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PCR
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Dye°¡ Æ÷ÇÔµÈ premix
> Hot Start Version premix
Dye°¡ ÷°¡µÈ °í¼º´É Hot start PCR Premix ½Ã¾à
Hot Start Version premix
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Takara
RR300A
EmeraldAmp¢ç PCR Master Mix
160 ȸ
(50 ¥ìl ¹ÝÀÀ°è)
134,400¿ø
168,000¿ø
09.02 ~ 10.31
Takara
RR300B
EmeraldAmp¢ç PCR Master Mix
800 ȸ (RR300A x 5)
670,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
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EmeraldAmp PCR Master Mix ´Â PCR¿¡ ÇÊ¿äÇÑ ÄÄÆ÷³ÍÆ®(Enzyme, buffer, dNTP Mixture µî)¿Í Àü±â¿µµ¿½Ã Æí¸®ÇÑ loading dye (ÆĶõ»ö°ú ³ë¶õ»ö)¸¦ Æ÷ÇÔÇÏ´Â 2¡¿Premix type ÀÇ Hot Start¿ë PCR È¿¼ÒÀÌ´Ù. Primer¿Í ÁÖÇü DNA¸¦ Ãß°¡ÇÏ´Â °ÍÀ¸·Î °£´ÜÈ÷ PCRÀ» ½Ç½ÃÇÒ ¼ö ÀÖ°í, ¹ÝÀÀ ÈÄ¿¡´Â ¹ÝÀÀ¾×À» Á÷Á¢ Àü±â¿µµ¿¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
¹ÝÀÀ¾×Àº ¼±¸íÇÑ ³ì»ö(Emerald Green)À¸·Î gel¿¡ ¼Õ½±°Ô loadingÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº ´ëÀå±ÕÀÇ colony PCR¿¡ ÀÇÇÑ insert üũ¿¡ ÃÖÀûÀ̾î¼, GC rich ¿µ¿ªÀÇ ÁõÆø¿¡µµ ÀûÇÕÇÑ´Ù. ¶Ç, Àΰ£ °Ô³ð DNA¸¦ ÁÖÇüÀ¸·Î ÇßÀ» °æ¿ì, 10 kb±îÁö ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
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EmeraldAmp PCR Master Mix (2¡¿Premix)
1 ml ¡¿ 4
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1 ml ¡¿ 4
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»ç¿ë ºóµµ°¡ ³ôÀ» °æ¿ì, À¶ÇØÇÑ °ÍÀº 4¡É º¸Á¸À» ÃßõÇÕ´Ï´Ù.
»ç¿ë Àü¿¡´Â mix ÈÄ ½ºÇÉ ´Ù¿îÇØ ÁֽʽÿÀ
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* DNA, cDNAÀÇ PCR ÁõÆø
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PCR °ËÁ¤
Àΰ£ °Ô³ðDNA¸¦ ÁÖÇüÀ¸·Î ÇÑ PCR ¹ÝÀÀ(ÁõÆø »ê¹°8.5 kb)¿¡¼ ¾çÈ£ÇÑ ÁõÆøÀ» È®ÀÎÇÏ°í ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇؼ º» Á¦Ç°À» ÀÌ¿ëÇؼ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº, 3'¸»´Ü¿¡ A°¡ 1¿°±â°¡ ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼, PCR »ê¹°À» ±×´ë·ÎT-Vector¿¡ Ŭ·Î´×ÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
Àü±â¿µµ¿ÀÇ loading dye
¹ÝÀÀ¾× 5 ¥ìlÀ» 1% Agarose L03·Î ¿µµ¿ÇßÀ» °æ¿ì, ÆĶõ»ö ¸¶Ä¿´Â 3¡5 kb ºÎ±Ù, ³ë¶õ ¸¶Ä¿´Â 50 bp ÀÌÇÏÀÇ À§Ä¡¿¡¼ È®ÀÎÇÒ ¼ö ÀÖ´Ù
°ü·ÃÁ¦Ç°
- ¹ü¿ëÀûÀÎ ¿Ã¶ó¿î´õ agarose:
SeaKem LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system:
FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â:
Clontech PCR Thermal Cycler GP
Keyword : PCR premix,dye plus
Dye°¡ Æ÷ÇÔµÈ premix
Dye°¡ ÷°¡µÈ °í¼º´É PCR Premix ½Ã¾à
Colony PCR¿ë premix
Dye°¡ ÷°¡µÈ °í¼º´É Hot start PCR Premix ½Ã..
Hot Start Version premix
°í¼Ó PCR ¿ë Dye plus PCR Premix
Fast PCR¿ë premix
Loading dye°¡ Æ÷ÇÔµÈ 2x premix
PCR¿ë Premix ½Ã¸®Áî