´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Premade cDNA > QUICK-Clone cDNA (Rat)

QUICK-Clone cDNA (Rat)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
637312
Rat Brain QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637314
Rat Heart QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637317
Rat Kidney QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637313
Rat Liver QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637318
Rat Pancreas QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637315
Rat Spleen QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982
Clontech
637316
Rat Testis QUICK-Clone cDNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ¡¿2
1,528,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32982

* º» Á¦Ç°Àº Àç°í ¼ÒÁø ÈÄ Á¾¸Å µÉ ¿¹Á¤ÀÌ¿À´Ï, ±¸¸Å Àü ¹Ýµå½Ã °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î Àç°í º¸À¯ À¯¹«¸¦ È®ÀΠ¹Ù¶ø´Ï´Ù.
QUICK-Clone cDNA
  • Library screening ¾øÀÌ PCR·Î Á÷Á¢ ¿øÇÏ´Â À¯ÀüÀÚ¸¦ cloning °¡´É
  • Human, mouse, ratÀÇ ´Ù¾çÇÑ Á¶Á÷°ú ¼¼Æ÷·ÎºÎÅÍ ÇÕ¼ºµÈ cDNA
  • ÀÌÀü¿¡ ºÐ¸®ÇÑ cDNA, ±¸Á¶ÀûÀ¸·Î °ü·ÃµÈ cDNA ¹× Á¾ °£ÀÇ cDNA¸¦ ÁõÆøÇϴµ¥ ÀûÇÕ
QUICK-Clone cDNA´Â À¯ÀüÀÚ Æ¯ÀÌÀûÀÎ primer·Î ¿øÇÏ´Â À¯ÀüÀÚ¸¦ ÁõÆøÇÒ ¼ö ÀÖµµ·Ï Á¦ÀÛµÈ double-strand cDNAÀÌ´Ù.
QUICK-Clone cDNA´Â ÀÌÀü¿¡ ºÐ¸®ÇÑ cDNA³ª ±¸Á¶ÀûÀ¸·Î °ü·ÃµÈ cDNA, cross-species cDNA¸¦ ÁõÆøÇϴµ¥ ÀûÇÕÇÏ´Ù. ¶ÇÇÑ, ¸ñÀû cDNAÀÇ ÁõÆøÀÇ À§ÇÏ¿© library¸¦ Á¦ÀÛÇÏ°í screeningÇÏ´Â ÀüÅëÀûÀÎ ¹æ¹ýÀ» »ç¿ëÇÏÁö ¾Ê¾Æµµ µÈ´Ù. ¶ÇÇÑ, QUICKClone cDNA´Â À¯ÀüÀÚ Æ¯ÀÌÀûÀÎ hybridization¿ë probe, degenerated primer¸¦ Á¦ÀÛÇÒ ¶§µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù (1~5).
°íÇ°ÁúÀÇ cDNA template´Â PCR ÁõÆø¿¡ ÁÁÀº °á°ú¸¦ °¡Á®¿Â´Ù. °íÇ°ÁúÀÇ ÇÁ¸®¹Ì¾ö poly A+ RNA·ÎºÎÅÍ oligo(dT) primer·Î ÇÕ¼ºµÈ QUICK-Clone cDNA´Â Á¤Á¦ °úÁ¤À» ÅëÇÏ¿© ÀÜ¿© RNA¿Í 400 bpÀÌÇÏÀÇ cDNA¸¦ ¸ðµÎ Á¦°ÅÇÏ¿© PCR¿¡ ÃÖÀûÀÎ °í¼øµµÀÇ double-strand cDNAÀÌ´Ù.
QUICK-Clone cDNA´Â °¢°¢ 10 ngÀÇ cDNA°¡ Æ÷ÇÔµÈ µÎ °³ÀÇ tube·Î Á¦°øµÇ¸ç, ÀÌ´Â 50 ¥ìl PCR ¹ÝÀÀ ½Ã 20ȸ¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Â ºÐ·®ÀÌ´Ù.
QUICK-Clone cDNA User Manual (PT1150-1)Àº www.clontech.com/manuals¿¡¼­ ´Ù¿î·Îµå ¹ÞÀ» ¼ö ÀÖ°í, PCRÀ» À§ÇÏ¿© TITANIUM Taq DNA Polymerase (Cat. Code 639208 & 639209) ¶Ç´Â ±ä ¼­¿­À» PCRÇÒ ¼ö ÀÖ´Â Advantage 2 Polymerase Mix (Cat. No. 639201 & 639202)¸¦ ÃßõÇÑ´Ù.
Full-length Human cDNAÀÇ È®º¸
QUICK-Clone II Human Universal cDNA (Cat. No. 639260)Àº ÀϹÝÀûÀÎ human Á¶Á÷¿¡¼­ ÇÕ¼ºµÈ 30Á¾ ÀÌ»óÀÇ QUICK-Clone cDNA°¡ ¼¯¿©ÀÖ´Â Á¦Ç°ÀÌ´Ù (Á¤È®ÇÑ ¼ýÀÚ´Â Á¶Á÷¿¡ µû¶ó ´Ù¸§). ƯÈ÷ ´ëºÎºÐÀÇ human À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏ´Â full-length cDNA ÁõÆø¿¡ ÀûÇÕÇÏ´Ù.

ÁÖ : »ç¿ëµÈ libraryÀÇ source´Â lotº°·Î ´Þ¶óÁú ¼ö ÀÖ´Ù. °¢°¢ÀÇ library source¿¡ ´ëÇÑ Á¤º¸´Â Certificate of Analysis¸¦ Âü°íÇϱ⠹ٶõ´Ù.

* QUICK-Clone XG Tumor cDNA´Â nude mouse¿¡¼­ Áõ½Ä½ÃŲ ÀÌÁ¾ÀÌ½Ä Àΰ£ Á¾¾ç (xenografted human tumors)¿¡¼­ ÇÕ¼ºµÇ¾ú´Ù. 6:149-158.

Figure 1. PCR amplification of the full coding region of the humanTFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. No.637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: ¥ë/Hind III DNA size marker. Lane M2: ¥ÕX174/Hae III DNA size marker.

7
Table I: Tissues Represented in QUICK-Clone II Human Universal cDNA
Adrenal Gland Fetal Lung Retina
Aorta Heart Salivary Gland
Bone MarrowKidney Skeletal Muscle
Brain Leukocyte Small Intestine
Brain, cerebellum Liver Spinal Cord
Brain, cerebral cortex Lung Spleen
Brain, hippocampus Lymph Node Stomach
Brain, thalamus Mammary Gland Testis
Fat Cell Ovary Thymus
Fetal Brain Pancreas Thyroid Gland
Fetal Heart Pituitary Gland Uterus
Fetal Kidney Placenta
Fetal Liver Prostate
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Parmentier, M. et al. (1989) Science 246:1620-1622.
2. Wilks, A. E. et al. (1989) Gene 85:67-74.
3. Vallins, W. J. et al. (1990) FEBS Letters 270:57-61.
4. Lee, C. C. et al. (1998) Science 239:1288-1291.
5. Schuchman, E. H. et al. (1990) Genomics
6:149-158.

Keyword :