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Fast PCR¿ë hot start È¿¼Ò

SpeedSTAR HS È¿¼Ò

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR070A
SpeedSTAR¢â HS DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
250 U
217,600¿ø 
272,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR070A_e.v1906Da.pdf
Takara
RR070B
SpeedSTAR¢â HS DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (RR070A x 4)
984,000¿ø 

Á¦Ç°¼³¸í
SpeedSTAR¢â HS DNA Polymerase´Â °í¼Ó PCR¿ëÀ¸·Î °³¹ßµÈ Hot Start DNA polymerase·Î, ÃÖÀûÈ­µÈ Fast Buffer I ¶Ç´Â IIÀ» »ç¿ëÇÏ¿© °í¼Ó PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
ÀϹÝÀûÀÎ PCR¿ë DNA polymerase´Â 1ºÐ/kb¸¦ ±âÁØÀ¸·Î extension ½Ã°£À» ¼³Á¤ÇÏÁö¸¸, º» Á¦Ç°Àº 10ÃÊ/kbÀÇ ¼³Á¤ÀÌ °¡´ÉÇÏ´Ù. ¾çÈ£ÇÑ ¹ÝÀÀ¼ºÀ» À¯ÁöÇϸ鼭 PCRÀÇ °¢ step, ƯÈ÷ extension ½Ã°£À» ´ÜÃàÇÒ ¼ö Àֱ⠶§¹®¿¡ ´Ü½Ã°£¿¡ PCR °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ¶Ç, anti-Taq antibody¸¦ ÀÌ¿ëÇÑ Hot Start È¿¼Ò·Î, cycling ÀüÀÇ mis-annealingÀ̳ª primer dimer¿¡ ÀÇÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÏ¿© ƯÀ̼ºÀÌ ³ôÀº ÁõÆø °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
³»¿ë (250 U)

1.

SpeedSTAR HS DNA Polymerase (5 U/§¡)

50 §¡

2.

10¡¿ Fast Buffer I (30 mM Mg2+ plus)

1 §¢

3.

10¡¿ Fast Buffer II (20 mM Mg2+ plus)

1 §¢

4.

dNTP Mixture (°¢ 2.5 mM)

800 §¡

º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl (pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

NP-40

50%

Glycerol

÷ºÎ dNTP Mixture Á¶¼º*

³óµµ

°¢ 2.5 mM

Çü»ó

¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9

¼øµµ

°¢ 98% ÀÌ»ó

*dATP, dCTP, dGTP, dTTPµî ¸ô È¥ÇÕ¹°·Î Èñ¼®ÇÏÁö ¾Ê°í ±×´ë·Î PCR¹ÝÀÀ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
÷ºÎ buffer¿¡ ´ëÇÏ¿©
º» Á¦Ç°¿¡´Â 2 Á¾·ùÀÇ buffer (10¡¿ Fast Buffer I, 10¡¿ Fast Buffer II)°¡ ÷ºÎµÇ¾î ÀÖ°í, ÁõÆø »ê¹°ÀÇ ±æÀÌ¿¡ µû¶ó ±¸ºÐÇÏ¿© »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÑ´Ù.
ÁõÆø»ê¹°ÀÇ ±æÀÌ 2 kb ÀÌÇÏ´Â Fast Buffer I, 2 ~ 4 kb´Â I ¶Ç´Â II, 4 kb À̻󿡴 Fast Buffer II¸¦ »ç¿ëÇϸé ÃÖÀûÀÇ È¿À²À» ¾òÀ» ¼ö ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇÏ¿©
SpeedSTAR¢â HS DNA PolymeraseÀ» ÀÌ¿ëÇÏ¿© ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3¡¯ ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector pMD20 (Code 3270) ¶Ç´Â T-vector pMD19 (Simple) (Code 3271)¿¡ cloningÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸»´Ü ÆòÈ°È­ ¹× ÀλêÈ­ ÈÄ, ÆòÈ° ¸»´Ü vector¿¡ cloningÇÒ ¼öµµ ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ½Ç¿Â º¸°ü °¡´ÉÇÑ master mix: Lyophilized HS Taq PCR Master Mix
- °íÈ¿À², °í°¨µµ, PCR È¿¼ÒÀÇ standard: TaKaRa Ex Taq¢ç
- ¿ªÀü»ç¹ÝÀÀÀ» À§ÇÑ all-in-One kit: PrimeScript¢â 1st strand cDNA Synthesis Kit
- 5x premix ¿ªÀü»ç ½Ã¾à: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword :

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)