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PCR »ê¹° Sequence Çؼ®À¸·Î ¼¼±ÕÀ» ÃßÁ¤

Bacterial 16S rDNA PCR Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR180A
Bacterial 16S rDNA PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
270,400¿ø 
338,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR180A_Korean.pdf

³»¿ë (50 ȸ; 25 §¡ ¹ÝÀÀ°è)
Premix Ex Taq HS (2¡¿conc.) *1 2x 625 §¡
16S rDNA Primer Mix (bacterial) *2 10x 125 §¡
dH2O   650 §¡
Positive Control (E. coli DNA) 1 ng/§¡ 25 §¡
Sequencing Primer F1 (bacterial) *2 7.5 pmol/§¡ 50 §¡
Sequencing Primer F2 (bacterial) 7.5 pmol/§¡ 50 §¡
Sequencing Primer R1 (bacterial) *2 7.5 pmol/§¡ 50 §¡
Sequencing Primer R2 (bacterial) 7.5 pmol/§¡ 50 §¡

*1 TaKaRa Ex Taq HS, reaction Buffer, dNTP mixture¸¦ Æ÷ÇÔ
*2 16S rDNA Primer Mix (bacteria)¿¡ Æ÷ÇԵǴ primer´Â Sequencing Primer F1 (bacterial) ¹×Sequencing Primer R1 (bacterial)¿Í °°Àº ¼­¿­ÀÌ´Ù.
º¸Á¸ -20¡É
Premix Ex Taq HS´Â µ¿°á°ú À¶Çظ¦ ¹Ýº¹Çϸé È°¼ºÀÌ ÀúÇ쵃 ¼ö ÀÖ°í, À¶Çؽà °Ý·ÄÇÑ ±³¹ÝÀº ÇÇÇϽʽÿÀ.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ¼¼±Õ·ùÀÇ 16S rRNA À¯ÀüÀÚ³»ÀÇ Æ¯Á¤¿µ¿ª (¾à 1.5 kb)¸¦ PCR·Î ÁõÆøÇϱâ À§ÇÑ ½Ã¾à ¹× PCR ÁõÆø »ê¹°ÀÇ ¿°±â¼­¿­ºÐ¼®À» À§ÇÑ primer·Î ±¸¼ºµÇ¾î ÀÖ´Ù. PCR È¿¼Ò¿¡´Â TaKaRa Ex Taq HS¸¦ »ç¿ëÇϹǷÎ, cycleÀüÀÇ mis-primingÀ̳ª primer dimer¿¡ ÀÇÇÑ ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. º» Á¦Ç°À» »ç¿ëÇÏ¿© ¾òÀº ÁõÆø »ê¹°À» Á¦Ç°¿¡ Æ÷ÇÔµÈ sequencing¿ë primer·Î ¿°±â¼­¿­À» ºÐ¼®ÇÏ¿© µ¥ÀÌÅͺ£À̽º»óÀÇ ¼­¿­°úÀÇ »óµ¿°Ë»öÀ» ÅëÇØ ¼¼±Õ·ùÀÇ ºÐ·ù¸¦ ½Ç½ÃÇÑ´Ù.
ÁÖÀÇ£ºº» Á¦Ç°Àº ¼¼±Õ·ùÀÇ Á¾·ù¿¡ µû¶ó¼­´Â ÀûÇÕÇÏÁö ¾Ê´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
Áø±Õ·ùÀÇ °æ¿ì´Â Fungal rDNA (ITS1) PCR Kit Fast (Code RR183A)¸¦ ÀÌ¿ëÇϽʽÿÀ.
PCRÁõÆø»ê¹°ÀÇ sequencing
Sequencing¿ë primer´Â 4 Á¾·ùÁß ¸Å´º¾ó¿¡ ³ªÅ¸³­ primerÀÇ À§Ä¡¸¦ È®ÀÎÇÏ°í ÀûÇÕÇÑ primer¸¦ ¼±ÅÃÇÏ¿© »ç¿ëÇÑ´Ù.

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