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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Takara) > Reverse Transcriptase XL (AMV) for RT-PCR Kit
RNA PCR KitÀÇ Component

Reverse Transcriptase XL (AMV) for RT-PCR Kit

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*º» Á¦Ç°Àº ±¸¸Å Àü ¹Ýµå½Ã °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î ³³±â È®ÀÎ ¹Ù¶ø´Ï´Ù.
³óµµ 5 U/§¡
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10 mM Potassium Phosphate (pH7.2)
0.1 mM EDTA
2 mM DTT
0.2 % Triton X-100
50 % Glycerol
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-80¡É µ¿°áº¸Á¸ (µ¿°áÀ¶ÇØÀÇ ¹Ýº¹¿¡ µû¸¥ È°¼º ÀúÇÏ´Â ¾øÀ¸³ª °¡±ÞÀû ÇÇÇÒ °Í)
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º» Á¦Ç°Àº Avian Myeroblastosis Virus (AMV)·ÎºÎÅÍ °íµµ·Î Á¤Á¦ÇÑ °ÍÀÌ´Ù.
TaKaRa¿¡¼­ ÆǸÅÇÏ°í ÀÖ´Â RNA PCR Kit AMV RTase¿Í °°Àº ³óµµ (5 U/§¡)·Î Á¶Á¤ÇÑ °ÍÀÌ´Ù. RNA (PolyA-tailed RNA Ladder(1.4~9.5 kb)¸¦ ÁÖÇüÀ¸·Î ÇÏ´Â cDNAÇÕ¼º¹ÝÀÀ°è ÁÖÇüÀÇ 40~70%°¡ ¿ªÀü»çµÇ¾î ±× ÁßÀÇ 55~80%°¡ full length cDNA¸¦ ÇÕ¼ºÇÏ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.

¶Ç º» Á¦Ç°Àº ¾Æ·¡ Ç¥±âÇÑ Á¦Ç°¿¡ Æ÷ÇÔµÈ AMV Reverse Transcriptase XL°ú µ¿ÀÏÇÏ´Ù.
- TaKaRa RNA PCR Kit (AMV) Ver. 2.1(TaKaRa Code RR019A/B)
- TaKaRa RNA LA PCR Kit (AMV) Ver.1.1(TaKaRa Code RR012A)
- TaKaRa One Step RNA PCR Kit (AMV) (TaKaRa Code RR024A/B)
- mRNA Selective PCR Kit Ver.1.1(TaKaRa Code RR025A/B)
- 3¡¯- Full RACE Core Set (TaKaRa Code 6121)
- 5¡¯- Full RACE Core Set (TaKaRa Code 6122)
- Enzyme Set-FDD (TaKaRa Code R025A/B)
- Enzyme Set-DD (TaKaRa Code R024A/B)
È°¼ºÀÇ Á¤ÀÇ
Poly (rA)¡¤ oligo (dT)¸¦ °¢°¢ ÁÖÇü°ú ÇÁ¶óÀ̸ӷΠ37¡É¿¡¼­, 10ºÐ µ¿¾È 1 nmolÀÇ [3H]dTMP¸¦ »êºÒ¿ë¼º ħÀü¹°¿¡ ³Ö´Â È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM Tris-HCl (pH8.3)
40 mM KCl
6 mM MgCl2
4 mM DTT
0.4 mM Poly(rA)¡¤ oligo(dT)12-18
0.5 mM [3H]dTTP
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Reverse Transcriptase XL (AMV)(TaKaRa Code 2620A)°ú µ¿ÀÏÇÏ´Ù.

Keyword :

Reverse Transcription (Takara)
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