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Home > ÀüÁ¦Ç°º¸±â > Àü±â¿µµ¿ °ü·Ã > ºÐÀÚ·® Marker > DNA Digested Markers (Agarose Gel Àü±â¿µµ¿ ¿ë)

DNA Digested Markers (Agarose Gel Àü±â¿µµ¿ ¿ë)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3401
¥ë-EcoT14 I digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg
62,400¿ø 
78,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3401_DS.v1901Da.pdf
Takara
3408
¥ë-EcoT14 I/Bgl II digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg
71,200¿ø 
89,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3408_DS.v1901Da.pdf
Takara
3402
¥ë-BstP I digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg
62,400¿ø 
78,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3402_DS.v1901Da.pdf
Takara
3403
¥ë-Hind III digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg
62,400¿ø 
78,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3403_DS.v1901Da.pdf
Takara
3405A
¥õX174 Hae III digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
80,800¿ø 
101,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3405A_DS.v1901Da.pdf
Takara
3405B
¥õX174 Hae III digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg (3405A x 5)
455,000¿ø 
Takara
3406A
¥õX174 Hinc II digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
80,800¿ø 
101,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3406A_DS.v1901Da.pdf
Takara
3406B
¥õX174 Hinc II digest
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg (3406A x 5)
455,000¿ø 
¢ß¾ßÄí¸£Æ® º»»ç
3404A
pHY Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
145,600¿ø 
182,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3404A_DS.v2104Da.pdf
¢ß¾ßÄí¸£Æ® º»»ç
3404B
pHY Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìg (3404A x 5)
820,000¿ø 

³óµµ : 200~800 ng/§¡
Çü»ó
10 mM Tris-HCl (pH8.0)
1 mM EDTA
º¸Á¸ -20¡É
6¡¿ Loading Buffer 1 ml ÷ºÎ
36% Glycerol
30 mM EDTA
0.05% Bromophenol Blue
0.05% Xylene Cyanol
Á¦Ç°¼³¸í
¡¤ ¥ëDNA digest´Â bacteriophage ¥ëcI857 Sam 7ÀÇ DNA¸¦ Á¦ÇÑÈ¿¼Ò EcoT14 I, BstP I, Hind III·Î °¢°¢ Àý´ÜÇÑ °ÍÀÌ´Ù.
  ¥ë-EcoT14 I/ Bgl II digest´Â ¥ëcI857 Sam 7ÀÇ DNA¸¦ Á¦ÇÑÈ¿¼Ò EcoT14 I, Bgl II·Î ¿ÏÀüºÐÇØÇÑ °ÍÀÇ È¥ÇÕ¹°ÀÌ´Ù.
¡¤ ¬æX174 digest´Â bacteriophage ¬æX174am3ÀÇ DNA¸¦ Á¦ÇÑÈ¿¼Ò Hae III, Hinc II·Î °¢°¢ Àý´ÜÇÑ °ÍÀÌ´Ù.
¡¤ pHY Marker, pHY300 PLKÀÇ Hind IIIÀÇ ºÐÇع°°ú Hae IIIÀÇ ºÐÇع°, pHY300 PLK dimer plasmidÀÇ Hae III ºÐÇع°À» È¥ÇÕÇÑ °ÍÀÌ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
¥ëDNA digestÀÇ ¸»´Ü ´ÜÆíÀº COS¸»´ÜÀ¸·Î °áÇյǾî ÀÖ¾î Àü±â¿µµ¿ Àü¿¡ ¿­Ã³¸® (60¡É, 5ºÐ)°¡ ÇÊ¿äÇÏ´Ù. ±× °æ¿ì ¿°ÀÌ Á¸ÀçÇÏÁö ¾ÊÀ¸¸é DNAÀÇ Tm (melting temperature)°ªÀÌ ³·¾ÆÁ® ´ÜÆí¿¡ µû¶ó¼­´Â º¯¼ºµÉ ¼ö ÀÖ´Ù. Èñ¼®ÇÏ´Â °æ¿ìµµ TE³ª TEN (TE + NaCl) ¹öÆÛ¸¦ »ç¿ëÇÏ°í ¿° Á¸ÀçÇÏ¿¡¼­ ¿­Ã³¸® ÇØ¾ß ÇÑ´Ù.

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