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Home > ÀüÁ¦Ç°º¸±â > PCR > RT-PCR °ü·Ã Á¦Ç° > PrimeScript¢â One Step RT-PCR Kit Ver.2
Premix·Î Æí¸®ÇÑ 1 step RT-PCR kit

PrimeScript¢â One Step RT-PCR Kit Ver.2

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR055A
PrimeScript¢â One Step RT-PCR Kit Ver.2
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
334,400¿ø 
418,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR055A_e.v2108Da.pdf
Takara
RR055B
PrimeScript¢â One Step RT-PCR Kit Ver.2
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (RR055A x 4)
1,504,000¿ø 
Takara
RR057A
PrimeScript¢â One Step RT-PCR Kit Ver.2 (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
334,400¿ø 
418,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR057A_e.v2108Da.pdf
Takara
RR057B
PrimeScript¢â One Step RT-PCR Kit Ver.2 (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (RR057A x 4)
1,504,000¿ø 

  • 1 enzyme mix, 1 buffer·Î Æí¸®ÇÑ »ç¿ë
  • ºñƯÀÌÀûÀÎ ÁõÆø ¾ïÁ¦¸¦ À§ÇÑ °í¿Â (50¡É)ÀÇ ¿ªÀü»ç¹ÝÀÀ
Á¦Ç° ¼³¸í
PrimeScript¢â One Step RT-PCR Kit Ver.2´Â RT-PCRÀ» ÇϳªÀÇ tube³»¿¡¼­ ¿¬¼ÓÀûÀ¸·Î ½Ç½ÃÇÏ´Â 1 step RT-PCR kitÀÌ´Ù. ¹ÝÀÀ µµÁß¿¡ ½Ã¾àÀ» ÷°¡ÇÒ ÇÊ¿ä°¡ ¾ø±â ¶§¹®¿¡ ¿À¿°ÀÇ °ÆÁ¤ÀÌ ¾ø´Ù. ¿ªÀü»ç¹ÝÀÀ¿¡´Â Takara Bio°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ M-MLV À¯·¡ÀÇ ¿ªÀü»çÈ¿¼Ò PrimeScript¢â RTase¸¦ »ç¿ëÇÑ´Ù. º» RTase´Â °íÂ÷ ±¸Á¶¸¦ °¡Áö´Â template RNA¿¡ ´ëÇؼ­µµ ¶Ù¾î³­ ½ÅÀ强À» ³ªÅ¸³½´Ù. ¶ÇÇÑ, PCR¿¡´Â ÁõÆø È¿À²ÀÌ ¶Ù¾î³­ Hot Start PCR È¿¼Ò TaKaRa Ex Taq¢ç HS¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
PrimeScript¢â RTase¿Í TaKaRa Ex Taq¢ç HS ¹× RNase Inhibitor¸¦ 1 step RTPCR¿ëÀ¸·Î ÃÖÀûÈ­½ÃŲ Enzyme Mix¿Í reaction buffer, dNTP Mixture ¹× One Step Enhancer SolutionÀ» Æ÷ÇÔÇÑ 2¡¿1 step buffer¸¦ »ç¿ëÇϹǷΠ¹ÝÀÀ¾× Á¦Á¶ ¶ÇÇÑ °£´ÜÈ÷ ÁøÇàÇÒ ¼ö ÀÖ´Ù.
PrimeScript¢â One Step RT-PCR Kit Ver.2 (Dye Plus)´Â ¹ÝÀÀ¾× ¾È¿¡ Àü±â¿µµ¿¿¡ ÇÊ¿äÇÑ »ö¼Ò (ÆĶõ»ö°ú ³ë¶õ»ö)¿Í ºñÁß Áõ°¡Á¦°¡ Æ÷ÇԵǾî ÀÖ¾î 1 step RT-PCR ÈÄ ¹ÝÀÀ¾×À» ±×´ë·Î agarose gel¿¡ Àü±â¿µµ¿ÇÒ ¼ö ÀÖ´Ù. ¹ÝÀÀ¾×Àº ¼±¸íÇÑ ³ì»öÀ» ¶ç°í ÀÖ¾î gel¿¡ ½±°Ô loading ÇÒ ¼ö ÀÖ´Ù.
¸¶Âù°¡Áö·Î ½ÅÀ强ÀÌ ¶Ù¾î³­ PrimeScript¢â RTase¿Í ÁõÆøÈ¿À²ÀÌ ¶Ù¾î³­ TaKaRa Ex Taq¢ç HS°¡ Æ÷ÇԵǾî ÀÖ°í, »ö¼Ò ¹× ºñÁß Áõ°¡Á¦ÀÇ Á¸Àç ÇÏ¿¡¼­ ¹ÝÀÀ¾× Á¶¼ºÀ» °íµµ¿¡ ÃÖÀûÈ­ÇÏ¿© »ö¼Ò¿Í ºñÁß Áõ°¡Á¦¸¦ Æ÷ÇÔÇÏÁö ¾Ê´Â ±âÁ¸ Á¦Ç° (Code RR055A/B)°ú µ¿µîÇÑ ¹ÝÀÀ¼ºÀ» ³ªÅ¸³½´Ù.
³»¿ë (50 §¡ ¹ÝÀÀ½Ã 50ȸ ¹ÝÀÀºÐ)
PrimeScript¢â One Step RT-PCR Kit Ver.2 (Code RR055A)
PrimeScript 1 step Enzyme Mix

100 §¡

2X 1 step Buffer

625 §¡ x 2

Control F-1 Primer*1 (20 ¥ìM)

20 §¡

Control R-1 Primer*2 (20 ¥ìM)

20 §¡

Positive Control RNA (2 x 105 copies/§¡)

20 §¡

RNase Free dH2O

625 §¡ x 2


PrimeScript¢â One Step RT-PCR Kit Ver.2 (Dye Plus) (Code RR057A)
PrimeScript 1 step Enzyme Mix

100 §¡

2X 1 step Buffer (Dye Plus)

625 §¡ x 2

Control F-1 Primer*1 (20 ¥ìM)

20 §¡

Control R-1 Primer*2 (20 ¥ìM)

20 §¡

Positive Control RNA (2 x 105 copies/§¡)

20 §¡

RNase Free dH2O

625 §¡ x 2


*1 Positive Control RNA upstream sense primer
*2 Positive Control RNA downstream anti-sense primer
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- gDNA Á¦°Å Ä÷³À¸·Î °í¼øµµ RNA Á¤Á¦: TaKaRa MiniBEST Universal RNA Extraction Kit
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

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