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Home > ÀüÁ¦Ç°º¸±â > Àü±â¿µµ¿ °ü·Ã > ºÐÀÚ·® Marker > DNA Ladder Markers Dye Plus (Agarose Gel Àü±â¿µµ¿ ¿ë)

DNA Ladder Markers Dye Plus (Agarose Gel Àü±â¿µµ¿ ¿ë)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3420A
20 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
168,000¿ø  3420A_DS.v2104Da.pdf
Takara
3420B
20 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3420A x 2)
320,000¿ø 
Takara
3421A
50 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
168,000¿ø  3421A_DS.v2104Da.pdf
Takara
3421B
50 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3421A x 2)
320,000¿ø 
Takara
3422A
100 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
168,000¿ø  3422A_DS.v2104Da.pdf
Takara
3422B
100 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3422A x 2)
320,000¿ø 
Takara
3423A
200 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
168,000¿ø  3423A_DS.v2104Da.pdf
Takara
3423B
200 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3423A x 2)
320,000¿ø 
Takara
3424A
250 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
142,000¿ø  3424A_DS.v2104Da.pdf
Takara
3424B
250 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3424A x 2)
268,000¿ø 
Takara
3425A
500 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
142,000¿ø  3425A_DS.v2104Da.pdf
Takara
3425B
500 bp DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3425A x 2)
268,000¿ø 
Takara
3426A
1 kb DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
142,000¿ø  3426A_DS.v2104Da.pdf
Takara
3426B
1 kb DNA Ladder (Dye Plus)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3426A x 2)
268,000¿ø 

Á¦Ç°¼³¸í
DNA Ladder (Dye Plus)¿¡´Â DNA loading dye°¡ ÷°¡µÇ¾î ÀÖ¾î ¹Ù·Î Àü±â¿µµ¿¿ë agarose gel¿¡ ·ÎµùÇÒ ¼ö ÀÖ´Ù.
°¢ Á¦Ç°Àº ¾Æ·¡ Ç¥¿Í °°Àº ladder¸¦ °®°í ÀÖ´Ù.

Á¦Ç°¸í

Ladder¹üÀ§

Ladder °£°Ý

¹êµå¼ö

ºñ °í

20 bp DNA Ladder
(Dye Plus)

20 bp ~ 500 bp

20 bp

13

20 bp~200 bp±îÁö 20 bp °£°ÝÀÇ 10 °³ ¹êµå¿Í 300 bp, 400 bp, 500 bp ÃÑ 13 °³ÀÇ ¹êµå·Î ±¸¼º.
100 bp, 200 bp ¹× 500 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

50 bp DNA Ladder
(Dye Plus)

50 bp ~ 1500 bp

50 bp

16

50 bp~500 bp±îÁö 50 bp °Å¸®ÀÇ 10 ¹êµå ¹× 600 bp, 700 bp, 800 bp, 900 bp, 1,000 bp, 1,500 bp ÃÑ 16 °³ÀÇ ¹êµå·Î ±¸¼º.
300 bp, 600 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

100 bp DNA Ladder
(Dye Plus)

100 bp ~ 1500 bp

100 bp

11

100 bp~1000 bp±îÁö 100 bp°£°ÝÀÇ ¹êµå¿Í 1500 bpÀÇ ¹êµåº¸´Ù µÈ´Ù.
500 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ¾à 3¹è ±½´Ù.

200 bp DNA Ladder
(Dye Plus)

200 bp ~ 4 kb

200 bp

13

200 bp~2 kb±îÁö 200 bp °Å¸®ÀÇ 10 ¹êµå ¹× 2.5 kb, 3 kb, 4 kb ÃÑ13 °³ÀÇ ¹êµå·Î ±¸¼º.
1 kb°ú 2 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

250 bp DNA Ladder
(Dye Plus)

250 bp ~ 4.5 kb

250 bp

8

250 bp~1,000 bp±îÁö 250 bp °£°ÝÀÇ 4 °³ÀÇ ¹êµå¿Í 1,500 bp, 2,250 bp, 3,000 bp, 4,500 bp ÃÑ 8 °³ÀÇ ¹êµå·Î ±¸¼º.
1,000 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ¾à 3 ¹è ±½´Ù.

500 bp DNA Ladder
(Dye Plus)

500 bp ~ 5 kb

500 bp

10

1,000 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ¾à 3 ¹è ±½´Ù.
2 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

1 kb DNA Ladder
(Dye Plus)

1 kb ~ 10 kb

1 kb

10

5 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

* Loading dye¸¦ Æ÷ÇÔÇÏÁö ¾Ê´Â ladder´Â DNA Markers ÆäÀÌÁö (¸µÅ©)¸¦ Âü°íÇϼ¼¿ä.
DNA Ladder Marker (Dye Plus) ¸ð½Äµµ
Çü»ó
1. 100 bp DNA Ladder (Dye Plus)

10 mM

Tris-HCl, pH8. 0

1 mM

EDTA

6£¥

Glycerol

0.1£¥

SDS

0.005£¥

Bromophenol Blue


2. 100 bpÀÌ¿ÜÀÇ DNA Ladder (Dye Plus)

10 mM

Tris-HCl, pH8. 0

6 mM

EDTA

6£¥

Glycerol

0.0083£¥

Bromophenol Blue

0.0033£¥

Xylene Cyanol

º¸Á¸
-20¡É (¿ëÇØ ÈÄ 4 ¡É¿¡¼­ ÀúÀå; µ¿°áÀ¶ÇØ ºÒ°¡)
»ç¿ë»óÀÇ ÁÖÀÇ
»ó±â Á¦Ç°Àº Agarose gel Àü±â¿µµ¿¿ëÀ¸·Î °³¹ßµÈ °ÍÀ¸·Î, ´Ù¸¥ ¿ëµµ·Î »ç¿ëÇÏ¸é ¿µµ¿ ÆÐÅÏÀÌ ´Þ¶óÁú ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
* Lonza ¸íÇ° agarose
   - ¹ü¿ëÀûÀÎ SeaKem¢ç LE Agarose (Code 50004 ¿Ü)
   - AFLP, RAPD ultrafine (20 ~ 800 bp) ÇØ»óµµÀÇ MetaPhor¢ç Agarose (Code 50180 ¿Ü)
   - Low melting »ùÇÃȸ¼ö¿¡ ÃÖÀû SeaPlaque¢ç Agarose (Code 50100 ¿Ü)

* ´Ù¾çÇÑ PCR È¿¼Ò - PCR Enzyme Guide ºê·¯¼Å ¹Ù·Î°¡±â (Ŭ¸¯)

Keyword :

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