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TaKaRa
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Å°¿öµå°Ë»ö
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  • ´ëÀüÁö»ç 
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  • ¾÷¹«½Ã°£¾È³»
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  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.

Protein Marker

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3450
Protein Molecular Weight Marker (Low)
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸºÐ
143,200¿ø 
179,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3450_DS.v2104Da.pdf
Takara
3451
Protein Molecular Weight Marker (High)
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸºÐ
143,200¿ø 
179,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3451_DS.v2104Da.pdf
Takara
3452
Protein Molecular Weight Marker (Broad)
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸºÐ
143,200¿ø 
179,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3452_DS.v2104Da.pdf
Takara
3453A
CLEARLY Protein Ladder (Unstained)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500ul
161,600¿ø 
202,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3453A_DS.v1901.pdf
Takara
3454A
CLEARLY Stained Protein Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
500ul
145,600¿ø 
182,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3454A_DS.v1901.pdf

Á¦Ç°¼³¸í
º»Á¦Ç°Àº, SDS-PAGE¿ëÀÇ ºÐÀÚ·® ¸¶Ä¿·Î ¹üÀ§°¡ ´Ù¸¥ 3°³ ŸÀÔ Low, High, Broad°¡ ÀÖ´Ù.
Low (ºÐÀÚ·®£º14.3~97.2 kDa), High (ºÐÀÚ·®£º44.3~200 kDa), Broad(ºÐÀÚ·®£º14~200 kDa)ÀÇ ¹üÀ§¿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
SDS-PAGE·Î ¿µµ¿ ÈÄ, Coomassie Brilliant Blue R-250À¸·Î ¿°»öÀ» ½Ç½ÃÇßÀ» °æ¿ì, °¢ ¹êµåÀÇ ÁøÇÔÀº °°°Ô µÈ´Ù. SDS-PAGE ¹Ì´Ï gel 1 ·¹ÀÎ´ç º» Á¦Ç°ÀÇ 20 ¹è Èñ¼®¾× 5§¡. ±× °æ¿ì, 200ȸ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë
Protein Molecular Weight Marker 50 §¡
5¡¿Loading Buffer 1 ml
1 M DTT (Dithiothreitol) 100 §¡
º¸Á¸
Protein Molecular Weight Marker, 2-ME £º£­20¡É
5¡¿Loading Buffer£º°³ºÀ ÈÄ, ½Ç¿Â º¸Á¸
³óµµ
12 ¥ìg/§¡ (TaKaRa Code 3450)
10 ¥ìg/§¡ (TaKaRa Code 3451)
18 ¥ìg/§¡ (TaKaRa Code 3452)
Çü»ó
50 mM Tris-HCl, pH6.8
1 mM EDTA
200 mM NaCl
50% glycerol
Àü±â ¿µµ¿ »çÁø
Low (TaKaRa Code 3450) High (TaKaRa Code 3451) Broad (TaKaRa Code 3452)
15% SDS-PAGE 7.5% SDS-PAGE 5-20% gradient SDS-PAGE
Á¦Ç°Áß¿¡ Æ÷ÇԵǴ ´Ü¹éÁúÀÇ Á¾·ù
Low (TaKaRa Code 3450)
´Ü¹éÁúÀÇ Á¾·ù À¯·¡ M.W.(Da)
Phosphorylase B Rabbit muscle 97,200
Serum Albumin Bovine 66,409
Ovalbumin Hen egg white 44,287
Carbonic anhydrase Bovine 29,000
Trypsin Inhibitor Soybean 20,100
Lysozyme Hen egg white 14,300
High (TaKaRa Code 3451)
´Ü¹éÁúÀÇ Á¾·ù À¯·¡ M.W.(Da)
Myosin Pig 200,000
¥â-galactosidase E. coli 116,000
Phosphorylase B Rabbit muscle 97,200
Serum Albumin Bovine 66,409
Ovalbumin Hen egg white 44,287
Broad (TaKaRa Code 3452)
´Ü¹éÁúÀÇ Á¾·ù À¯·¡ M.W.(Da)
Myosin Pig 200,000
¥â-galactosidase E. coli 116,000
Phosphorylase B Rabbit muscle 97,200
Serum Albumin Bovine 66,409
Ovalbumin Hen egg white 44,287
Carbonic anhydrase Bovine 29,000
Trypsin Inhibitor Soybean 20,100
Lysozyme Hen egg white 14,300
Aprotinin Bovine pancreas 6,500
5¡¿Loading Buffer
200 mM : Tris-HCl, pH6.8
10% : SDS
0.05% : BPB
50% : Glycerol
Protein Ladder Molecular Weight Markers
CLEARLY Protein Ladder sets´Â 10-250 kDa (unstained ladder) ¶Ç´Â 10-210 kDa (pre-stained ladder) ¹üÀ§¸¦ Ä¿¹öÇÏ´Â 11°³ÀÇ ÀçÁ¶ÇÕ ´Ü¹éÁú·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Pre-heating¾øÀÌ gel¿¡ °ð¹Ù·Î ·Îµù °¡´ÉÇϸç CLEARLY Protein Ladder (Unstained)´Â Á¤È®ÇÑ ºÐÀÚ·® È®ÀÎÀ» À§ÇÑ ±âÁØÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ CLEARLY Stained Protein Ladder´Â western blot½Ã ´Ü¹éÁúÀÇ À̵¿À» È¿°úÀûÀ¸·Î ¸ð´ÏÅÍ ÇÒ ¼ö ÀÖ´Ù. ±×·¯³ª CLEARLY Stained Protein Ladder´Â ´Ü¹éÁú¿¡ dye°¡ °áÇյǾî Àֱ⠶§¹®¿¡ °¡Àå Å« ºÐÀÚ·®ÀÇ ´Ü¹éÁúÀÇ À̵¿¿¡ ¿µÇâÀ» ³¢Ä¥ ¼öµµ Àֱ⠶§¹®¿¡ Á¤È®ÇÑ ºÐÀÚ·® ÃøÁ¤¿¡´Â ÃßõÇÏÁö ¾Ê´Â´Ù. À̶§´Â unstained ladder »ç¿ëÀ» ±ÇÀåÇÑ´Ù.


Keyword :

ºÐÀÚ·® Marker
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker
>
°í¼Ó • °í°¨µµ • °£ÆíÇÔÀ» °®Ãá Flas..
FlashGel DNA RNA Marker
>
´Ù¾çÇÑ bp ½ÇÇè¿¡ ¾È¼º¸ÂÃã
Lonza DNA Markers Ladders
>
Dye°¡ Æ÷ÇԵǾî Æí¸®ÇÑ Lonza DNA ladders
Lonza SimplyLoad DNA Ladders
>
Pulsed-field gel Àü±â¿µµ¿¿ë
Megabase DNA Ladder
>
Æí¸®ÇÏ°í Á¤È®ÇÑ Lonza protein markers
Protein Colored and Unstained Markers
>
Æí¸®ÇÏ°í Á¤È®ÇÑ Lonza RNA marker
RNA Marker (0.5 to 9 kb)
>
dsRNA MW Standard Marker
siRNA Ladder Marker