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cDNA ÇÕ¼º
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SMART¢ç Full length cDNA ÇÕ¼º ¡¤ Library Á¦ÀÛ
> Marathon cDNA Amplification Kit
5¡¯-RACE¿Í 3¡¯-RACE·Î ºÎÅÍ cDNAÀ» È®º¸
Marathon cDNA Amplification Kit
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Clontech
634913
Marathon cDNA Amplification Kit
5 cDNA&100 PCRrxns
1,604,000¿ø
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Product index
Reverse Transcription (Tak..
High fidelity RTase, P..
High Fidelity, Full Le..
PrimeScript¢â IV 1st s..
PrimeScript¢â II 1st s..
PrimeScript¢â Reverse ..
PrimeScript¢â 1st stra..
Reverse Transcriptase ..
Recombinant RNase Inhi..
Reverse Transcriptase ..
Ribonuclease Inhibitor
cDNA Library Construct..
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (..
Double strand cDNA ÇÕ..
Oligo(dT) 15 primer
Recombinant RNase Inhi..
Reverse Transcription (Clo..
[°³¿ä] So Easy Product
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
MMLV Reverse Transcrip..
SMARTScribe Reverse Tr..
Advantage RT-for-PCR K..
SMART MMLV Reverse Tra..
TITANIUM One-Step RT-P..
in-vitro transcription
Takara IVTpro¢â mRNA S..
Vaccinia Capping Enzym..
mRNA Cap 2-O-Methyltra..
BspQ I
Poly(A) Polymerase
T7 RNA Polymerase ver...
Recombinant RNase Inhi..
Pyrophosphatase (inorg..
T7 RNA Polymerase
SP6 RNA Polymerase
Ribonucleoside 5£§-Tri..
SMART¢ç Full length cDNA ..
[°³¿ä] SMARTer¢ç cDNA ..
RACE 5/3 Kit (Unknown ..
Full length cDNA Synth..
Marathon cDNA Amplific..
cDNA Library Construct..
In-Fusion¢ç SMARTer cD..
cDNA Library (Human, Mouse..
Human Brain cDNA Libra..
Human cDNA Library
Mouse cDNA Library
Rat cDNA Library
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ ..
QUICK-Clone cDNA (Huma..
QUICK-Clone cDNA (Mous..
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
Total RNA£¦Poly A RNA
Human Tumor, Human Cel..
Premium Poly A+ RNA (H..
Premium Poly A+ RNA (H..
Premium Total RNA (Hum..
Premium Total RNA (Mou..
Premium Total RNA (Rat..
qPCR Human Reference c..
Total RNA Master Panel
Universal Reference To..
±× ¿Ü °ü·ÃÁ¦Ç° (RNA)
RNase-free Water
RNase-OFF¢ç
Library screening¾øÀÌ ½Å¼ÓÇÏ°Ô cDNA È®º¸ °¡´É
±ÍÂúÀº single-stranded ligation ¶Ç´Â tailing reactions ¾øÀ½
µ¿ÀÏÇÑ template¸¦ 5' RACE¿Í 3' RACE¿¡ µ¿½Ã¿¡ Àû¿ë °¡´É
ÀϹÝÀûÀÎ RACE Á¦Ç°µéÀº 5¡¯-RACE ¶Ç´Â 3¡¯-RACE, µÑ Áß ÇϳªÀÇ ½ÇÇè¿¡¸¸ Àû¿ëµÇµµ·Ï template°¡ Á¦Á¶µÈ´Ù. ±×·¯³ª
Marathon cDNA Amplification Kit
À» ÀÌ¿ëÇÏ¸é µ¿ÀÏÇÑ template¸¦ ÀÌ¿ëÇÏ¿© 5¡¯-RACE¿Í 3¡¯-RACE ¸ðµÎ¿¡ Àû¿ëÇÒ ¼ö ÀÖÀ¸¸ç Æ¯ÈµÈ adaptor¸¦ ä¿ëÇÏ¿© background¸¦ °¨¼Ò½ÃŲ´Ù. Marathon cDNA amplificationÀº expressed sequence tags (ESTs), differential display, RNA fingerprinting, ±×¸®°í cDNA subtraction°ú °°Àº ´Ù¾çÇÑ RNA Ư¼ºÀ» ½Å¼ÓÇÏ°Ô ÆÄ¾ÇÇϴµ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù. poly A+ RNA·ÎºÎÅÍ º¯ÇüµÈ lock-docking oligo(dT) primer¸¦ ÀÌ¿ëÇÏ¿© Marathon cDNA ÇÕ¼ºµÈ´Ù. º¯ÇüµÈ lock-docking oligo(dT) primer´Â 3¡¯-end¿¡ 2°³ÀÇ degenerate nucleotides¸¦ °®°í ÀÖ´Ù. cDNAÇÕ¼º ÈÄ blunt ends°¡ Çü¼ºµÇ°í Marathon adaptor°¡ double-stranded cDNA ¾ç¸»´Ü¿¡ ligationµÈ´Ù.
Clontech¿¡¼´Â °íǰÁúÀÇ ÇÁ¸®¹Ì¾ö poly A+ RNA¸¦ ÀÌ¿ëÇØ ÇÕ¼ºµÈ double-stranded cDNA¿¡ Marathon Adaptor°¡ ligationµÈ Marathon-Ready cDNAs¸¦ ÆÇ¸ÅÇϰí ÀÖ´Ù. ÀÌ Marathon-Ready cDNA¸¦ ÀÌ¿ëÇÏ¸é °£ÆíÇÏ°Ô RACE¸¦ ¼öÇàÇÒ ¼ö ÀÖ´Ù.
Applications
RACE (Rapid Amplification of cDNA Ends)
Identification of 3'/5' UTRs
Full-length gene cloning
Gene cloning and analysis
Additional Information
»ó´Ü ºñ°í¶õÀÇ more¸¦ Ŭ¸¯Çϼż clontech ȨÆäÀÌÁö Documents¿¡¼ Certificate of Analysis¿Í user manualÀ» È®ÀÎ ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Keyword :
SMART¢ç Full length cDNA ÇÕ¼º ¡¤ Library Á¦ÀÛ
[°³¿ä] SMARTer¢ç cDNA ÇÕ¼º±â¼ú (full length cDNA)
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
RACE 5/3 Kit (Unknown region analysis)
SMART¹ýÀ» ÀÌ¿ëÇÑ Full-length cDNA ÇÕ¼º
Full length cDNA Synthesis Kit
5¡¯-RACE¿Í 3¡¯-RACE·Î ºÎÅÍ cDNAÀ» È®º¸
Marathon cDNA Amplification Kit
cDNA Library Construction Kit
È®½ÇÇϰí È¿°úÀûÀÎ cDNA Library ±¸Ãà
In-Fusion¢ç SMARTer cDNA Library Construction Kit