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Home > ÀüÁ¦Ç°º¸±â > Àü±â¿µµ¿ °ü·Ã > ºÐÀÚ·® Marker > DNA Other Markers (Agarose Gel Àü±â¿µµ¿ ¿ë)

DNA Other Markers (Agarose Gel Àü±â¿µµ¿ ¿ë)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3409A
20 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ¥ìg
(100 ȸ)
134,400¿ø 
168,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3409A_DS.v2406Da.pdf
Takara
3409B
20 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ¥ìg¡¿2 (3409A x 2)
320,000¿ø 
Takara
3407A
100 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl
(100 ȸ)
96,000¿ø 
120,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3407A_DS.v2406Da.pdf
Takara
3407B
100 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿2 (3407A x 2)
228,000¿ø 
Takara
3410A
200 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl
(100 ȸ)
124,000¿ø 
155,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3410A_DS.v2406Da.pdf
Takara
3410B
200 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl¡¿2 (3410A x 2)
296,000¿ø 
Takara
3411A
500 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl
(100 ȸ)
110,400¿ø 
138,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3411A_DS.v2406Da.pdf
Takara
3411B
500 bp DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl¡¿2 (3411A x 2)
260,000¿ø 
Takara
3412A
1 kb DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl
(100 ȸ)
110,400¿ø 
138,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3412A_DS.v2406Da.pdf
Takara
3412B
1 kb DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ¥ìl¡¿2 (3412A x 2)
260,000¿ø 
Takara
3413A
2.5 kb DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ¥ìg
(100 ȸ)
188,800¿ø 
236,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3413A_DS.v1901.pdf
Takara
3413B
2.5 kb DNA Ladder
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ¥ìg¡¿2 (3413A x 2)
448,000¿ø 

Á¦Ç°¼³¸í
°¢ Á¦Ç°Àº ¾Æ·¡ Ç¥¿Í °°Àº ladder¸¦ °®°í ÀÖ´Ù.

Á¦Ç°¸í

Ladder¹üÀ§

Ladder °£°Ý

¹êµå¼ö

ºñ °í

20 bp DNA Ladder

20 bp~500 bp

20 bp

13

20 bp~200 bp±îÁö 20 bp °£°ÝÀÇ 10°³ÀÇ ¹êµå ¹× 300 bp, 400 bp, 500 bp ÃÑ 13°³ÀÇ ¹êµå·Î ±¸¼º.
100 bp, 200 bp ¹× 500 bpÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

100 bp DNA Ladder

100 bp~1500 bp

100 bp

11

100 bp~1000 bp±îÁö 100 bp°£°ÝÀÇ ¹êµå¿Í 1500 bpÀÇ ¹êµå·Î ±¸¼º.
5 §¡ (650 ng) »ç¿ëÇÑ °æ¿ì 500 bpÀÇ ¹êµå´Â ¾à 150 ng, ´Ù¸¥ ¹êµå´Â ¾à 50 ng ÀÌ´Ù.

200 bp DNA Ladder

200 bp~4 kb

200 bp

13

200 bp~2 kb±îÁö 200 bp°£°ÝÀÇ 10°³ÀÇ ¹êµå ¹× 2.5 kb, 3 kb, 4 kb ÃÑ 13°³ÀÇ ¹êµå·Î ±¸¼º
1 kb¿Í 2 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

500 bp DNA Ladder

500 bp~5 kb

500 bp

10

2 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

1 kb DNA Ladder

1 kbp~10 kb

1 kb

10

5 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.

2.5 kb DNA Ladder

2.5~>35 kb

2.5 kb

£¾14

10 kbÀÇ ¹êµå´Â ´Ù¸¥ ¹êµåº¸´Ù ±½´Ù.


* Loading dye°¡ Æ÷ÇÔµÈ ladder´Â DNA Ladder Markers Dye Plus ÆäÀÌÁö (¸µÅ©)¸¦ Âü°íÇϼ¼¿ä.
DNA Ladder Marker ¸ð½Äµµ
³óµµ

20 bp, 200 bp, 500 bp, 1 kbÀÇ °¢ DNA Ladder

200 ng/§¡

100 bp DNA Ladder

650 ng/5§¡

2.5 kb DNA Ladder

100 ng/§¡

Çü»ó

10 mM

Tris-HCl(pH8.0; 100 bp DNA Ladder´Â pH7.4)

1 mM

EDTA

º¸Á¸
-20¡É
2.5 kb DNA Ladder¿¡¸¸ 4¡É
(÷ºÎÇÑ 6¡¿Loading Buffer´Â °³ºÀ ÈÄ »ó¿Â º¸Á¸)
ºñ°í
6¡¿Loading Buffer*1 §¢ ÷ºÎ
*6¡¿Loading BufferÁ¶¼º
36£¥

Glycerol

30 mM

EDTA

0.05£¥

Bromophenol Blue

0.035£¥

Xylene Cyanol

»ç¿ë»óÀÇ ÁÖÀÇ
»ó±â Á¦Ç°Àº Agarose gel Àü±â¿µµ¿¿ëÀ¸·Î °³¹ßµÈ °ÍÀ¸·Î, ´Ù¸¥ ¿ëµµ·Î »ç¿ëÇÏ¸é ¿µµ¿ ÆÐÅÏÀÌ ´Þ¶óÁú ¼ö ÀÖ´Ù.
ƯÈ÷ 20 bp DNA Ladder´Â denatured polyacrylamide gel¿¡ Àü±â¿µµ¿À» ÇÏ¸é ¹êµå°¡ 2ÁßÀ¸·Î °ãÃÄÁö¹Ç·Î »ç¿ëÇÒ ¼ö ¾ø´Ù. Non-denatured polyacrylamide gelµµ ¿µµ¿Á¶°Ç¿¡ µû¶ó¼­ µ¿ÀÏÇÑ °æÇâÀ» º¸À̹ǷΠ»ç¿ë¿¡´Â Àû´çÇÏÁö ¾Ê´Ù.
°ü·ÃÁ¦Ç°
* Lonza ¸íÇ° agarose
   - ¹ü¿ëÀûÀÎ SeaKem¢ç LE Agarose (Code 50004 ¿Ü)
   - AFLP, RAPD ultrafine (20 ~ 800 bp) ÇØ»óµµÀÇ MetaPhor¢ç Agarose (Code 50180 ¿Ü)
   - Low melting »ùÇÃȸ¼ö¿¡ ÃÖÀû SeaPlaque¢ç Agarose (Code 50100 ¿Ü)

* ´Ù¾çÇÑ PCR È¿¼Ò - PCR Enzyme Guide ºê·¯¼Å ¹Ù·Î°¡±â (Ŭ¸¯)

Keyword :

ºÐÀÚ·® Marker
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker
>
°í¼Ó • °í°¨µµ • °£ÆíÇÔÀ» °®Ãá Flas..
FlashGel DNA RNA Marker
>
´Ù¾çÇÑ bp ½ÇÇè¿¡ ¾È¼º¸ÂÃã
Lonza DNA Markers Ladders
>
Dye°¡ Æ÷ÇԵǾî Æí¸®ÇÑ Lonza DNA ladders
Lonza SimplyLoad DNA Ladders
>
Pulsed-field gel Àü±â¿µµ¿¿ë
Megabase DNA Ladder
>
Æí¸®ÇÏ°í Á¤È®ÇÑ Lonza protein markers
Protein Colored and Unstained Markers
>
Æí¸®ÇÏ°í Á¤È®ÇÑ Lonza RNA marker
RNA Marker (0.5 to 9 kb)
>
dsRNA MW Standard Marker
siRNA Ladder Marker