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Reverse Transcriptase XL (AMV)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Life Sciences
2620A
Reverse Transcriptase XL (AMV)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 U
256,800¿ø 
321,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
³³±âÁö¿¬ (2022.7. ±âÁØ)
2620A_DS.v2109.pdf

*º» Á¦Ç°Àº ±¸¸Å Àü ¹Ýµå½Ã °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î ³³±â È®ÀÎ ¹Ù¶ø´Ï´Ù.
Á¦Ç°¼³¸í
ÀÌ Á¦Ç°Àº Avian myeloblastosis virus (AMV)¿¡¼­ Á¤Á¦µÈ ºÐÀÚ·®ÀÌ ¾à 157,000ÀÇ αβÇü holoenzymeÀÇ ¿ªÀü»ç È¿¼ÒÀÌ´Ù. Nuclease-free·Î °í¼øµµ Á¤Á¦µÇ¾úÀ¸¸ç, 10 kbÀÇ RNA¸¦ templateÀ¸·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÏ´Ù´Â °ÍÀÌ È®ÀεǾú°í, cDNA ÇÕ¼ºÀÇ È°¼º»Ó ¾Æ´Ï¶ó RNase H È°¼º ¿ª½Ã °¡Áö°í ÀÖ´Ù.
º¸Á¸ -80¡É
- ¸î Â÷·ÊÀÇ freeze/thaw °úÁ¤ÀÇ ¹Ýº¹Àº È°¼º¿¡ °ÅÀÇ ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÁö¸¸ °¡±ÞÀû ÇÇÇÏ´Â °ÍÀ» ÃßõÇÑ´Ù.
- ´Ü±â°£ (6°³¿ù ÀÌÇÏ) º¸°üÇÏ´Â °æ¿ì -20¡É º¸Á¸µµ °¡´ÉÇÏ´Ù.
³óµµ 15 ~ 40 U/§¡
Çü»ó

200 mM

 

Potassium Phosphate, pH 7.2

2 mM

 

DTT

0.2 %

 

Triton X-100

50 %

 

Glycerol (v/v)

÷ºÎµÈ BufferÀÇ Á¶¼º (10X/5X Reaction Buffer)

250 mM

 

Tris-HCl, pH 8.3

500 mM

 

KCl

20 mM

 

DTT

50 mM

 

MgCl2


* ±æÀÌ°¡ ±ä cDNA ÇÕ¼º½Ã¿¡´Â 10X·Î Èñ¼®Çؼ­ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀå µå¸³´Ï´Ù.
È°¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

50 mM

 

Tris-HCl, pH 8.3

40 mM

 

KCl

6 mM

 

MgCl2

4 mM

 

DTT

0.4 mM

 

poly (rA)¡¤(dT)12-18

05. mM

 

[3H]dTTP

2~4 U

 

RTase XL (AMV)

¿ëµµ
- 1st strand cDNAÀÇ ÇÕ¼º
- RNA-PCR
- cDNA probe Á¦ÀÛ
±â¿ø
- Avian myeloblastosis virus (AMV)
ÀϹÝÀûÀÎ ¼ºÁú
- ºÐÀÚ·®
   αβÇü:157,000 Da
°ü·ÃÁ¦Ç°
- High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
- Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø High fidelity ¿ªÀü»ç È¿¼Ò standard, PrimeScript™ 1st strand cDNA Synthesis Kit (Code 6110A)
- ¾Ç¼¼¼­¸® ´Ü¹éÁú·Î °íÂ÷±¸Á¶ RNA Àû¿ë¼ºÀÌ °­È­µÈ PrimeScript™, PrimeScript™ II 1st strand cDNA Synthesis Kit (Code 6210A)
- gDNA Á¦°Å ÈÄ Real-Time PCR¿ë cDNA ÇÕ¼º, PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Code RR047A)

Keyword :

Reverse Transcription (Takara)
>
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
>
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
>
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
>
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
>
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
>
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
>
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer