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°íÇØ»óµµ, polyacrylamide ´ë½Å

MetaPhor Agarose

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Lonza
50181
MetaPhor¢ç Agarose
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 g
°¡°Ý¹®ÀÇ   MetaPhor Agarose(50181)_Instructions.pdf
Lonza
50180
MetaPhor¢ç Agarose
°ü·ÃÇмú ±¸¸ÅÇϱâ
125 g
°¡°Ý¹®ÀÇ  
Lonza
50184
MetaPhor¢ç Agarose
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 g
°¡°Ý¹®ÀÇ  


Application: Protein Separation in Agarose Gels
Á¦Ç°¼³¸í
MetaPhor¢ç Agarose´Â polyacrylamide¸¦ ´ë½ÅÇؼ­ »ç¿ëÇÒ ¼ö ÀÖ´Â °íÇØ»óµµÀÇ agarose·Î, Áß°£ ¼öÁØÀÇ ¿ëÇØ ¿Âµµ (75¡ÆC)¸¦ ³ªÅ¸³»¸ç, ±âÁ¸ agarose¿¡ ºñÇØ 2¹è ³ôÀº Çػ󵵸¦ °¡Áö°í ÀÖ´Ù. Fast running protocolÀ» »ç¿ëÇϸé 20 cm ±æÀÌÀÇ ¼öÁ÷Çü ¶Ç´Â ¼öÆòÇü Àü±â¿µµ¿À» ÅëÇØ 1.5½Ã°£ ³»¿¡ 1% Å©±â Â÷ÀÌÀÇ DNA ´ÜÆíÀ» ±¸ºÐÇÒ ¼ö ÀÖ´Ù (¿¹¸¦ µé¾î 200 bp DNA ´ÜÆí°ú 204 bp DNA ´ÜÆíÀ» ºÐ¸®ÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù).
2~4% MetaPhor¢ç Agarose gelÀº 4~8% polyacrylamide gel ¼öÁØÀÇ Çػ󵵸¦ °¡Á®, AMPFLP, STR ºÐ¼® ¹× tri - tetranucleotide repeat °ËÃâ¿¡ ÀûÇÕÇϸç, ÀÛÀº Å©±âÀÇ PCR »ê¹°À» ºÐ¼®ÇÏ´Â µ¥µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Analytical Specifications

Gelling temperature (3%) 

¡Â35¡ÆC

Melting temperature (3%) 

¡Â75¡ÆC

Gel strength (3%) 

¡Ã300 g/cm©÷

Ư¡
  • All Lonza Agarose is quality tested to certify performance
  • Very fine resolution of small DNA fragments
  • High gel strength
  • Low EEO
Àû¿ë
  • Small Fragments
  • PCR
Àü±â¿µµ¿ ¿¹ - 3.5% MetaPhor¢ç agarose gel

DNA ladders with 4 bp or 8 bp step sizes were prepared by ligation of Bgl II linkers. Aliquots of 0.8 ¥ìg of the ladders were separated on a 3.5% MetaPhor¢ç Agarose gel in a horizontal format and compared to an 8% polyacrylamide gel run in a vertical format in TBE buffer.
Separation range of MetaPhor¢ç Agarose
°ü·ÃÁ¦Ç°
- EtBrº¸´Ù ³ôÀº °¨µµÀÇ gel staining ½Ã¾à: GelStar¢ç Nucleic Acid Stain
- »ç¿ëÀÌ °£ÆíÇÑ premade buffer: Lonza AccuGene¢ç Buffer ½Ã¸®Áî
- Gradient PCR ±â±â: TaKaRa PCR Thermal Cycler Touch¢ç
- ³»¿­¼º gel maker set¸¦ Æ÷ÇÔÇÑ µðÁöÅÐ Àü±â¿µµ¿ ÀåÄ¡: Mupid¢ç-One

Keyword :

Agarose
>
No 1. agarose
SeaKem LE Agarose
>
1 kb ÀÌ»óÀÇ DNA ´ÜÆí ºÐ¸®¿¡
Agarose H14¡¸TAKARA¡¹
>
1 kb ÀÌ»óÀÇ DNA ´ÜÆí ºÐ¸®¿¡
Agarose L03¡¸TAKARA¡¹
>
DNA Typing ¿ë Agarose
I.D.NA Agarose
>
Isoelectric focusing ¿ë Agarose / Precast IE..
IEF : IsoGel Agarose & IsoGel Agarose IEF Plates
>
¸é¿ªÀü±â¿µµ¿ (IEF)¸¦ À§ÇÑ °íÇØ»óµµ agarose
´Ü¹éÁú ºÐ¸®¿ë Agarose
>
°íÇØ»óµµ, polyacrylamide ´ë½Å
MetaPhor Agarose
>
ªÀº ´ÜÆí (¡Â 1 kb) ÇÙ»ê ºÐ¼®¿¡ ÃÖÀû
NuSieve 3:1 Agarose
>
ªÀº ´ÜÆí ºÐ¸®¿ë Low melting Agarose
NuSieve GTG Agarose
>
Pulsed field gel electrophoresis ¿ë Agarose
SeaKem Gold Agarose
>
1 kb ÀÌ»óÀÇ DNA ´ÜÆí ºÐ¸®¿¡
SeaKem GTG Agarose
>
Elution, plaque assay¿ë low melting agarose
SeaPlaque Agarose
>
1 kb ÀÌ»óÀÇ ´ÜÆí ºÐ¸®¿ë Low melting Agarose
SeaPlaque GTG Agarose