FlashGel¢â System for RNA
Precast gel cassettes ÇÙ»ê Àü±â ¿µµ¿ ½Ã½ºÅÛÀ¸·Î ºü¸¥ ½Ã°£ ³»¿¡ °£ÆíÇÏ°Ô RNA ºÐ¸® ¹× purity È®ÀÎÀÌ °¡´ÉÇÏ´Ù. FlashGel¢â RNA Cassettes¿¡´Â
precast agarose gel, buffer, gel ¿°»ö ½Ã¾àÀÌ ¹Ì¸® Æ÷ÇԵǾî Àֱ⠶§¹®¿¡ cassettes¸¦ dock º»Ã¼¿¡ ÀåÂøÇÏ¿© »ùÇà ·Îµù¸¸ Çϸé Àü±â¿µµ¿À» ÇÒ ¼ö ÀÖ´Ù.
Á¦Ç°ÄÚµå |
Á¦Ç°¸í |
±¸¼º |
57024 |
FlashGel¢â System for RNA Starter Pack |
FlashGel¢â RNA Cassettes 1.2% (12+1 well)), FlashGel¢â RNA marker, Formaldehyde Sample Buffer, Molecular Biology Water |
57065 |
FlashGel¢â Camera Pack |
FlashGel¢â Dock, FlashGel¢â Camera |
Ư¡
- 5ºÐ¸¸¿¡ Àü±â ¿µµ¿ ¿Ï·á
FlashGel RNA SystemÀº ¿ÜºÎ Àü¿ø °ø±Þ ÀåÄ¡·ÎºÎÅÍ 225 V±îÁö Àü¾ÐÀ» °É ¼ö ÀÖÀ¸¸ç, 0.5-9 kb ±îÁöÀÇ RNA fragment ºÐ¸®°¡
3-8 ºÐÀÌ¸é ¿Ï·áµÈ´Ù (single-tier: 225 V 4-8 ºÐ, double-tier: 225 V 3-5 ºÐ).
* ¹êµå¸¦ ¼±¸íÈ÷ °üÂûÇÏ·Á¸é, Àü±â¿µµ¿ ÈÄ 10ºÐ Á¤µµ incubation½Ã°£À» ÁÖ´Â °ÍÀÌ ÁÁ´Ù.
Cassettes 1.2% agarose, 12+1 well ÀÌ¿ëÇÑ Àü±â ¿µµ¿ °á°ú
Cassette run for 8 minutes at 225V and photographed 20 minutes post-run.
Sample lane
1, 6, 11£ºDNA Marker (for visualization during run)
2, 7, 12£ºRNA Maker (100 ng)
3, 8, 13£ºE. coli total RNA (100 ng)
4, 9£ºS. cerevisiae total RNA (100 ng)
5, 10£ºMouse Thymus total RNA (100 ng)
- °£ÆíÇÑ Cassettes (RNase free)
FlashGel Cassettes¿¡´Â precast agarose gel, buffer, gel ¿°»ö ½Ã¾àÀÌ ¹Ì¸® Æ÷ÇԵǾî ÀÖ´Ù. °ÖÀ» ÁغñÇÏ´Â ¹ø°Å·Î¿ò¾øÀÌ
¸î ºÐ¾È¿¡ °á°ú¸¦ ¾òÀ» ¼ö Àֱ⠶§¹®¿¡ ÃßÃâÇÑ total RNA È®Àο¡ ÃÖÀûÀÌ´Ù.
- °í°¨µµ °ËÃâ
FlashGel System¿¡¼´Â 10 ng RNA/band±îÁöÀÇ °ËÃâÀÌ °¡´ÉÇϱ⠶§¹®¿¡, ÀûÀº ¾çÀÇ »ùÇ÷εµ ¹êµå È®ÀÎÀÌ °¡´ÉÇÏ´Ù.
»ç¾ç
- FlashGel Dock*
Dock size£º134 mm¡¿120 mm¡¿54 mm
* º°µµ Àü¿ø °ø±Þ ÀåÄ¡°¡ ÇÊ¿ä (225 VÀÇ °ø±Þ ´É·Â)
- FlashGel RNA Cassettes*
Cassettes size£º115 mm¡¿107 mm¡¿17 mm
Separation range£º 0.5-9 kb
»ùÇà ·Îµù·®£º5 §¡/wellÀÌÇÏ
* Cassettes Æó±â ¹æ¹ý : EtBr ó¸® ¹æ¹ý°ú µ¿ÀÏÇÏ°Ô Æó±â, Cassettes ¿Í °ÖÀº ºÐ¸® ºÒ°¡
- FlashGel RNA Marker, 0.5 kb - 9 kb
Formaldehyde Sample Buffer (Code 50571)°ú È¥ÇÕÇÏ¿© »ç¿ëÇÑ´Ù (65¡É 5ºÐ).
Band size£º0.5, 1.0, 1.5, 3.0, 5.0, 9.0 kb
- Formaldehyde Sample Buffer
º¯¼º RNA »ùÇÃÀ» Á¶Á¦ ½Ã »ç¿ëÇϸç, RNA ¿ë¾×°ú °°Àº ¾çÀ» È¥ÇÕÇØ »ç¿ë. Bromophenol Blue, Xylene CyanolÀ» Æ÷ÇÔ.
- FlashGel Loading Dye (5X)
¹Ìº¯¼º RNA »ùÇÃÀ» Á¶Á¦ ½Ã »ç¿ëÇÑ´Ù.
- AccuGENE Molecular Biology Water
DNase, RNase free water
º¸Á¸
- FlashGel RNA Cassettes : ½Ç¿Â(18-26¡É), * Á¦Á¶ ÈÄ 3°³¿ù
*½Ç¿ÂÀÌ 26¡É¸¦ ³Ñ´Â °æ¿ì´Â 4¡É º¸Á¸À» ÃßõÇÕ´Ï´Ù. »ç¿ë Àü¿¡´Â cassettes¸¦ ½Ç¿ÂÀ¸·Î À¯ÁöÇÑ ÈÄ »ç¿ëÇØ ÁÖ¼¼¿ä.
¡Ø FlashGelÀº Lonza»çÀÇ µî·Ï»óÇ¥ÀÔ´Ï´Ù.
RNA Sample Buffers °³¿ä
- Glyoxal Sample Buffer
Lonza Glyoxal Sample Buffer´Â RNA º¯¼ºÁ¦·Î MOPS buffer ±â¹ÝÀÇ gel¿¡ »ç¿ëÇÒ ¶§ ÀûÇÕÇÏ´Ù. northern blotting¿¡´Â
ÀûÇÕÇϳª RNA »ùÇÃÀ» ȸ¼öÇØ¾ß ÇÑ´Ù¸é ÀûÇÕÇÏÁö ¾Ê´Ù. Glyoxal sample buffer´Â MOPS Buffer¿¡ Glyoxal°ú DMSO¸¦ ÇÔÀ¯Çϰí ÀÖ´Ù.
- Formaldehyde Sample Buffer
Formaldehyde º¯¼ºÀº agarose gel¿¡¼ »ùÇÃÀ» ȸ¼öÇØ¾ß ÇÒ ¶§ ÀûÇÕÇÏ´Ù. Lonza Formaldehyde Sample Buffer´Â MOPS buffer¿¡
formaldehyde, formamide¿Í dye¸¦ ÇÔÀ¯Çϰí ÀÖ´Ù.
°ü·ÃÁ¦Ç°
»ç¿ëÀÌ °£ÆíÇÑ Lonza AccuGene Buffer ½Ã¸®Áî ¹Ù·Î°¡±â
´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÑ º£½ºÆ®¼¿·¯ SeaKem Agarose ¹Ù·Î°¡±â
EtBrº¸´Ù ³ôÀº °¨µµ·Î staining °¡´ÉÇÑ GelStar Nucleic Acid Stain ¹Ù·Î°¡±â
°í¼Ó ¡¤ °£Æí ¡¤ °í°¨µµ¸¦ µ¿½Ã¿¡ ½ÇÇöÇÑ Àü±â¿µµ¿ ½Ã½ºÅÛ FlashGel System for DNA ¹Ù·Î°¡±â
³î¶ó¿ï Á¤µµ·Î °£Æí, ½Å¼ÓÇÑ DNA ´ÜÆí ȸ¼ö ½Ã½ºÅÛ FlashGel System for DNA Recovery ¹Ù·Î°¡±â