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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Takara) > PrimeScript¢â 1st strand cDNA Synthesis Kit
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ÇÕ¼º¿ë Kit

PrimeScript¢â 1st strand cDNA Synthesis Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6110A
PrimeScript¢â 1st strand cDNA Synthesis Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
244,800¿ø 
306,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6110A_e.v2203Da.pdf
Takara
6110B
PrimeScript¢â 1st strand cDNA Synthesis Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (6110A x 4)
980,000¿ø 


  • High fidelity: ³ôÀº Á¤È®µµÀÇ cDNA ÇÕ¼º, Cloning ½ÇÇè¿¡ ÃÖÀû
  • Strong strand displacement & elongation activity: Full length cDNA ÇÕ¼º¿¡ ÃÖÀû (~ 12 kb)
  • ³·Àº ¹ÝÀÀ¿Âµµ (42¡É)¿¡¼­ GC-rich ¹× °íÂ÷±¸Á¶ RNA »ùÇñîÁö Àû¿ë (RNAÀÇ degradation ÃÖ¼ÒÈ­)
  • ³ôÀº ƯÀ̼º, ³ôÀº sensitivity: non-specific annealing ¾øÀÌ ¼Ò·® RNA »ùÇà Àû¿ë °¡´É
  • cDNA ÇÕ¼ºÀ» À§ÇÑ All-in-One Kit (oligo dT primers, random 6mer Æ÷ÇÔ)
Á¦Ç°¼³¸í
ÀÌ Á¦Ç°Àº PrimeScript RTase¸¦ ÀÌ¿ëÇÏ¿© total RNA ȤÀº Poly A+ RNA·Î ºÎÅÍ 1st strand cDNA¸¦ ÇÕ¼ºÇϱâ À§ÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ all-in one kitÀÌ´Ù.
´ÙÄ«¶ó¿¡¼­ °³¹ßÇÑ M-MLV(Moloney Murine Leukemia virus) À¯·¡ÀÇ Reverse Transcriptase PrimeScript RTase¸¦ »ç¿ëÇÏ¿´±â¿¡ ½ÅÀ强ÀÌ ¸Å¿ì ¶Ù¾î¸ç ÃÖ´ë 12 kb±îÁö cDNA ÇÕ¼ºÀÌ È®ÀεǾú´Ù. ¶ÇÇÑ ÀÌ È¿¼Ò´Â cDNA ÇÕ¼ºÀÌ ¾î·Á¿î GC richÀÇ RNA¿Í °íÂ÷±¸Á¶ÀÇ RNA¿¡ ´ëÇؼ­µµ ³·Àº ¹ÝÀÀ¿Âµµ (42 ¡É)¿¡¼­ È¿À²ÀûÀ¸·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÏ´Ù.
ÀÌ·¸°Ô ÇÕ¼ºµÈ 1st strand cDNA´Â 2nd strand ÇÕ¼º, hybridization, PCR µî ´Ù¾çÇÑ ¿ëµµ·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50ȸ ºÐ·®)

PrimeScript RTase (200 U/§¡)*1

 

50 §¡

5×PrimeScript Buffer

 

200 §¡

RNase Inhibitor (40 U/§¡}

 

25 §¡

dNTP Mixture (10 mM each)

 

50 §¡

Oligo dT Primer (50 μM)

 

50 §¡

Random 6 mers (50 μM)

 

100 §¡

RNase free dH2O

 

1 §¢

º¸Á¸ -20¡É
¿ëµµ
- 1st strand cDNA ÇÕ¼º
- cDNA probe Á¦ÀÛ
- RT-PCR
- Full lengthÀÇ cDNA ÇÔ·®ÀÌ ³ôÀº cDNA ¶óÀ̺귯¸® Á¦ÀÛ.
1st strand cDNA ÇÕ¼º´É ºñ±³

PrimeScript RTase´Â ±æÀÌ°¡ ±ä cDNA ÇÕ¼º´É·ÂÀÌ ¶Ù¾î³ª°í, background°¡ ÀûÀ¸¸ç full length cDNAÀÇ ºñÀ²ÀÌ ³ô°Ô ÇÕ¼ºµÈ´Ù´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù (°¢ Á¦Ç° ¸Þ´º¾ó Á¶°Ç¿¡¼­ ¹ÝÀÀ).
°ü·ÃÁ¦Ç°
- High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
- PCR, cloning, sequencingµîÀÇ ½ÇÇè¿ë cDNA ÇÕ¼ºÀÇ ³¡ÆÇ¿Õ, PrimeScript™ IV 1st strand cDNA Synthesis Mix (Code 6215A)
- Real-Time PCR¿ë cDNA¸¦ gDNA ¿À¿° ¾øÀÌ ÇÕ¼º, PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Code RR047A)
- gDNA Eraser ColumnÀ¸·Î gDNA ¿À¿° ¾øÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ RNA Á¤Á¦, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
- ½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇϱâ

Keyword : Ŭ·Î´×, cloning, infusion, qPCR, real time PCR, cDNA

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