200 mM
Potassium Phosphate, pH 7.2
2 mM
DTT
0.2 %
Triton X-100
50 %
Glycerol (v/v)
250 mM
Tris-HCl, pH 8.3
500 mM
KCl
20 mM
50 mM
MgCl2
40 mM
6 mM
4 mM
0.4 mM
poly (rA)¡¤(dT)12-18
05. mM
[3H]dTTP
2~4 U
RTase XL (AMV)