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cDNA ÇÕ¼º
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SMART¢ç Full length cDNA ÇÕ¼º ¡¤ Library Á¦ÀÛ
> RACE 5/3 Kit (Unknown region analysis)
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE Kit
RACE 5/3 Kit (Unknown region analysis)
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Clontech
634858
SMARTer¢ç RACE 5'/3' Kit
10 ȸ
(Package)
1,242,000¿ø
,
,
Clontech
634859
SMARTer¢ç RACE 5'/3' Kit
20 ȸ
(Package)
2,412,000¿ø
Clontech
634922
Universal Primer Mix
100 ȸ
290,000¿ø
Product index
Reverse Transcription (Tak..
High fidelity RTase, P..
High Fidelity, Full Le..
PrimeScript¢â IV 1st s..
PrimeScript¢â II 1st s..
PrimeScript¢â Reverse ..
PrimeScript¢â 1st stra..
Reverse Transcriptase ..
Recombinant RNase Inhi..
Reverse Transcriptase ..
Ribonuclease Inhibitor
cDNA Library Construct..
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (..
Double strand cDNA ÇÕ..
Oligo(dT) 15 primer
Reverse Transcription (Clo..
[°³¿ä] So Easy Product
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
MMLV Reverse Transcrip..
SMARTScribe Reverse Tr..
Advantage RT-for-PCR K..
SMART MMLV Reverse Tra..
TITANIUM One-Step RT-P..
SMART¢ç Full length cDNA ..
[°³¿ä] SMARTer¢ç cDNA ..
RACE 5/3 Kit (Unknown ..
Full length cDNA Synth..
Marathon cDNA Amplific..
cDNA Library (Human, Mouse..
Human cDNA Library
Rat cDNA Library
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ ..
QUICK-Clone cDNA (Huma..
QUICK-Clone cDNA (Mous..
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
Total RNA£¦Poly A RNA
Human Tumor, Human Cel..
Premium Poly A+ RNA (H..
Premium Poly A+ RNA (H..
Premium Total RNA (Hum..
Premium Total RNA (Mou..
Premium Total RNA (Rat..
qPCR Human Reference c..
Total RNA Master Panel
Universal Reference To..
±× ¿Ü °ü·ÃÁ¦Ç° (RNA)
RNase-free Water
RNase-OFF¢ç
Rapid Amplification of cDNA Ends (RACE)´Â RNAÀÇ full-length sequence¸¦ ¾ò±â À§ÇØ »ç¿ëµÇ´Â ±â¼úÀÌ´Ù. RACE´Â RNAÀÇ ÀϺΠ¾Ë°í ÀÖ´Â ºÎÀ§ (small known sequence)¸¦ ÀÌ¿ëÇÏ¿© ¹ÌÁöÀÇ 5¡¯ ¸»´Ü (5¡¯ RACE-PCR¹ý), 3¡¯ ¸»´Ü (3¡¯ RACE-PCR¹ý)À» ±Ô¸íÇϴµ¥ ÀûÇÕÇÑ ±â¼úÀÌ´Ù.
º» Á¦Ç° SMARTer RACE 5¡¯/3¡¯ KitÀº SMARTer ±â¼úÀ» ÀÌ¿ëÇÑ RACE cDNA ÇÕ¼º Å°Æ®·Î½á º°µµÀÇ adaptor ligation ¾øÀ̵µ ªÀº ½Ã°£ ³»¿¡ full-lengthÀÇ cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù. ÇÕ¼ºµÈ cDNA´Â 5¡¯ RACE-PCR°ú 3¡¯ RACE-PCR¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
SMARTer RACE 5¡¯/3¡¯ KitÀº ±âÁ¸ SMARTer RACE cDNA Amplification Kitº¸´Ù ´õ ³·Àº background¿Í ³ôÀº ƯÀ̼º(higher specificity)À» º¸ÀÌ´Â Á¦Ç°ÀÌ´Ù. ¶ÇÇÑ ±âÁ¸ Á¦Ç°¿¡ ºñÇÏ¿© ³óµµ°¡ ³·Àº RNA »ùÇà (Input volume up to 11ul)µµ »ç¿ë °¡´ÉÇϸç, Long fragmentÀ̰ųª GC-rich template¿Í °°Àº ÁõÆøÀÌ ¾î·Á¿î ¼¿µµ È¿À²ÀûÀ¸·Î ÁõÆø °¡´ÉÇÏ´Ù. º» Á¦Ç° ³»¿¡´Â SeqAmp DNA Polymerase, In-Fusion HD Cloning kit, linearized vector, competent cell±îÁö Æ÷ÇԵǾî ÀÖ´Ù.
Ư¡
Easy to Use -
performed in a single tube, and minimizes handling of both your RNA sample and your synthesized cDNA.
Complete Kit
- contains all the necessary components (save gene-specific primers) to streamline your process.
Requires Only 10 ng of Total RNA
- utilize small samples, including biopsies, tissue dissections, needle aspirations, and embryonic and rare disease tissues.
Specific Enrichment for 5' Ends
Our carefully designed, chemically-treated SMARTer Oligo preferentially hybridizes to the 5' ends of the cDNA being synthesized. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences.
No RNA Pretreatment Required
- requires no RNA pretreatment with DNase. This protocol works with total RNA, as well as with samples that may be contaminated with genomic DNA.
±×¸² 1. Overview of the SMARTer RACE 5'/3' Kit workflow
. Each kit is a complete system, containing the reagents required to recover cloned RACE fragments on the second day.
±×¸² 2. SMART(er) cDNA synthesis compared to conventional cDNA synthesis.
±×¸² 3. SMARTer 5'- and 3'-RACE results for different genes
. Lane 1: 1 kb DNA marker. Lane 2: 100 bp DNA marker. Lanes 3 & 4: tyrosyl-tRNA synthetase (YARS). Lanes 5 & 6: interferon gamma receptor 1 (IFNGR1). Lanes 7 & 8: adrenergic, beta, receptor kinase 1 (ADRBK1). Lanes 9 & 10: actin, beta (ACTB). Lanes 11 & 12: transferrin receptor (TFR) Lane 13: low density lipoprotein-related protein 2 (LRP2). Lane 14: low density lipoprotein-related protein 1 (LRP1).
Àû¿ë
RACE (
R
apid
A
mplification of
c
DNA
E
nds)
Identification of 3'/5' UTRs
Full-length gene cloning
Gene cloning and analysis
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
* SMARTer RACE 5¡¯/3¡¯ Kit Components (Cat. No. 634860) (Not sold separately)
* SeqAmp DNA Polymerase (Cat. No. 638504)
* In-Fusion HD Cloning Kit (Cat. No. 639648) (Not sold separately)
* NucleoSpin Gel and PCR Clean-Up Kit (Cat. No. 740609.10) (Not sold separately)
* Stellar Competent Cells (Cat. No. 636763)
Keyword :
SMART¢ç Full length cDNA ÇÕ¼º ¡¤ Library Á¦ÀÛ
[°³¿ä] SMARTer¢ç cDNA ÇÕ¼º±â¼ú (full length cDNA)
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
RACE 5/3 Kit (Unknown region analysis)
SMART¹ýÀ» ÀÌ¿ëÇÑ Full-length cDNA ÇÕ¼º
Full length cDNA Synthesis Kit
5¡¯-RACE¿Í 3¡¯-RACE·Î ºÎÅÍ cDNAÀ» È®º¸
Marathon cDNA Amplification Kit