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PCR Enzyme (Clontech)
> Advantage HF 2 PCR Kit
Advantage HF 2 PCR Kit
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Clontech
639123
Advantage HF 2 PCR Kit
100 rxn
877,000¿ø
,
Clontech
639124
Advantage HF 2 PCR Kit
10rxn
332,000¿ø
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Á¦Ç°¼³¸í
- highest fidelity°¡ ¿ä±¸µÇ´Â PCR¿¡ ÀûÇÕ
- Taq polymeraseÀÇ 25¹è°¡ ÀÌ»óÀÇ Á¤È®µµ¸¦ ½ÇÇö
- 3.5 kb±îÁöÀÇ genome fragment ÁõÆø °¡´É
CloningÀ̳ª µ¹¿¬º¯ÀÌ Çؼ® µî ÃÖ°íÀÇ fidelity°¡ ¿ä±¸µÇ´Â PCR¿¡ ÀÖ¾î Advantage HF 2 PCR KitÀº ÀÌ»óÀûÀÎ KitÀÌ´Ù. Advantage HF 2 KitÀÇ ¼ººÐÀº ½ÅÁßÇÏ°Ô ÃÖÀûȵǾî ÀÖÀ¸¸ç Taq polymeraseÀÇ 25¹è ¹è ÀÌ»óÀÇ ³ôÀº Á¤¹Ðµµ¸¦ ¾òÀ» ¼ö ÀÖ´Ù(±×¸² 1. Panel A). º» È¿¼Ò mix´Â 3.5 kb±îÁöÀÇ À¯ÀüÀÚ ÁõÆø¿¡ ¶Ù¾î³ ¼º´ÉÀ» ¹ßÈÖÇÑ´Ù(±×¸² 1. Panel B).
±×¸² 1. Advantage-HF 2 PCR KitÀ» »ç¿ëÇÑ ÁõÆø
Panel A. À¯ÀüÀÚ screening¿¡ ÀÖ¾î¼ Taq, Advantage 2 polymerase mix Kit, Advantage HF 2 PCR KitÀÇ fidelity¸¦ ±âÁ¸ ¹æ¹ý¿¡ µû¶ó ÃøÁ¤ÇÏ¿´´Ù(1, 2). 25 cycleÀÇ PCR¿¡¼ ÁõÆø ¼¿ 1,000 bp´ç error°¡ 0.66 bpÀÎ Taq¸¦ ±âÁØÀ¸·Î Advantage È¿¼Ò mixÀÇ ÀûÇÕµµ¸¦ Ç¥ÁØÈÇÏ¿´´Ù. Advantage 2ÀÇ error´Â 1,000 bp´ç 0.24 bpÀÌ°í Advantage HF 2ÀÇ error´Â 1,000 bp´ç 0.023 bp¿´´Ù. Panel B. ´Ù¾çÇÑ sizeÀÇ cDNA¿Í À¯ÀüÀÚ¸¦ ÁõÆø. Lane 1~2 : Marathon-ready human placenta cDNA(Cat.No. 639311)¿¡¼ÀÇ ÁõÆø. Lane 1 : 1.3 kb TFR. Lane 2 : 1.2 kb ¥â-actin. Lane 3~5 : calf thymus DNA¿¡¼ ÁõÆøÇÑ ¿©·¯ Á¾·ù size(1.0~3.5 kb)ÀÇ BPTI À¯ÀüÀÚ. Lane 6~8 : Human genome DNA¿¡¼ ÁõÆøÇÑ human cardiac ¥â-myosin chainÀÇ PCR product(0.5~1.8 kb). Lane M : 1 kb DNA size marker.
±¸¼º¹°
Advantage HF 2 PCR Kit ±¸¼º¹°
- Advantage HF 2 polymerase mix
- HF 2 PCR buffer
- PCR buffer
- HF dNTP mix(10 mM each)
- Control DNA template
- Control primer mix
- User manual(PT3317-1)
*¹ÝÀÀ¼ö´Â 50ulÀÇ ¹ÝÀÀ volumeÀ» ±âÁØÀ¸·Î ÇÕ´Ï´Ù.
º¸Á¸ -20¡É
Àû¿ëºÐ¾ß
- Cloning
- Mutation analysis
- Long and accurate PCR
- Hot start PCR
- High fidelity amplification/PCR
Âü°í¹®Çå
1. Advantage-HF PCR Kit(April 1997) Clontechniques ¥¹¥± (2):2-3.
2. Mo,J.-Y.,et al.(1991) J. Mol. Biol. 222:925-936.
¶óÀ̼¾½º È®ÀÎ »çÇ×
- Advantage 2 °ü·Ã Á¦Ç°
- PCR
- TaqStart antibody
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