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5¡¯-RACE¿Í 3¡¯-RACE·Î ºÎÅÍ cDNAÀ» È®º¸

Marathon cDNA Amplification Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634913
Marathon cDNA Amplification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 cDNA&100 PCRrxns
(Package)
1,334,400¿ø 
1,668,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32809, x32808

  • Library screening¾øÀÌ ½Å¼ÓÇÏ°Ô cDNA È®º¸ °¡´É
  • ±ÍÂúÀº single-stranded ligation ¶Ç´Â tailing reactions ¾øÀ½
  • µ¿ÀÏÇÑ template¸¦ 5' RACE¿Í 3' RACE¿¡ µ¿½Ã¿¡ Àû¿ë °¡´É

ÀϹÝÀûÀÎ RACE Á¦Ç°µéÀº 5¡¯-RACE ¶Ç´Â 3¡¯-RACE, µÑ Áß ÇϳªÀÇ ½ÇÇè¿¡¸¸ Àû¿ëµÇµµ·Ï template°¡ Á¦Á¶µÈ´Ù. ±×·¯³ª Marathon cDNA Amplification KitÀ» ÀÌ¿ëÇÏ¸é µ¿ÀÏÇÑ template¸¦ ÀÌ¿ëÇÏ¿© 5¡¯-RACE¿Í 3¡¯-RACE ¸ðµÎ¿¡ Àû¿ëÇÒ ¼ö ÀÖÀ¸¸ç ƯȭµÈ adaptor¸¦ ä¿ëÇÏ¿© background¸¦ °¨¼Ò½ÃŲ´Ù. Marathon cDNA amplificationÀº expressed sequence tags (ESTs), differential display, RNA fingerprinting, ±×¸®°í cDNA subtraction°ú °°Àº ´Ù¾çÇÑ RNA Ư¼ºÀ» ½Å¼ÓÇÏ°Ô ÆľÇÇϴµ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù. poly A+ RNA·ÎºÎÅÍ º¯ÇüµÈ lock-docking oligo(dT) primer¸¦ ÀÌ¿ëÇÏ¿© Marathon cDNA ÇÕ¼ºµÈ´Ù. º¯ÇüµÈ lock-docking oligo(dT) primer´Â 3¡¯-end¿¡ 2°³ÀÇ degenerate nucleotides¸¦ °®°í ÀÖ´Ù. cDNAÇÕ¼º ÈÄ blunt ends°¡ Çü¼ºµÇ°í Marathon adaptor°¡ double-stranded cDNA ¾ç¸»´Ü¿¡ ligationµÈ´Ù.

Clontech¿¡¼­´Â °íÇ°ÁúÀÇ ÇÁ¸®¹Ì¾ö poly A+ RNA¸¦ ÀÌ¿ëÇØ ÇÕ¼ºµÈ double-stranded cDNA¿¡ Marathon Adaptor°¡ ligationµÈ Marathon-Ready cDNAs¸¦ ÆǸÅÇÏ°í ÀÖ´Ù. ÀÌ Marathon-Ready cDNA¸¦ ÀÌ¿ëÇÏ¸é °£ÆíÇÏ°Ô RACE¸¦ ¼öÇàÇÒ ¼ö ÀÖ´Ù.
Applications
  • RACE (Rapid Amplification of cDNA Ends)
  • Identification of 3'/5' UTRs
  • Full-length gene cloning
  • Gene cloning and analysis
Additional Information
»ó´Ü ºñ°í¶õÀÇ more¸¦ Ŭ¸¯Çϼż­ clontech ȨÆäÀÌÁö Documents¿¡¼­ Certificate of Analysis¿Í user manualÀ» È®ÀÎ ÇÒ ¼ö ÀÖ½À´Ï´Ù.

Keyword :

SMART¢ç Full length cDNA ÇÕ¼º ¡¤ Library Á¦ÀÛ
>
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
RACE 5/3 Kit (Unknown region analysis)
>
SMART¹ýÀ» ÀÌ¿ëÇÑ Full-length cDNA ÇÕ¼º
Full length cDNA Synthesis Kit
>
5¡¯-RACE¿Í 3¡¯-RACE·Î ºÎÅÍ cDNAÀ» È®º¸
Marathon cDNA Amplification Kit