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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > SuperPlex¢â Premix
ºü¸£°í ±ÕÀÏÇÑ Multiplexing PCRÀ» À§ÇÑ 2X PCR Premix

SuperPlex¢â Premix

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
638543
SuperPlex¢â Premix
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
707,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x105748
Clontech
638544
SuperPlex¢â Premix
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 ȸ
2,802,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â


  • Multiplex PCR ¹ÝÀÀÁ¶°Ç ÃÖÀûÈ­¿¡ ÇÊ¿äÇÑ ½Ã°£À» ÃÖ¼ÒÈ­
  • °í¼öÀ² È¿¼Ò (High-yield enzyme) »ç¿ëÀ¸·Î ÀûÀº ¼öÀÇ PCR cycle Á¶°ÇÀ¸·Î ÁõÆø °¡´É
  • Premix ŸÀÔÀ¸·Î ½Ã°£ ¹× ½ÇÇ轺ÅÜ °¨¼Ò, Contamination µîÀÇ ¿¡·¯ ¹ß»ý °¡´É¼º ÃÖ¼ÒÈ­
Á¦Ç°¼³¸í
SuperPlex¢â Premix´Â ´ÙÄ«¶ó¹ÙÀÌ¿ÀÀÇ °í¼öÀ² PCR È¿¼ÒÀÎ TITANIUM¢ç Taq DNA Polymerase (Code 639242)¸¦ ±â¹ÝÀ¸·Î Á¦ÀÛµÈ Hot-start 2X PCR master mix ÇüÅÂÀÇ Á¦Ç°À¸·Î, Multiplex PCR »Ó¸¸ ¾Æ´Ï¶ó °í¼öÀ²ÀÇ Singleplex PCR¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù. ƯÈ÷, SuperPlex¢â Premix´Â ¼­·Î ´Ù¸¥ Ÿ°Ù¿¡ ´ëÇØ ÆíÇ⼺ÀÌ ¾ø°í ³ôÀº ÁõÆø·üÀÌ ÇÊ¿äÇÑ Multiplex PCR¿¡ ÀÌ»óÀûÀÌ´Ù.
º» Á¦Ç°Àº multiplex PCR¿¡ ÇÊ¿äÇÑ ¸ðµç Á¶¼ºÀ» Æ÷ÇÔÇÏ°í ÀÖ´Â premix ŸÀÔÀ̱⠶§¹®¿¡, ½±°í °£ÆíÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó pipetting ½ºÅÜÀ» ÃÖ¼ÒÈ­ÇÏ¿© ½ÇÇè °úÁ¤ ÁßÀÇ ¿¡·¯ ¹ß»ýÀ» °¨¼Ò½Ãų ¼ö ÀÖ´Ù.


±×¸². Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes. We compared the performance of SuperPlex Premix to four other multiplex PCR master mixes using the recommended SuperPlex Premix PCR cycle. Each lane represents the PCR products from a 20-plex reaction obtained from a different premix, visualized with ethidium bromide staining.

Lane 1: SuperPlex Premix.
Lane 2: KAPA2G Fast Multiplex Mix.
Lane 3: Platinum Multiplex PCR Master Mix.
Lane 4: NEB Multiplex PCR 5X Master Mix.
Lane 5: Qiagen Multiplex PCR Kit, without Q-solution.
Lane 6: Qiagen Multiplex PCR Kit, with Q-solution.
PCR Á¶°Ç (ÀÚ¼¼ÇÑ ³»¿ëÀº ¸Å´º¾óÀ» ÂüÁ¶ ¹Ù¶ø´Ï´Ù)
1) Singleplex PCR


2) Multiplex PCR

Àû¿ë (Multiplex PCR)
  • Pathogen detection/identification
  • High-throughput SNP genotyping
  • Mutation analysis
  • Gene deletion analysis
  • Template quantification
±¸¼ºÇ°

 

638543
(200ȸ)

638544
(1,000ȸ)

SuperPlex Premix

5 x 1§¢

5 x 5§¢

º¸Á¸ - 20¡ÆC

Keyword : multiplex,¸ÖƼÇ÷º½º,multiplexing,muptiplex pcr,pcr,pcr premix,½´ÆÛÇ÷º½º,super plex,superplex premix,SNP,SNP genotyping

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