection Set
Welchii±Õ °ËÃâ¿ë Primer Set CPE-1 & 2