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Reverse Transcriptase XL (AMV)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Life Sciences
2620A
Reverse Transcriptase XL (AMV)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 U
300,000¿ø  2620A_DS.v2109.pdf

³óµµ 15~40 U/§¡
Çü»ó
200 mM Potassium Phosphate (pH7.2)
2 mM DTT
0.2% Triton X-100
50% Glycerol
º¸Á¸
- 80¡£C µ¿°áº¸Á¸ (µ¿°áÀ¶ÇØ ¹Ýº¹¿¡ µû¸¥ Ȱ¼ºÀúÇÏ´Â ¾øÀ¸³ª °¡´ÉÇÑ ÇÑ ÇÇÇÒ °Í)
- ´Ü±â°£ÀÎ °æ¿ì -20¡É¿¡ º¸Á¸ °¡´É.
÷ºÎ Buffer Á¶¼º (cDNA ÇÕ¼º¿ë, 10¡¿/ 5¡¿)
250 mM Tris-HCl (pH8.3)
500 mM KCl
20 mM DTT
50 mM MgCl2
* Full length cDNA ÇÕ¼º½Ã¿¡´Â ÷ºÎ ¹öÆÛ¸¦ 1/10·Î Èñ¼®ÇÏ¿© »ç¿ëÇϼ¼¿ä.
±â¿ø
Avian myeloblastosis virus (AMV)
Á¦Ç°¼³¸í
º» Á¦Ç°Àº AMV·ÎºÎÅÍ °íµµ·Î Á¤Á¦ÇÑ °ÍÀÌ´Ù. ¾à 10 kbÀÇ ±ä RNA (Poly(A)-tailed RNA Ladder (1.4~9.5 kb))¸¦ ÁÖÇüÀ¸·Î ÇÏ´Â cDNA ÇÕ¼º ¹ÝÀÀ°è¿¡¼­ 40~70% ÀÌ»óÀÌ ¿ªÀü»çµÇ¾î ±× Áß 55~80%°¡ ¿ÏÀüÇÑ ±æÀÌÀÇ cDNA ÇÕ¼ºÇÏ´Â °ÍÀ» marker¿¡¼­ È®ÀÎÇÏ¿´´Ù. º» Á¦Ç°Àº second strand ÇÕ¼º ÀúÇØÁ¦·Î½á actinomycin D À̿ܿ¡, 4 mMÀÇ sodium pyrophosphateµµ »ç¿ëÇÒ ¼ö ÀÖ´Â Á¡°ú ¹ÝÀÀ ÃÖÀû¿Âµµ°¡ ³ôÀº Á¡¿¡¼­ MMLV À¯·¡ÀÇ ¿ªÀü»çÈ¿¼Ò¿Í ´Ù¸£´Ù.
Ȱ¼ºÀÇ Á¤ÀÇ
Poly(rA)¡¤ oligo(dT)¸¦ °¢°¢ ÁÖÇü¡¤ ÇÁ¶óÀ̸ӷΠÇÏ¿© 37¡É¿¡¼­, 10ºÐ µ¿¾È 1 nmolÀÇ dTMP¸¦ ÇÕ¼º±âÁú·Î »ç¿ëÇÏ´Â °ÍÀ» È¿¼ÒȰ¼º 1 UÀ¸·Î ÇÑ´Ù.
Ȱ¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM Tris-HCl (pH8.3)
40 mM KCl
6 mM MgCl2
4 mM DTT
0.4 mM Poly(rA)¡¤ oligo(dT)12-18
0.5 mM [3H]dTTP
ǰÁú°ËÁ¤
1. cDNA ÇÕ¼º¹ÝÀÀ
poly(A)-tailed RNA Ladder(1.4~9.5 kb)¸¦ ÁÖÇüÀ¸·Î ÇÏ´Â cDNA ÇÕ¼º¹ÝÀÀ¿¡ ÀÖ¾î, ÁÖÇü DNAÀÇ 40%~70% ÀÌ»óÀÌ ¿ªÀü»çµÇ¾î ±× Áß 55%~80%°¡ ¿ÏÀüÇÑ ±æÀÌÀÌ´Ù.
2. RNase Assay
15 UÀÇ º» È¿¼Ò¿Í 1§¶ RNA Ladder (1.4~9.5 kb)¿Í 37¡É, 2½Ã°£ ¹ÝÀÀÇÏ¿©µµ RNA Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ÀϾÁö ¾Ê´Â´Ù.
3. DNase Assay
50 UÀÇ º» È¿¼Ò¿Í 0.5§¶¡¿174 RFI DNA Hae¥²´ÜÆíµéÀ» 37¡É, 1½Ã°£ ¹ÝÀÀÇÏ¿©µµ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ÀϾÁö ¾Ê´Â´Ù.
¿ëµµ
- 1st strand cDNAÀÇ ÇÕ¼º
- RNA-PCR
- cDNA probe Á¶Á¦

Keyword :

Reverse Transcription (Takara)
>
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
>
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
>
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
>
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
>
RT-PCR Çʼöǰ
Recombinant RNase Inhibitor
>
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
>
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer