1.
SpeedSTAR HS DNA Polymerase (5 U/§¡)
50 §¡
2.
10¡¿ Fast Buffer I (30 mM Mg2+ plus)
1 §¢
3.
10¡¿ Fast Buffer II (20 mM Mg2+ plus)
4.
dNTP Mixture (°¢ 2.5 mM)
800 §¡
20 mM
Tris-HCl (pH8.0)
100 mM
KCl
0.1 mM
EDTA
1 mM
DTT
0.5%
Tween 20
NP-40
50%
Glycerol
³óµµ
°¢ 2.5 mM
Çü»ó
¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9
¼øµµ
°¢ 98% ÀÌ»ó